Regional and subcellular distribution of the receptor-targeting protein PIST in the rat central nervous system.
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Regional and subcellular distribution of the receptor-targeting protein PIST in the rat central nervous system. / Chen, Annie; Gössling, Enno Karl; Witkowski, Leora; Bhindi, Amar; Bauch, Carola; Roussy, Geneviève; Sarret, Philippe; Kreienkamp, Hans-Jürgen; Stroh, Thomas.
in: J COMP NEUROL, Jahrgang 520, Nr. 5, 5, 2012, S. 889-913.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Regional and subcellular distribution of the receptor-targeting protein PIST in the rat central nervous system.
AU - Chen, Annie
AU - Gössling, Enno Karl
AU - Witkowski, Leora
AU - Bhindi, Amar
AU - Bauch, Carola
AU - Roussy, Geneviève
AU - Sarret, Philippe
AU - Kreienkamp, Hans-Jürgen
AU - Stroh, Thomas
PY - 2012
Y1 - 2012
N2 - Protein interacting specifically with Tc10, PIST, is a Golgi-associated sorting protein involved in regulating cell-surface targeting of plasma membrane receptors. The present study provides the first comprehensive description of PIST distribution in the mammalian central nervous system and of its subcellular localization by immunocytochemistry. PIST is distributed widely throughout the neuraxis, predominantly associated with neuronal cell bodies and dendrites. In hippocampal neurons, in vitro and in situ, PIST displayed a patchy subcellular distribution in an area surrounding the nucleus and extending into one of the major dendrites. By colocalization with the trans-Golgi marker TGN38, we were able to show that PIST is associated largely but not exclusively with the trans-Golgi network in central neurons. High or moderate to high levels of PIST-like immunoreactivity were found in cortical areas, in particular in layer V of the neocortex. The motor cortex was most strongly labeled. Also, the piriform and insular cortices displayed strong PIST labeling. In the hippocampus, CA2 but not CA1 or CA3 pyramidal cells displayed strong PIST-labeling, extending into their apical dendrites. In the thalamus, ventrolateral and laterodorsal nuclei were most strongly stained, whereas in the hypothalamus the supraoptic nucleus stood out with strong immunoreactivity. Strikingly, in the brainstem all cranial nerve motor nuclei were PIST-positive at varying levels, which is in keeping with the prominent expression of PIST in forebrain motor areas. This selective distribution of PIST suggests that the protein serves distinctive roles in specific neuronal populations, establishing functionally distinct zones, for instance, in the hippocampus.
AB - Protein interacting specifically with Tc10, PIST, is a Golgi-associated sorting protein involved in regulating cell-surface targeting of plasma membrane receptors. The present study provides the first comprehensive description of PIST distribution in the mammalian central nervous system and of its subcellular localization by immunocytochemistry. PIST is distributed widely throughout the neuraxis, predominantly associated with neuronal cell bodies and dendrites. In hippocampal neurons, in vitro and in situ, PIST displayed a patchy subcellular distribution in an area surrounding the nucleus and extending into one of the major dendrites. By colocalization with the trans-Golgi marker TGN38, we were able to show that PIST is associated largely but not exclusively with the trans-Golgi network in central neurons. High or moderate to high levels of PIST-like immunoreactivity were found in cortical areas, in particular in layer V of the neocortex. The motor cortex was most strongly labeled. Also, the piriform and insular cortices displayed strong PIST labeling. In the hippocampus, CA2 but not CA1 or CA3 pyramidal cells displayed strong PIST-labeling, extending into their apical dendrites. In the thalamus, ventrolateral and laterodorsal nuclei were most strongly stained, whereas in the hypothalamus the supraoptic nucleus stood out with strong immunoreactivity. Strikingly, in the brainstem all cranial nerve motor nuclei were PIST-positive at varying levels, which is in keeping with the prominent expression of PIST in forebrain motor areas. This selective distribution of PIST suggests that the protein serves distinctive roles in specific neuronal populations, establishing functionally distinct zones, for instance, in the hippocampus.
KW - Animals
KW - Humans
KW - Male
KW - Cells, Cultured
KW - Rats
KW - Rats, Sprague-Dawley
KW - HEK293 Cells
KW - Guinea Pigs
KW - Membrane Glycoproteins/metabolism
KW - Receptors, Cell Surface/metabolism
KW - Carrier Proteins/chemistry/metabolism
KW - Central Nervous System/chemistry/metabolism
KW - Hippocampus/chemistry/metabolism
KW - Nerve Tissue Proteins/chemistry/metabolism
KW - Subcellular Fractions/chemistry/metabolism
KW - trans-Golgi Network/chemistry/metabolism
KW - Animals
KW - Humans
KW - Male
KW - Cells, Cultured
KW - Rats
KW - Rats, Sprague-Dawley
KW - HEK293 Cells
KW - Guinea Pigs
KW - Membrane Glycoproteins/metabolism
KW - Receptors, Cell Surface/metabolism
KW - Carrier Proteins/chemistry/metabolism
KW - Central Nervous System/chemistry/metabolism
KW - Hippocampus/chemistry/metabolism
KW - Nerve Tissue Proteins/chemistry/metabolism
KW - Subcellular Fractions/chemistry/metabolism
KW - trans-Golgi Network/chemistry/metabolism
M3 - SCORING: Journal article
VL - 520
SP - 889
EP - 913
JO - J COMP NEUROL
JF - J COMP NEUROL
SN - 0021-9967
IS - 5
M1 - 5
ER -
