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Reduced adhesion of aged intestinal stem cells contributes to an accelerated clonal drift

Standard

Reduced adhesion of aged intestinal stem cells contributes to an accelerated clonal drift. / Hageb, Ali; Thalheim, Torsten; Nattamai, Kalpana J; Möhrle, Bettina; Saçma, Mehmet; Sakk, Vadim; Thielecke, Lars; Kerstin, Cornils; Grandy, Carolin; Port, Fabian; Gottschalk, Kay-E; Mallm, Jan-Philipp; Glauche, Ingmar; Galle, Jörg; Mulaw, Medhanie A; Geiger, Hartmut.

in: LIFE SCI ALLIANCE, Jahrgang 5, Nr. 8, e202201408, 08.2022.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Hageb, A, Thalheim, T, Nattamai, KJ, Möhrle, B, Saçma, M, Sakk, V, Thielecke, L, Kerstin, C, Grandy, C, Port, F, Gottschalk, K-E, Mallm, J-P, Glauche, I, Galle, J, Mulaw, MA & Geiger, H 2022, 'Reduced adhesion of aged intestinal stem cells contributes to an accelerated clonal drift', LIFE SCI ALLIANCE, Jg. 5, Nr. 8, e202201408. https://doi.org/10.26508/lsa.202201408

APA

Hageb, A., Thalheim, T., Nattamai, K. J., Möhrle, B., Saçma, M., Sakk, V., Thielecke, L., Kerstin, C., Grandy, C., Port, F., Gottschalk, K-E., Mallm, J-P., Glauche, I., Galle, J., Mulaw, M. A., & Geiger, H. (2022). Reduced adhesion of aged intestinal stem cells contributes to an accelerated clonal drift. LIFE SCI ALLIANCE, 5(8), [e202201408]. https://doi.org/10.26508/lsa.202201408

Vancouver

Hageb A, Thalheim T, Nattamai KJ, Möhrle B, Saçma M, Sakk V et al. Reduced adhesion of aged intestinal stem cells contributes to an accelerated clonal drift. LIFE SCI ALLIANCE. 2022 Aug;5(8). e202201408. https://doi.org/10.26508/lsa.202201408

Bibtex

@article{af18c878209443a3995cc858e6125516,
title = "Reduced adhesion of aged intestinal stem cells contributes to an accelerated clonal drift",
abstract = "Upon aging, the function of the intestinal epithelium declines with a concomitant increase in aging-related diseases. ISCs play an important role in this process. It is known that ISC clonal dynamics follow a neutral drift model. However, it is not clear whether the drift model is still valid in aged ISCs. Tracking of clonal dynamics by clonal tracing revealed that aged crypts drift into monoclonality substantially faster than young ones. However, ISC tracing experiments, in vivo and ex vivo, implied a similar clonal expansion ability of both young and aged ISCs. Single-cell RNA sequencing for 1,920 high Lgr5 ISCs from young and aged mice revealed increased heterogeneity among subgroups of aged ISCs. Genes associated with cell adhesion were down-regulated in aged ISCs. ISCs of aged mice indeed show weaker adhesion to the matrix. Simulations applying a single cell-based model of the small intestinal crypt demonstrated an accelerated clonal drift at reduced adhesion strength, implying a central role for reduced adhesion for affecting clonal dynamics upon aging.",
keywords = "Animals, Cells, Cultured, Ileum, Intestinal Mucosa/metabolism, Intestines, Mice, Stem Cells/metabolism",
author = "Ali Hageb and Torsten Thalheim and Nattamai, {Kalpana J} and Bettina M{\"o}hrle and Mehmet Sa{\c c}ma and Vadim Sakk and Lars Thielecke and Cornils Kerstin and Carolin Grandy and Fabian Port and Kay-E Gottschalk and Jan-Philipp Mallm and Ingmar Glauche and J{\"o}rg Galle and Mulaw, {Medhanie A} and Hartmut Geiger",
note = "{\textcopyright} 2022 Hageb et al.",
year = "2022",
month = aug,
doi = "10.26508/lsa.202201408",
language = "English",
volume = "5",
journal = "LIFE SCI ALLIANCE",
issn = "2575-1077",
publisher = "Rockefeller University Press",
number = "8",

}

RIS

TY - JOUR

T1 - Reduced adhesion of aged intestinal stem cells contributes to an accelerated clonal drift

AU - Hageb, Ali

AU - Thalheim, Torsten

AU - Nattamai, Kalpana J

AU - Möhrle, Bettina

AU - Saçma, Mehmet

AU - Sakk, Vadim

AU - Thielecke, Lars

AU - Kerstin, Cornils

AU - Grandy, Carolin

AU - Port, Fabian

AU - Gottschalk, Kay-E

AU - Mallm, Jan-Philipp

AU - Glauche, Ingmar

AU - Galle, Jörg

AU - Mulaw, Medhanie A

AU - Geiger, Hartmut

N1 - © 2022 Hageb et al.

PY - 2022/8

Y1 - 2022/8

N2 - Upon aging, the function of the intestinal epithelium declines with a concomitant increase in aging-related diseases. ISCs play an important role in this process. It is known that ISC clonal dynamics follow a neutral drift model. However, it is not clear whether the drift model is still valid in aged ISCs. Tracking of clonal dynamics by clonal tracing revealed that aged crypts drift into monoclonality substantially faster than young ones. However, ISC tracing experiments, in vivo and ex vivo, implied a similar clonal expansion ability of both young and aged ISCs. Single-cell RNA sequencing for 1,920 high Lgr5 ISCs from young and aged mice revealed increased heterogeneity among subgroups of aged ISCs. Genes associated with cell adhesion were down-regulated in aged ISCs. ISCs of aged mice indeed show weaker adhesion to the matrix. Simulations applying a single cell-based model of the small intestinal crypt demonstrated an accelerated clonal drift at reduced adhesion strength, implying a central role for reduced adhesion for affecting clonal dynamics upon aging.

AB - Upon aging, the function of the intestinal epithelium declines with a concomitant increase in aging-related diseases. ISCs play an important role in this process. It is known that ISC clonal dynamics follow a neutral drift model. However, it is not clear whether the drift model is still valid in aged ISCs. Tracking of clonal dynamics by clonal tracing revealed that aged crypts drift into monoclonality substantially faster than young ones. However, ISC tracing experiments, in vivo and ex vivo, implied a similar clonal expansion ability of both young and aged ISCs. Single-cell RNA sequencing for 1,920 high Lgr5 ISCs from young and aged mice revealed increased heterogeneity among subgroups of aged ISCs. Genes associated with cell adhesion were down-regulated in aged ISCs. ISCs of aged mice indeed show weaker adhesion to the matrix. Simulations applying a single cell-based model of the small intestinal crypt demonstrated an accelerated clonal drift at reduced adhesion strength, implying a central role for reduced adhesion for affecting clonal dynamics upon aging.

KW - Animals

KW - Cells, Cultured

KW - Ileum

KW - Intestinal Mucosa/metabolism

KW - Intestines

KW - Mice

KW - Stem Cells/metabolism

U2 - 10.26508/lsa.202201408

DO - 10.26508/lsa.202201408

M3 - SCORING: Journal article

C2 - 35487692

VL - 5

JO - LIFE SCI ALLIANCE

JF - LIFE SCI ALLIANCE

SN - 2575-1077

IS - 8

M1 - e202201408

ER -