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Regulation of expression of the retinoic acid-synthesising enzymes retinaldehyde dehydrogenases in the uteri of ovariectomised mice after treatment with oestrogen, gestagen and their combination

Standard

Regulation of expression of the retinoic acid-synthesising enzymes retinaldehyde dehydrogenases in the uteri of ovariectomised mice after treatment with oestrogen, gestagen and their combination. / Rühl, Ralph; Fritzsche, Britta; Vermot, Julien; Niederreither, Karen; Neumann, Ulrike; Schmidt, Anja; Schweigert, Florian J; Dollé, Pascal.

in: REPROD FERT DEVELOP, Jahrgang 18, Nr. 3, 2006, S. 339-45.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung

Harvard

Rühl, R, Fritzsche, B, Vermot, J, Niederreither, K, Neumann, U, Schmidt, A, Schweigert, FJ & Dollé, P 2006, 'Regulation of expression of the retinoic acid-synthesising enzymes retinaldehyde dehydrogenases in the uteri of ovariectomised mice after treatment with oestrogen, gestagen and their combination', REPROD FERT DEVELOP, Jg. 18, Nr. 3, S. 339-45.

APA

Rühl, R., Fritzsche, B., Vermot, J., Niederreither, K., Neumann, U., Schmidt, A., Schweigert, F. J., & Dollé, P. (2006). Regulation of expression of the retinoic acid-synthesising enzymes retinaldehyde dehydrogenases in the uteri of ovariectomised mice after treatment with oestrogen, gestagen and their combination. REPROD FERT DEVELOP, 18(3), 339-45.

Vancouver

Rühl R, Fritzsche B, Vermot J, Niederreither K, Neumann U, Schmidt A et al. Regulation of expression of the retinoic acid-synthesising enzymes retinaldehyde dehydrogenases in the uteri of ovariectomised mice after treatment with oestrogen, gestagen and their combination. REPROD FERT DEVELOP. 2006;18(3):339-45.

Bibtex

@article{0d2ef232a5e04c84914ee078dd8ee788,
title = "Regulation of expression of the retinoic acid-synthesising enzymes retinaldehyde dehydrogenases in the uteri of ovariectomised mice after treatment with oestrogen, gestagen and their combination",
abstract = "The active metabolite of vitamin A, retinoic acid (RA), plays an important role in the female reproductive system. The synthesis of RA is tightly regulated by the activity of retinaldehyde dehydrogenases (Raldh). Among these, Raldh1 and Raldh2 exhibit specific temporal and spatial expression patterns in the mouse uterus, both during the oestrous cycle and early pregnancy. In the present study, we have assessed whether oestradiol and progesterone directly influence the uterine expression of Raldh1 and Raldh2 in ovariectomised mice. We investigated the effect of gestagen (promegestone 0.3 mg kg(-1) bodyweight), oestrogen (oestradiol 3 microg kg(-1) bodyweight) and their combination on the uterine expression of Raldh2. Expression was analysed using in situ hybridisation and quantified using real-time detection reverse transcription-polymerase chain reaction. The results show that the expression of Raldh2 is rapidly (within 1-4 h) induced in stromal cells by oestrogen, but not by gestagen, treatment, whereas combined oestrogen + gestagen treatment leads to a more prolonged (48 h) response. In contrast, oestrogen, but not progesterone, treatment downregulates (within 4-24 h) Raldh1 expression in the uterine glandular epithelium. We conclude that the uterine RA concentrations are regulated by oestrogens via an effect on the expression of the Raldh synthesising enzymes. Such a regulation is consistent with the natural fluctuations of Raldh expression during the oestrous cycle, early pregnancy and blastocyst implantation.",
keywords = "Aldehyde Oxidoreductases, Animals, Drug Interactions, Estradiol, Female, Gene Expression, In Situ Hybridization, Isoenzymes, Mice, Ovariectomy, Progesterone, RNA, Messenger, Retinal Dehydrogenase, Reverse Transcriptase Polymerase Chain Reaction, Uterus, Journal Article, Research Support, Non-U.S. Gov't",
author = "Ralph R{\"u}hl and Britta Fritzsche and Julien Vermot and Karen Niederreither and Ulrike Neumann and Anja Schmidt and Schweigert, {Florian J} and Pascal Doll{\'e}",
year = "2006",
language = "English",
volume = "18",
pages = "339--45",
journal = "REPROD FERT DEVELOP",
issn = "1031-3613",
publisher = "CSIRO",
number = "3",

