Regulation of the renin expression in the retinal pigment epithelium by systemic stimuli

Vladimir M Milenkovic; Marisa Brockmann; Christian Meyer; Michael Desch; Frank Schweda; Armin Kurtz; Vladimir Todorov; Olaf Strauss

doi:10.1152/ajprenal.00576.2009

Regulation of the renin expression in the retinal pigment epithelium by systemic stimuli

Standard

Regulation of the renin expression in the retinal pigment epithelium by systemic stimuli. / Milenkovic, Vladimir M; Brockmann, Marisa; Meyer, Christian; Desch, Michael; Schweda, Frank; Kurtz, Armin; Todorov, Vladimir; Strauss, Olaf.

in: AM J PHYSIOL-RENAL, Jahrgang 299, Nr. 2, 2, 08.2010, S. 396-403.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Milenkovic, VM, Brockmann, M, Meyer, C, Desch, M, Schweda, F, Kurtz, A, Todorov, V & Strauss, O 2010, 'Regulation of the renin expression in the retinal pigment epithelium by systemic stimuli', AM J PHYSIOL-RENAL, Jg. 299, Nr. 2, 2, S. 396-403. https://doi.org/10.1152/ajprenal.00576.2009

APA

Milenkovic, V. M., Brockmann, M., Meyer, C., Desch, M., Schweda, F., Kurtz, A., Todorov, V., & Strauss, O. (2010). Regulation of the renin expression in the retinal pigment epithelium by systemic stimuli. AM J PHYSIOL-RENAL, 299(2), 396-403. [2]. https://doi.org/10.1152/ajprenal.00576.2009

Vancouver

Milenkovic VM, Brockmann M, Meyer C, Desch M, Schweda F, Kurtz A et al. Regulation of the renin expression in the retinal pigment epithelium by systemic stimuli. AM J PHYSIOL-RENAL. 2010 Aug;299(2):396-403. 2. https://doi.org/10.1152/ajprenal.00576.2009

Bibtex

@article{1494a19b95924c648a0f80f087d2d9a4,

title = "Regulation of the renin expression in the retinal pigment epithelium by systemic stimuli",

abstract = "The retina expresses a local renin-angiotensin system (RAS). This study aimed to investigate the influence of systemic modulation of renin synthesis on the expression of renin in the retinal pigment epithelium (RPE), which forms part of the blood/retina barrier. Freshly isolated RPE cells showed expression of renin 1A, which is the secreted isoform of renin. Systemic administration of the angiotensin-converting enzyme inhibitor enalapril in mice increased the renin expression in both the kidney and the retina. Systemic infusion of ANG II led to a decrease in the renin expression in the kidney and in the retina and RPE. The ANG II-dependent down-regulation of renin expression in the RPE was prevented by systemic application of the AT(1) receptor blocker losartan. However, water deprivation lead to an increase of the renin expression in the kidney but unexpectedly to a decrease of the renin expression in the retina. In sections of the mouse retina, the ANG II receptor AT(1) was found in the RPE and localized at the blood side of the epithelium. Short-time cultured RPE cells showed increases in intracellular free Ca(2+) in response to stimulation by ANG II that were sensitive to losartan. In summary, we conclude that the renin expression in cells of the blood/retina barrier is influenced by the systemic RAS. ANG II circulating in the plasma is likely a mediator of this influence.",

keywords = "Animals, dosage, Time Factors, Cells, Cultured, Mice, Kidney drug effects, Calcium metabolism, Retinal Pigment Epithelium drug effects, Administration, Oral, Angiotensin II administration, Angiotensin II Type 1 Receptor Blockers administration, Angiotensin-Converting Enzyme Inhibitors administration, Blood-Retinal Barrier drug effects, Enalapril administration, Epithelial Cells drug effects, Infusion Pumps, Implantable, Infusions, Subcutaneous, Losartan administration, Renin metabolism, Renin-Angiotensin System drug effects, Water Deprivation, Animals, dosage, Time Factors, Cells, Cultured, Mice, Kidney drug effects, Calcium metabolism, Retinal Pigment Epithelium drug effects, Administration, Oral, Angiotensin II administration, Angiotensin II Type 1 Receptor Blockers administration, Angiotensin-Converting Enzyme Inhibitors administration, Blood-Retinal Barrier drug effects, Enalapril administration, Epithelial Cells drug effects, Infusion Pumps, Implantable, Infusions, Subcutaneous, Losartan administration, Renin metabolism, Renin-Angiotensin System drug effects, Water Deprivation",

author = "Milenkovic, {Vladimir M} and Marisa Brockmann and Christian Meyer and Michael Desch and Frank Schweda and Armin Kurtz and Vladimir Todorov and Olaf Strauss",

year = "2010",

month = aug,

doi = "10.1152/ajprenal.00576.2009",

language = "English",

volume = "299",

pages = "396--403",

journal = "AM J PHYSIOL-RENAL",

issn = "1931-857X",

publisher = "AMER PHYSIOLOGICAL SOC",

number = "2",

}

RIS

TY - JOUR

T1 - Regulation of the renin expression in the retinal pigment epithelium by systemic stimuli

