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Renal intercalated cells are rather energized by a proton than a sodium pump

Standard

Renal intercalated cells are rather energized by a proton than a sodium pump. / Chambrey, Régine; Kurth, Ingo; Peti-Peterdi, Janos; Houillier, Pascal; Purkerson, Jeffrey M; Leviel, Françoise; Hentschke, Moritz; Zdebik, Anselm A; Schwartz, George J; Hübner, Christian A; Eladari, Dominique.

in: P NATL ACAD SCI USA, Jahrgang 110, Nr. 19, 07.05.2013, S. 7928-33.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Chambrey, R, Kurth, I, Peti-Peterdi, J, Houillier, P, Purkerson, JM, Leviel, F, Hentschke, M, Zdebik, AA, Schwartz, GJ, Hübner, CA & Eladari, D 2013, 'Renal intercalated cells are rather energized by a proton than a sodium pump', P NATL ACAD SCI USA, Jg. 110, Nr. 19, S. 7928-33. https://doi.org/10.1073/pnas.1221496110

APA

Chambrey, R., Kurth, I., Peti-Peterdi, J., Houillier, P., Purkerson, J. M., Leviel, F., Hentschke, M., Zdebik, A. A., Schwartz, G. J., Hübner, C. A., & Eladari, D. (2013). Renal intercalated cells are rather energized by a proton than a sodium pump. P NATL ACAD SCI USA, 110(19), 7928-33. https://doi.org/10.1073/pnas.1221496110

Vancouver

Chambrey R, Kurth I, Peti-Peterdi J, Houillier P, Purkerson JM, Leviel F et al. Renal intercalated cells are rather energized by a proton than a sodium pump. P NATL ACAD SCI USA. 2013 Mai 7;110(19):7928-33. https://doi.org/10.1073/pnas.1221496110

Bibtex

@article{4d256e99ab2c4b7785ad455c15292548,
title = "Renal intercalated cells are rather energized by a proton than a sodium pump",
abstract = "The Na(+) concentration of the intracellular milieu is very low compared with the extracellular medium. Transport of Na(+) along this gradient is used to fuel secondary transport of many solutes, and thus plays a major role for most cell functions including the control of cell volume and resting membrane potential. Because of a continuous leak, Na(+) has to be permanently removed from the intracellular milieu, a process that is thought to be exclusively mediated by the Na(+)/K(+)-ATPase in animal cells. Here, we show that intercalated cells of the mouse kidney are an exception to this general rule. By an approach combining two-photon imaging of isolated renal tubules, physiological studies, and genetically engineered animals, we demonstrate that inhibition of the H(+) vacuolar-type ATPase (V-ATPase) caused drastic cell swelling and depolarization, and also inhibited the NaCl absorption pathway that we recently discovered in intercalated cells. In contrast, pharmacological blockade of the Na(+)/K(+)-ATPase had no effects. Basolateral NaCl exit from β-intercalated cells was independent of the Na(+)/K(+)-ATPase but critically relied on the presence of the basolateral ion transporter anion exchanger 4. We conclude that not all animal cells critically rely on the sodium pump as the unique bioenergizer, but can be replaced by the H(+) V-ATPase in renal intercalated cells. This concept is likely to apply to other animal cell types characterized by plasma membrane expression of the H(+) V-ATPase.",
keywords = "Absorption, Animals, Cell Membrane, Cells, Cultured, Chloride-Bicarbonate Antiporters, Immunohistochemistry, Ions, Kidney, Membrane Potentials, Mice, Mice, Knockout, Perfusion, Proton Pumps, Sodium, Sodium Chloride, Sodium-Potassium-Exchanging ATPase, Vacuolar Proton-Translocating ATPases",
author = "R{\'e}gine Chambrey and Ingo Kurth and Janos Peti-Peterdi and Pascal Houillier and Purkerson, {Jeffrey M} and Fran{\c c}oise Leviel and Moritz Hentschke and Zdebik, {Anselm A} and Schwartz, {George J} and H{\"u}bner, {Christian A} and Dominique Eladari",
year = "2013",
month = may,
day = "7",
doi = "10.1073/pnas.1221496110",
language = "English",
volume = "110",
pages = "7928--33",
journal = "P NATL ACAD SCI USA",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "19",

