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Regulation of APD and Force by the Na+/Ca2+ Exchanger in Human-Induced Pluripotent Stem Cell-Derived Engineered Heart Tissue

Standard

Regulation of APD and Force by the Na+/Ca2+ Exchanger in Human-Induced Pluripotent Stem Cell-Derived Engineered Heart Tissue. / Ismaili, Djemail; Gurr, Katrin; Horváth, András; Yuan, Lei; Lemoine, Marc D; Schulz, Carl; Sani, Jascha; Petersen, Johannes; Reichenspurner, Hermann; Kirchhof, Paulus; Jespersen, Thomas; Eschenhagen, Thomas; Hansen, Arne; Koivumäki, Jussi T; Christ, Torsten.

in: CELLS-BASEL, Jahrgang 11, Nr. 15, 2424, 05.08.2022.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Ismaili, D, Gurr, K, Horváth, A, Yuan, L, Lemoine, MD, Schulz, C, Sani, J, Petersen, J, Reichenspurner, H, Kirchhof, P, Jespersen, T, Eschenhagen, T, Hansen, A, Koivumäki, JT & Christ, T 2022, 'Regulation of APD and Force by the Na+/Ca2+ Exchanger in Human-Induced Pluripotent Stem Cell-Derived Engineered Heart Tissue', CELLS-BASEL, Jg. 11, Nr. 15, 2424. https://doi.org/10.3390/cells11152424

APA

Ismaili, D., Gurr, K., Horváth, A., Yuan, L., Lemoine, M. D., Schulz, C., Sani, J., Petersen, J., Reichenspurner, H., Kirchhof, P., Jespersen, T., Eschenhagen, T., Hansen, A., Koivumäki, J. T., & Christ, T. (2022). Regulation of APD and Force by the Na+/Ca2+ Exchanger in Human-Induced Pluripotent Stem Cell-Derived Engineered Heart Tissue. CELLS-BASEL, 11(15), [2424]. https://doi.org/10.3390/cells11152424

Vancouver

Ismaili D, Gurr K, Horváth A, Yuan L, Lemoine MD, Schulz C et al. Regulation of APD and Force by the Na+/Ca2+ Exchanger in Human-Induced Pluripotent Stem Cell-Derived Engineered Heart Tissue. CELLS-BASEL. 2022 Aug 5;11(15). 2424. https://doi.org/10.3390/cells11152424

Bibtex

@article{fd5742a6d13b43cbb40f123bdaa294a0,
title = "Regulation of APD and Force by the Na+/Ca2+ Exchanger in Human-Induced Pluripotent Stem Cell-Derived Engineered Heart Tissue",
abstract = "The physiological importance of NCX in human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) is not well characterized but may depend on the relative strength of the current, compared to adult cardiomyocytes, and on the exact spatial arrangement of proteins involved in Ca2+ extrusion. Here, we determined NCX currents and its contribution to action potential and force in hiPSC-CMs cultured in engineered heart tissue (EHT). The results were compared with data from rat and human left ventricular tissue. The NCX currents in hiPSC-CMs were larger than in ventricular cardiomyocytes isolated from human left ventricles (1.3 ± 0.2 pA/pF and 3.2 ± 0.2 pA/pF for human ventricle and EHT, respectively, p < 0.05). SEA0400 (10 µM) markedly shortened the APD90 in EHT (by 26.6 ± 5%, p < 0.05) and, to a lesser extent, in rat ventricular tissue (by 10.7 ± 1.6%, p < 0.05). Shortening in human left ventricular preparations was small and not different from time-matched controls (TMCs; p > 0.05). Force was increased by the NCX block in rat ventricle (by 31 ± 5.4%, p < 0.05) and EHT (by 20.8 ± 3.9%, p < 0.05), but not in human left ventricular preparations. In conclusion, hiPSC-CMs possess NCX currents not smaller than human left ventricular tissue. Robust NCX block-induced APD shortening and inotropy makes EHT an attractive pharmacological model.",
keywords = "Action Potentials, Adult, Animals, Heart Ventricles/metabolism, Humans, Induced Pluripotent Stem Cells/metabolism, Myocytes, Cardiac/metabolism, Rats, Sodium-Calcium Exchanger/metabolism",
author = "Djemail Ismaili and Katrin Gurr and Andr{\'a}s Horv{\'a}th and Lei Yuan and Lemoine, {Marc D} and Carl Schulz and Jascha Sani and Johannes Petersen and Hermann Reichenspurner and Paulus Kirchhof and Thomas Jespersen and Thomas Eschenhagen and Arne Hansen and Koivum{\"a}ki, {Jussi T} and Torsten Christ",
year = "2022",
month = aug,
day = "5",
doi = "10.3390/cells11152424",
language = "English",
volume = "11",
journal = "CELLS-BASEL",
issn = "2073-4409",
publisher = "MDPI Multidisciplinary Digital Publishing Institute",
number = "15",