AU - Milenkovic, Vladimir M

AU - Brockmann, Marisa

AU - Meyer, Christian

AU - Desch, Michael

AU - Schweda, Frank

AU - Kurtz, Armin

AU - Todorov, Vladimir

AU - Strauss, Olaf

PY - 2010/8

Y1 - 2010/8

N2 - The retina expresses a local renin-angiotensin system (RAS). This study aimed to investigate the influence of systemic modulation of renin synthesis on the expression of renin in the retinal pigment epithelium (RPE), which forms part of the blood/retina barrier. Freshly isolated RPE cells showed expression of renin 1A, which is the secreted isoform of renin. Systemic administration of the angiotensin-converting enzyme inhibitor enalapril in mice increased the renin expression in both the kidney and the retina. Systemic infusion of ANG II led to a decrease in the renin expression in the kidney and in the retina and RPE. The ANG II-dependent down-regulation of renin expression in the RPE was prevented by systemic application of the AT(1) receptor blocker losartan. However, water deprivation lead to an increase of the renin expression in the kidney but unexpectedly to a decrease of the renin expression in the retina. In sections of the mouse retina, the ANG II receptor AT(1) was found in the RPE and localized at the blood side of the epithelium. Short-time cultured RPE cells showed increases in intracellular free Ca(2+) in response to stimulation by ANG II that were sensitive to losartan. In summary, we conclude that the renin expression in cells of the blood/retina barrier is influenced by the systemic RAS. ANG II circulating in the plasma is likely a mediator of this influence.

AB - The retina expresses a local renin-angiotensin system (RAS). This study aimed to investigate the influence of systemic modulation of renin synthesis on the expression of renin in the retinal pigment epithelium (RPE), which forms part of the blood/retina barrier. Freshly isolated RPE cells showed expression of renin 1A, which is the secreted isoform of renin. Systemic administration of the angiotensin-converting enzyme inhibitor enalapril in mice increased the renin expression in both the kidney and the retina. Systemic infusion of ANG II led to a decrease in the renin expression in the kidney and in the retina and RPE. The ANG II-dependent down-regulation of renin expression in the RPE was prevented by systemic application of the AT(1) receptor blocker losartan. However, water deprivation lead to an increase of the renin expression in the kidney but unexpectedly to a decrease of the renin expression in the retina. In sections of the mouse retina, the ANG II receptor AT(1) was found in the RPE and localized at the blood side of the epithelium. Short-time cultured RPE cells showed increases in intracellular free Ca(2+) in response to stimulation by ANG II that were sensitive to losartan. In summary, we conclude that the renin expression in cells of the blood/retina barrier is influenced by the systemic RAS. ANG II circulating in the plasma is likely a mediator of this influence.

KW - Animals

KW - dosage

KW - Time Factors

KW - Cells, Cultured

KW - Mice

KW - Kidney drug effects

KW - Calcium metabolism

KW - Retinal Pigment Epithelium drug effects

KW - Administration, Oral

KW - Angiotensin II administration

KW - Angiotensin II Type 1 Receptor Blockers administration

KW - Angiotensin-Converting Enzyme Inhibitors administration

KW - Blood-Retinal Barrier drug effects

KW - Enalapril administration

KW - Epithelial Cells drug effects

KW - Infusion Pumps, Implantable

KW - Infusions, Subcutaneous

KW - Losartan administration

KW - Renin metabolism

KW - Renin-Angiotensin System drug effects

KW - Water Deprivation

KW - Animals

KW - dosage

KW - Time Factors

KW - Cells, Cultured

KW - Mice

KW - Kidney drug effects

KW - Calcium metabolism

KW - Retinal Pigment Epithelium drug effects

KW - Administration, Oral

KW - Angiotensin II administration

KW - Angiotensin II Type 1 Receptor Blockers administration

KW - Angiotensin-Converting Enzyme Inhibitors administration

KW - Blood-Retinal Barrier drug effects

KW - Enalapril administration

KW - Epithelial Cells drug effects

KW - Infusion Pumps, Implantable

KW - Infusions, Subcutaneous

KW - Losartan administration

KW - Renin metabolism

KW - Renin-Angiotensin System drug effects

KW - Water Deprivation

U2 - 10.1152/ajprenal.00576.2009

DO - 10.1152/ajprenal.00576.2009

M3 - SCORING: Journal article

C2 - 20519377

VL - 299

SP - 396

EP - 403

JO - AM J PHYSIOL-RENAL

JF - AM J PHYSIOL-RENAL

SN - 1931-857X

IS - 2

M1 - 2

ER -