Reelin together with ApoER2 regulates interneuron migration in the olfactory bulb.
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Reelin together with ApoER2 regulates interneuron migration in the olfactory bulb. / Hellwig, Sabine; Hack, Iris; Zucker, Birgit; Brunne, Bianca; Junghans, Dirk.
in: PLOS ONE, Jahrgang 7, Nr. 11, 11, 2012, S. 50646.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Reelin together with ApoER2 regulates interneuron migration in the olfactory bulb.
AU - Hellwig, Sabine
AU - Hack, Iris
AU - Zucker, Birgit
AU - Brunne, Bianca
AU - Junghans, Dirk
PY - 2012
Y1 - 2012
N2 - One pathway regulating the migration of neurons during development of the mammalian cortex involves the extracellular matrix protein Reelin. Reelin and components of its signaling cascade, the lipoprotein receptors ApoER2 and Vldlr and the intracellular adapter protein Dab1 are pivotal for a correct layer formation during corticogenesis. The olfactory bulb (OB) as a phylogenetically old cortical region is known to be a prominent site of Reelin expression. Although some aspects of Reelin function in the OB have been described, the influence of Reelin on OB layer formation has so far been poorly analyzed. Here we studied animals deficient for either Reelin, Vldlr, ApoER2 or Dab1 as well as double-null mutants. We performed organotypic migration assays, immunohistochemical marker analysis and BrdU incorporation studies to elucidate roles for the different components of the Reelin signaling cascade in OB neuroblast migration and layer formation. We identified ApoER2 as being the main receptor responsible for Reelin mediated detachment of neuroblasts and correct migration of early generated interneurons within the OB, a prerequisite for correct OB lamination.
AB - One pathway regulating the migration of neurons during development of the mammalian cortex involves the extracellular matrix protein Reelin. Reelin and components of its signaling cascade, the lipoprotein receptors ApoER2 and Vldlr and the intracellular adapter protein Dab1 are pivotal for a correct layer formation during corticogenesis. The olfactory bulb (OB) as a phylogenetically old cortical region is known to be a prominent site of Reelin expression. Although some aspects of Reelin function in the OB have been described, the influence of Reelin on OB layer formation has so far been poorly analyzed. Here we studied animals deficient for either Reelin, Vldlr, ApoER2 or Dab1 as well as double-null mutants. We performed organotypic migration assays, immunohistochemical marker analysis and BrdU incorporation studies to elucidate roles for the different components of the Reelin signaling cascade in OB neuroblast migration and layer formation. We identified ApoER2 as being the main receptor responsible for Reelin mediated detachment of neuroblasts and correct migration of early generated interneurons within the OB, a prerequisite for correct OB lamination.
KW - Animals
KW - Immunohistochemistry
KW - Mice
KW - Mice, Knockout
KW - Immunoblotting
KW - In Situ Hybridization
KW - Real-Time Polymerase Chain Reaction
KW - Nerve Tissue Proteins/genetics/metabolism
KW - Serine Endopeptidases/genetics/metabolism
KW - Cell Adhesion Molecules, Neuronal/genetics/metabolism
KW - Signal Transduction/genetics/physiology
KW - Receptors, LDL/genetics/metabolism
KW - Cell Movement/genetics/physiology
KW - Extracellular Matrix Proteins/genetics/metabolism
KW - LDL-Receptor Related Proteins/genetics/metabolism
KW - Olfactory Bulb/cytology/metabolism
KW - Prosencephalon/metabolism
KW - Animals
KW - Immunohistochemistry
KW - Mice
KW - Mice, Knockout
KW - Immunoblotting
KW - In Situ Hybridization
KW - Real-Time Polymerase Chain Reaction
KW - Nerve Tissue Proteins/genetics/metabolism
KW - Serine Endopeptidases/genetics/metabolism
KW - Cell Adhesion Molecules, Neuronal/genetics/metabolism
KW - Signal Transduction/genetics/physiology
KW - Receptors, LDL/genetics/metabolism
KW - Cell Movement/genetics/physiology
KW - Extracellular Matrix Proteins/genetics/metabolism
KW - LDL-Receptor Related Proteins/genetics/metabolism
KW - Olfactory Bulb/cytology/metabolism
KW - Prosencephalon/metabolism
U2 - 10.1371/journal.pone.0050646
DO - 10.1371/journal.pone.0050646
M3 - SCORING: Journal article
VL - 7
SP - 50646
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 11
M1 - 11
ER -
