PortalPublikationenRegulation of brain-derived neurotrophic factor mRNA levels ...

Regulation of brain-derived neurotrophic factor mRNA levels in hippocampus by neuronal activity

Standard

Regulation of brain-derived neurotrophic factor mRNA levels in hippocampus by neuronal activity. / Castrén, E; Berninger, B; Leingärtner, A; Lindholm, D.

in: PROG BRAIN RES, Jahrgang 117, 01.01.1998, S. 57-64.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Castrén, E, Berninger, B, Leingärtner, A & Lindholm, D 1998, 'Regulation of brain-derived neurotrophic factor mRNA levels in hippocampus by neuronal activity', PROG BRAIN RES, Jg. 117, S. 57-64.

APA

Castrén, E., Berninger, B., Leingärtner, A., & Lindholm, D. (1998). Regulation of brain-derived neurotrophic factor mRNA levels in hippocampus by neuronal activity. PROG BRAIN RES, 117, 57-64.

Vancouver

Castrén E, Berninger B, Leingärtner A, Lindholm D. Regulation of brain-derived neurotrophic factor mRNA levels in hippocampus by neuronal activity. PROG BRAIN RES. 1998 Jan 1;117:57-64.

Bibtex

@article{6698145076c9445f87e98616e69d1cad,
title = "Regulation of brain-derived neurotrophic factor mRNA levels in hippocampus by neuronal activity",
abstract = "Neuronal activity increases synthesis of brain-derived neurotrophic factor (BDNF) mRNA in vivo and in vitro. We have investigated the pathways through which neuronal activity stimulated by kainic acid regulates BDNF mRNA levels in cultured hippocampal neurons and transgenic mice. Kainic acid induced the transcription of BDNF mRNA without influencing the mRNA stability. Interestingly, the half-life of the 4.2 kb BDNF transcript was much shorter than that of the 1.6 kb transcript (23 +/- 4 min. vs. 132 +/- 30 min). Increase in the BDNF mRNA levels by kainic acid was not blocked by the protein synthesis inhibitor cycloheximide demonstrating that BDNF is regulated as an immediate early gene in hippocampal neurons. Although calmodulin antagonists are known to abolish the effect of kainic acid on BDNF mRNA, this effect was very similar in Ca(+2)-calmodulin-dependent protein kinase II alpha knock-out mice and in wild-type mice. Surprisingly, even high doses of kainic acid failed to increase nerve growth factor (NGF) mRNA in mouse hippocampus although elevation in rat brain has been consistently observed.",
keywords = "Animals, Brain-Derived Neurotrophic Factor, Calcium-Calmodulin-Dependent Protein Kinase Type 2, Calcium-Calmodulin-Dependent Protein Kinases, Genes, Immediate-Early, Hippocampus, Kainic Acid, Mice, Mice, Knockout, Nerve Growth Factors, Neurons, RNA, Messenger, Rats, Transcription, Genetic",
author = "E Castr{\'e}n and B Berninger and A Leing{\"a}rtner and D Lindholm",
year = "1998",
month = jan,
day = "1",
language = "English",
volume = "117",
pages = "57--64",
journal = "PROG BRAIN RES",
issn = "0079-6123",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Regulation of brain-derived neurotrophic factor mRNA levels in hippocampus by neuronal activity

AU - Castrén, E

AU - Berninger, B

AU - Leingärtner, A

AU - Lindholm, D

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Neuronal activity increases synthesis of brain-derived neurotrophic factor (BDNF) mRNA in vivo and in vitro. We have investigated the pathways through which neuronal activity stimulated by kainic acid regulates BDNF mRNA levels in cultured hippocampal neurons and transgenic mice. Kainic acid induced the transcription of BDNF mRNA without influencing the mRNA stability. Interestingly, the half-life of the 4.2 kb BDNF transcript was much shorter than that of the 1.6 kb transcript (23 +/- 4 min. vs. 132 +/- 30 min). Increase in the BDNF mRNA levels by kainic acid was not blocked by the protein synthesis inhibitor cycloheximide demonstrating that BDNF is regulated as an immediate early gene in hippocampal neurons. Although calmodulin antagonists are known to abolish the effect of kainic acid on BDNF mRNA, this effect was very similar in Ca(+2)-calmodulin-dependent protein kinase II alpha knock-out mice and in wild-type mice. Surprisingly, even high doses of kainic acid failed to increase nerve growth factor (NGF) mRNA in mouse hippocampus although elevation in rat brain has been consistently observed.

AB - Neuronal activity increases synthesis of brain-derived neurotrophic factor (BDNF) mRNA in vivo and in vitro. We have investigated the pathways through which neuronal activity stimulated by kainic acid regulates BDNF mRNA levels in cultured hippocampal neurons and transgenic mice. Kainic acid induced the transcription of BDNF mRNA without influencing the mRNA stability. Interestingly, the half-life of the 4.2 kb BDNF transcript was much shorter than that of the 1.6 kb transcript (23 +/- 4 min. vs. 132 +/- 30 min). Increase in the BDNF mRNA levels by kainic acid was not blocked by the protein synthesis inhibitor cycloheximide demonstrating that BDNF is regulated as an immediate early gene in hippocampal neurons. Although calmodulin antagonists are known to abolish the effect of kainic acid on BDNF mRNA, this effect was very similar in Ca(+2)-calmodulin-dependent protein kinase II alpha knock-out mice and in wild-type mice. Surprisingly, even high doses of kainic acid failed to increase nerve growth factor (NGF) mRNA in mouse hippocampus although elevation in rat brain has been consistently observed.

KW - Animals

KW - Brain-Derived Neurotrophic Factor

KW - Calcium-Calmodulin-Dependent Protein Kinase Type 2

KW - Calcium-Calmodulin-Dependent Protein Kinases

KW - Genes, Immediate-Early

KW - Hippocampus

KW - Kainic Acid

KW - Mice

KW - Mice, Knockout

KW - Nerve Growth Factors

KW - Neurons

KW - RNA, Messenger

KW - Rats

KW - Transcription, Genetic

M3 - SCORING: Journal article

C2 - 9932400

VL - 117

SP - 57

EP - 64

JO - PROG BRAIN RES

JF - PROG BRAIN RES

SN - 0079-6123

ER -