}

RIS

TY - JOUR

T1 - Regulation of APD and Force by the Na+/Ca2+ Exchanger in Human-Induced Pluripotent Stem Cell-Derived Engineered Heart Tissue

AU - Ismaili, Djemail

AU - Gurr, Katrin

AU - Horváth, András

AU - Yuan, Lei

AU - Lemoine, Marc D

AU - Schulz, Carl

AU - Sani, Jascha

AU - Petersen, Johannes

AU - Reichenspurner, Hermann

AU - Kirchhof, Paulus

AU - Jespersen, Thomas

AU - Eschenhagen, Thomas

AU - Hansen, Arne

AU - Koivumäki, Jussi T

AU - Christ, Torsten

PY - 2022/8/5

Y1 - 2022/8/5

N2 - The physiological importance of NCX in human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) is not well characterized but may depend on the relative strength of the current, compared to adult cardiomyocytes, and on the exact spatial arrangement of proteins involved in Ca2+ extrusion. Here, we determined NCX currents and its contribution to action potential and force in hiPSC-CMs cultured in engineered heart tissue (EHT). The results were compared with data from rat and human left ventricular tissue. The NCX currents in hiPSC-CMs were larger than in ventricular cardiomyocytes isolated from human left ventricles (1.3 ± 0.2 pA/pF and 3.2 ± 0.2 pA/pF for human ventricle and EHT, respectively, p < 0.05). SEA0400 (10 µM) markedly shortened the APD90 in EHT (by 26.6 ± 5%, p < 0.05) and, to a lesser extent, in rat ventricular tissue (by 10.7 ± 1.6%, p < 0.05). Shortening in human left ventricular preparations was small and not different from time-matched controls (TMCs; p > 0.05). Force was increased by the NCX block in rat ventricle (by 31 ± 5.4%, p < 0.05) and EHT (by 20.8 ± 3.9%, p < 0.05), but not in human left ventricular preparations. In conclusion, hiPSC-CMs possess NCX currents not smaller than human left ventricular tissue. Robust NCX block-induced APD shortening and inotropy makes EHT an attractive pharmacological model.

AB - The physiological importance of NCX in human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) is not well characterized but may depend on the relative strength of the current, compared to adult cardiomyocytes, and on the exact spatial arrangement of proteins involved in Ca2+ extrusion. Here, we determined NCX currents and its contribution to action potential and force in hiPSC-CMs cultured in engineered heart tissue (EHT). The results were compared with data from rat and human left ventricular tissue. The NCX currents in hiPSC-CMs were larger than in ventricular cardiomyocytes isolated from human left ventricles (1.3 ± 0.2 pA/pF and 3.2 ± 0.2 pA/pF for human ventricle and EHT, respectively, p < 0.05). SEA0400 (10 µM) markedly shortened the APD90 in EHT (by 26.6 ± 5%, p < 0.05) and, to a lesser extent, in rat ventricular tissue (by 10.7 ± 1.6%, p < 0.05). Shortening in human left ventricular preparations was small and not different from time-matched controls (TMCs; p > 0.05). Force was increased by the NCX block in rat ventricle (by 31 ± 5.4%, p < 0.05) and EHT (by 20.8 ± 3.9%, p < 0.05), but not in human left ventricular preparations. In conclusion, hiPSC-CMs possess NCX currents not smaller than human left ventricular tissue. Robust NCX block-induced APD shortening and inotropy makes EHT an attractive pharmacological model.

KW - Action Potentials

KW - Adult

KW - Animals

KW - Heart Ventricles/metabolism

KW - Humans

KW - Induced Pluripotent Stem Cells/metabolism

KW - Myocytes, Cardiac/metabolism

KW - Rats

KW - Sodium-Calcium Exchanger/metabolism

U2 - 10.3390/cells11152424

DO - 10.3390/cells11152424

M3 - SCORING: Journal article

C2 - 35954268

VL - 11

JO - CELLS-BASEL

JF - CELLS-BASEL

SN - 2073-4409

IS - 15

M1 - 2424

ER -