Regulation of cell volume by glycosaminoglycans.
Standard
Regulation of cell volume by glycosaminoglycans. / Joerges, Jelena; Schulz, Tobias; Wegner, Jeannine; Schumacher, Udo; Prehm, Peter.
in: J CELL BIOCHEM, Jahrgang 113, Nr. 1, 1, 2012, S. 340-348.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Regulation of cell volume by glycosaminoglycans.
AU - Joerges, Jelena
AU - Schulz, Tobias
AU - Wegner, Jeannine
AU - Schumacher, Udo
AU - Prehm, Peter
PY - 2012
Y1 - 2012
N2 - Cell volume is regulated by a delicate balance between ion distribution across the plasma membrane and the osmotic properties of intra- and extracellular components. Using a fluorescent calcein indicator, we analysed the effects of glycosaminoglycans on the cell volume of hyaluronan producing fibroblasts and hyaluronan deficient HEK cells over a time period of 30?h. Exogenous glycosaminoglycans induced cell blebbing after 2?min and swelling of fibroblasts to about 110% of untreated cell volume at low concentrations which decreased at higher concentrations. HEK cells did not show cell blebbing and responded by shrinking to 65% of untreated cell volume. Heparin induced swelling of both fibroblasts and HEK cells. Hyaluronidase treatment or inhibition of hyaluronan export led to cell shrinkage indicating that the hyaluronan coat maintained fibroblasts in a swollen state. These observations were explained by the combined action of the Donnan effect and molecular crowding.
AB - Cell volume is regulated by a delicate balance between ion distribution across the plasma membrane and the osmotic properties of intra- and extracellular components. Using a fluorescent calcein indicator, we analysed the effects of glycosaminoglycans on the cell volume of hyaluronan producing fibroblasts and hyaluronan deficient HEK cells over a time period of 30?h. Exogenous glycosaminoglycans induced cell blebbing after 2?min and swelling of fibroblasts to about 110% of untreated cell volume at low concentrations which decreased at higher concentrations. HEK cells did not show cell blebbing and responded by shrinking to 65% of untreated cell volume. Heparin induced swelling of both fibroblasts and HEK cells. Hyaluronidase treatment or inhibition of hyaluronan export led to cell shrinkage indicating that the hyaluronan coat maintained fibroblasts in a swollen state. These observations were explained by the combined action of the Donnan effect and molecular crowding.
KW - Humans
KW - Cell Line, Tumor
KW - Signal Transduction
KW - HEK293 Cells
KW - Cell Membrane/metabolism
KW - Cell Size/drug effects
KW - Chondroitin Sulfates/pharmacology
KW - Extracellular Matrix
KW - Fibroblasts/physiology
KW - Glycosaminoglycans/metabolism/pharmacology
KW - Heparin/pharmacology
KW - Hyaluronic Acid/biosynthesis/deficiency/pharmacology
KW - Osmosis
KW - Humans
KW - Cell Line, Tumor
KW - Signal Transduction
KW - HEK293 Cells
KW - Cell Membrane/metabolism
KW - Cell Size/drug effects
KW - Chondroitin Sulfates/pharmacology
KW - Extracellular Matrix
KW - Fibroblasts/physiology
KW - Glycosaminoglycans/metabolism/pharmacology
KW - Heparin/pharmacology
KW - Hyaluronic Acid/biosynthesis/deficiency/pharmacology
KW - Osmosis
M3 - SCORING: Journal article
VL - 113
SP - 340
EP - 348
JO - J CELL BIOCHEM
JF - J CELL BIOCHEM
SN - 0730-2312
IS - 1
M1 - 1
ER -