}

RIS

TY - JOUR

T1 - Renal intercalated cells are rather energized by a proton than a sodium pump

AU - Chambrey, Régine

AU - Kurth, Ingo

AU - Peti-Peterdi, Janos

AU - Houillier, Pascal

AU - Purkerson, Jeffrey M

AU - Leviel, Françoise

AU - Hentschke, Moritz

AU - Zdebik, Anselm A

AU - Schwartz, George J

AU - Hübner, Christian A

AU - Eladari, Dominique

PY - 2013/5/7

Y1 - 2013/5/7

N2 - The Na(+) concentration of the intracellular milieu is very low compared with the extracellular medium. Transport of Na(+) along this gradient is used to fuel secondary transport of many solutes, and thus plays a major role for most cell functions including the control of cell volume and resting membrane potential. Because of a continuous leak, Na(+) has to be permanently removed from the intracellular milieu, a process that is thought to be exclusively mediated by the Na(+)/K(+)-ATPase in animal cells. Here, we show that intercalated cells of the mouse kidney are an exception to this general rule. By an approach combining two-photon imaging of isolated renal tubules, physiological studies, and genetically engineered animals, we demonstrate that inhibition of the H(+) vacuolar-type ATPase (V-ATPase) caused drastic cell swelling and depolarization, and also inhibited the NaCl absorption pathway that we recently discovered in intercalated cells. In contrast, pharmacological blockade of the Na(+)/K(+)-ATPase had no effects. Basolateral NaCl exit from β-intercalated cells was independent of the Na(+)/K(+)-ATPase but critically relied on the presence of the basolateral ion transporter anion exchanger 4. We conclude that not all animal cells critically rely on the sodium pump as the unique bioenergizer, but can be replaced by the H(+) V-ATPase in renal intercalated cells. This concept is likely to apply to other animal cell types characterized by plasma membrane expression of the H(+) V-ATPase.

AB - The Na(+) concentration of the intracellular milieu is very low compared with the extracellular medium. Transport of Na(+) along this gradient is used to fuel secondary transport of many solutes, and thus plays a major role for most cell functions including the control of cell volume and resting membrane potential. Because of a continuous leak, Na(+) has to be permanently removed from the intracellular milieu, a process that is thought to be exclusively mediated by the Na(+)/K(+)-ATPase in animal cells. Here, we show that intercalated cells of the mouse kidney are an exception to this general rule. By an approach combining two-photon imaging of isolated renal tubules, physiological studies, and genetically engineered animals, we demonstrate that inhibition of the H(+) vacuolar-type ATPase (V-ATPase) caused drastic cell swelling and depolarization, and also inhibited the NaCl absorption pathway that we recently discovered in intercalated cells. In contrast, pharmacological blockade of the Na(+)/K(+)-ATPase had no effects. Basolateral NaCl exit from β-intercalated cells was independent of the Na(+)/K(+)-ATPase but critically relied on the presence of the basolateral ion transporter anion exchanger 4. We conclude that not all animal cells critically rely on the sodium pump as the unique bioenergizer, but can be replaced by the H(+) V-ATPase in renal intercalated cells. This concept is likely to apply to other animal cell types characterized by plasma membrane expression of the H(+) V-ATPase.

KW - Absorption

KW - Animals

KW - Cell Membrane

KW - Cells, Cultured

KW - Chloride-Bicarbonate Antiporters

KW - Immunohistochemistry

KW - Ions

KW - Kidney

KW - Membrane Potentials

KW - Mice

KW - Mice, Knockout

KW - Perfusion

KW - Proton Pumps

KW - Sodium

KW - Sodium Chloride

KW - Sodium-Potassium-Exchanging ATPase

KW - Vacuolar Proton-Translocating ATPases

U2 - 10.1073/pnas.1221496110

DO - 10.1073/pnas.1221496110

M3 - SCORING: Journal article

C2 - 23610411

VL - 110

SP - 7928

EP - 7933

JO - P NATL ACAD SCI USA

JF - P NATL ACAD SCI USA

SN - 0027-8424

IS - 19

ER -