Repair of a segmental long bone defect in human by implantation of a novel multiple disc graft.
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Repair of a segmental long bone defect in human by implantation of a novel multiple disc graft. / Hesse, Eric; Kluge, Gerald; Atfi, Azeddine; Correa, Diego; Haasper, Carl; Berding, Georg; Shin, Hoen-oh; Viering, Jörg; Länger, Florian; Vogt, Peter M; Krettek, Christian; Jagodzinski, Michael.
in: BONE, Jahrgang 46, Nr. 5, 5, 2010, S. 1457-1463.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Repair of a segmental long bone defect in human by implantation of a novel multiple disc graft.
AU - Hesse, Eric
AU - Kluge, Gerald
AU - Atfi, Azeddine
AU - Correa, Diego
AU - Haasper, Carl
AU - Berding, Georg
AU - Shin, Hoen-oh
AU - Viering, Jörg
AU - Länger, Florian
AU - Vogt, Peter M
AU - Krettek, Christian
AU - Jagodzinski, Michael
PY - 2010
Y1 - 2010
N2 - Large segmental defects of the weight bearing long bones are very difficult to reconstruct. Current treatment options are afflicted with several limitations and disadvantages. We describe a novel approach to regenerate a segmental long bone defect in a patient using a multiple disc graft. Decellularized bovine trabecular bone discs were seeded with autologous bone marrow cells and cultured in a perfusion chamber for three weeks. Multiple cell-seeded discs were implanted to close a 72 mm defect of the distal tibia in a 58-year-old woman, and fixed by an intramedullary nail. Bone formation was assessed non-invasively by plain radiographs and 18F-labeled sodium fluoride-based co-registration of positron emission- and computed tomography (PET/CT). Bone was actively formed around the grafted defect as early as six weeks after surgery. Because the tibia was sufficiently stabilized, the patient was able to freely walk with full weight bearing 6 weeks after surgery. The uneventful two-year follow-up and the satisfaction of the patient demonstrated the success of the procedure. Therefore the use of multiple cell-seeded disc grafts can be considered as a treatment alternative for patients with segmental long bone defects.
AB - Large segmental defects of the weight bearing long bones are very difficult to reconstruct. Current treatment options are afflicted with several limitations and disadvantages. We describe a novel approach to regenerate a segmental long bone defect in a patient using a multiple disc graft. Decellularized bovine trabecular bone discs were seeded with autologous bone marrow cells and cultured in a perfusion chamber for three weeks. Multiple cell-seeded discs were implanted to close a 72 mm defect of the distal tibia in a 58-year-old woman, and fixed by an intramedullary nail. Bone formation was assessed non-invasively by plain radiographs and 18F-labeled sodium fluoride-based co-registration of positron emission- and computed tomography (PET/CT). Bone was actively formed around the grafted defect as early as six weeks after surgery. Because the tibia was sufficiently stabilized, the patient was able to freely walk with full weight bearing 6 weeks after surgery. The uneventful two-year follow-up and the satisfaction of the patient demonstrated the success of the procedure. Therefore the use of multiple cell-seeded disc grafts can be considered as a treatment alternative for patients with segmental long bone defects.
KW - Animals
KW - Humans
KW - Female
KW - Middle Aged
KW - Cattle
KW - Positron-Emission Tomography
KW - Bone Marrow Cells/cytology
KW - Regenerative Medicine
KW - Tibia/injuries/radiography/surgery
KW - Tissue Engineering/methods
KW - Transplantation, Autologous/methods
KW - Animals
KW - Humans
KW - Female
KW - Middle Aged
KW - Cattle
KW - Positron-Emission Tomography
KW - Bone Marrow Cells/cytology
KW - Regenerative Medicine
KW - Tibia/injuries/radiography/surgery
KW - Tissue Engineering/methods
KW - Transplantation, Autologous/methods
M3 - SCORING: Journal article
VL - 46
SP - 1457
EP - 1463
JO - BONE
JF - BONE
SN - 8756-3282
IS - 5
M1 - 5
ER -
