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RelA mutation and pBR322 plasmid amplification in amino acid-starved cells of Escherichia coli.

Standard

RelA mutation and pBR322 plasmid amplification in amino acid-starved cells of Escherichia coli. / Riethdorf, Sabine; Schroeter, A; Hecker, M.

in: GENET RES, Jahrgang 54, Nr. 3, 3, 1989, S. 167-171.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Riethdorf, S, Schroeter, A & Hecker, M 1989, 'RelA mutation and pBR322 plasmid amplification in amino acid-starved cells of Escherichia coli.', GENET RES, Jg. 54, Nr. 3, 3, S. 167-171. <http://www.ncbi.nlm.nih.gov/pubmed/2695389?dopt=Citation>

APA

Riethdorf, S., Schroeter, A., & Hecker, M. (1989). RelA mutation and pBR322 plasmid amplification in amino acid-starved cells of Escherichia coli. GENET RES, 54(3), 167-171. [3]. http://www.ncbi.nlm.nih.gov/pubmed/2695389?dopt=Citation

Vancouver

Riethdorf S, Schroeter A, Hecker M. RelA mutation and pBR322 plasmid amplification in amino acid-starved cells of Escherichia coli. GENET RES. 1989;54(3):167-171. 3.

Bibtex

@article{1292324ed5cf408da9a2f616bd4cc4c6,
title = "RelA mutation and pBR322 plasmid amplification in amino acid-starved cells of Escherichia coli.",
abstract = "Plasmid pBR322 is amplified following amino-acid limitation in Escherichia coli relA hosts. In relA+ hosts there was no significant amplification or a much smaller one. Plasmid amplification is due to the relA mutation; when the relA+ allele is transferred into the relA mutant CP79 this strain no longer amplifies plasmid DNA during amino acid starvation. It is concluded that ppGpp is a negative effector of plasmid replication. Amplification is temperature dependent, being maximal at 32 degrees C and negligible at 37 degrees C.",
keywords = "Mutation, Temperature, *Gene Amplification, Escherichia coli/*genetics, *Plasmids, Amino Acids/pharmacology, Culture Media, *Genes, Bacterial, Mutation, Temperature, *Gene Amplification, Escherichia coli/*genetics, *Plasmids, Amino Acids/pharmacology, Culture Media, *Genes, Bacterial",
author = "Sabine Riethdorf and A Schroeter and M Hecker",
year = "1989",
language = "English",
volume = "54",
pages = "167--171",
number = "3",

}

RIS

TY - JOUR

T1 - RelA mutation and pBR322 plasmid amplification in amino acid-starved cells of Escherichia coli.

AU - Riethdorf, Sabine

AU - Schroeter, A

AU - Hecker, M

PY - 1989

Y1 - 1989

N2 - Plasmid pBR322 is amplified following amino-acid limitation in Escherichia coli relA hosts. In relA+ hosts there was no significant amplification or a much smaller one. Plasmid amplification is due to the relA mutation; when the relA+ allele is transferred into the relA mutant CP79 this strain no longer amplifies plasmid DNA during amino acid starvation. It is concluded that ppGpp is a negative effector of plasmid replication. Amplification is temperature dependent, being maximal at 32 degrees C and negligible at 37 degrees C.

AB - Plasmid pBR322 is amplified following amino-acid limitation in Escherichia coli relA hosts. In relA+ hosts there was no significant amplification or a much smaller one. Plasmid amplification is due to the relA mutation; when the relA+ allele is transferred into the relA mutant CP79 this strain no longer amplifies plasmid DNA during amino acid starvation. It is concluded that ppGpp is a negative effector of plasmid replication. Amplification is temperature dependent, being maximal at 32 degrees C and negligible at 37 degrees C.

KW - Mutation

KW - Temperature

KW - Gene Amplification

KW - Escherichia coli/genetics

KW - Plasmids

KW - Amino Acids/pharmacology

KW - Culture Media

KW - Genes, Bacterial

KW - Mutation

KW - Temperature

KW - Gene Amplification

KW - Escherichia coli/genetics

KW - Plasmids

KW - Amino Acids/pharmacology

KW - Culture Media

KW - Genes, Bacterial

M3 - SCORING: Journal article

VL - 54

SP - 167

EP - 171

IS - 3

M1 - 3

ER -