}

RIS

TY - JOUR

T1 - Regulation of expression of the retinoic acid-synthesising enzymes retinaldehyde dehydrogenases in the uteri of ovariectomised mice after treatment with oestrogen, gestagen and their combination

AU - Rühl, Ralph

AU - Fritzsche, Britta

AU - Vermot, Julien

AU - Niederreither, Karen

AU - Neumann, Ulrike

AU - Schmidt, Anja

AU - Schweigert, Florian J

AU - Dollé, Pascal

PY - 2006

Y1 - 2006

N2 - The active metabolite of vitamin A, retinoic acid (RA), plays an important role in the female reproductive system. The synthesis of RA is tightly regulated by the activity of retinaldehyde dehydrogenases (Raldh). Among these, Raldh1 and Raldh2 exhibit specific temporal and spatial expression patterns in the mouse uterus, both during the oestrous cycle and early pregnancy. In the present study, we have assessed whether oestradiol and progesterone directly influence the uterine expression of Raldh1 and Raldh2 in ovariectomised mice. We investigated the effect of gestagen (promegestone 0.3 mg kg(-1) bodyweight), oestrogen (oestradiol 3 microg kg(-1) bodyweight) and their combination on the uterine expression of Raldh2. Expression was analysed using in situ hybridisation and quantified using real-time detection reverse transcription-polymerase chain reaction. The results show that the expression of Raldh2 is rapidly (within 1-4 h) induced in stromal cells by oestrogen, but not by gestagen, treatment, whereas combined oestrogen + gestagen treatment leads to a more prolonged (48 h) response. In contrast, oestrogen, but not progesterone, treatment downregulates (within 4-24 h) Raldh1 expression in the uterine glandular epithelium. We conclude that the uterine RA concentrations are regulated by oestrogens via an effect on the expression of the Raldh synthesising enzymes. Such a regulation is consistent with the natural fluctuations of Raldh expression during the oestrous cycle, early pregnancy and blastocyst implantation.

AB - The active metabolite of vitamin A, retinoic acid (RA), plays an important role in the female reproductive system. The synthesis of RA is tightly regulated by the activity of retinaldehyde dehydrogenases (Raldh). Among these, Raldh1 and Raldh2 exhibit specific temporal and spatial expression patterns in the mouse uterus, both during the oestrous cycle and early pregnancy. In the present study, we have assessed whether oestradiol and progesterone directly influence the uterine expression of Raldh1 and Raldh2 in ovariectomised mice. We investigated the effect of gestagen (promegestone 0.3 mg kg(-1) bodyweight), oestrogen (oestradiol 3 microg kg(-1) bodyweight) and their combination on the uterine expression of Raldh2. Expression was analysed using in situ hybridisation and quantified using real-time detection reverse transcription-polymerase chain reaction. The results show that the expression of Raldh2 is rapidly (within 1-4 h) induced in stromal cells by oestrogen, but not by gestagen, treatment, whereas combined oestrogen + gestagen treatment leads to a more prolonged (48 h) response. In contrast, oestrogen, but not progesterone, treatment downregulates (within 4-24 h) Raldh1 expression in the uterine glandular epithelium. We conclude that the uterine RA concentrations are regulated by oestrogens via an effect on the expression of the Raldh synthesising enzymes. Such a regulation is consistent with the natural fluctuations of Raldh expression during the oestrous cycle, early pregnancy and blastocyst implantation.

KW - Aldehyde Oxidoreductases

KW - Animals

KW - Drug Interactions

KW - Estradiol

KW - Female

KW - Gene Expression

KW - In Situ Hybridization

KW - Isoenzymes

KW - Mice

KW - Ovariectomy

KW - Progesterone

KW - RNA, Messenger

KW - Retinal Dehydrogenase

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Uterus

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - SCORING: Journal article

C2 - 16554009

VL - 18

SP - 339

EP - 345

JO - REPROD FERT DEVELOP

JF - REPROD FERT DEVELOP

SN - 1031-3613

IS - 3

ER -