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Rat platelets contain growth factor(s) distinct from PDGF which stimulate DNA synthesis in primary adult rat hepatocyte cultures.

Standard

Rat platelets contain growth factor(s) distinct from PDGF which stimulate DNA synthesis in primary adult rat hepatocyte cultures. / Paul, D; Piasecki, Angelika.

in: EXP CELL RES, Jahrgang 154, Nr. 1, 1, 1984, S. 95-100.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Paul, D & Piasecki, A 1984, 'Rat platelets contain growth factor(s) distinct from PDGF which stimulate DNA synthesis in primary adult rat hepatocyte cultures.', EXP CELL RES, Jg. 154, Nr. 1, 1, S. 95-100. <http://www.ncbi.nlm.nih.gov/pubmed/6468536?dopt=Citation>

APA

Paul, D., & Piasecki, A. (1984). Rat platelets contain growth factor(s) distinct from PDGF which stimulate DNA synthesis in primary adult rat hepatocyte cultures. EXP CELL RES, 154(1), 95-100. [1]. http://www.ncbi.nlm.nih.gov/pubmed/6468536?dopt=Citation

Vancouver

Paul D, Piasecki A. Rat platelets contain growth factor(s) distinct from PDGF which stimulate DNA synthesis in primary adult rat hepatocyte cultures. EXP CELL RES. 1984;154(1):95-100. 1.

Bibtex

@article{c311b82b6651490aa87fc70599cff35f,
title = "Rat platelets contain growth factor(s) distinct from PDGF which stimulate DNA synthesis in primary adult rat hepatocyte cultures.",
abstract = "Adult rat hepatocytes in primary cultures are stimulated to synthesize DNA in response to rat serum, whereas rat plasma is considerably less active. Biological activity is present in rat platelets and is secreted during aggregation in response to thrombin. The material secreted by rat platelets is heat labile and is sensitive to digestion with trypsin, suggesting that it is a protein. When assayed on 3T3 cells this material also stimulates DNA synthesis; however, the trypsin-sensitive activity is heat stable (100 degrees C, 10 min). These results indicate that rat platelets contain hepatotrophic activities which by virtue of their heat sensitivity are distinct from heat-stable platelet-derived growth factor (PDGF)-like mitogenic activities required by 3T3 cells for growth. It is possible that hepatotrophic platelet factors might be involved in mediating liver regeneration in the rat after partial hepatectomy.",
keywords = "Animals, Cells, Cultured, Mice, Rats, Rats, Inbred Strains, Blood Platelets/*physiology, *DNA Replication, Growth Substances/blood/isolation & purification/*physiology, Liver/*metabolism, Platelet-Derived Growth Factor/physiology, Trypsin, Animals, Cells, Cultured, Mice, Rats, Rats, Inbred Strains, Blood Platelets/*physiology, *DNA Replication, Growth Substances/blood/isolation & purification/*physiology, Liver/*metabolism, Platelet-Derived Growth Factor/physiology, Trypsin",
author = "D Paul and Angelika Piasecki",
year = "1984",
language = "English",
volume = "154",
pages = "95--100",
journal = "EXP CELL RES",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "1",

}

RIS

TY - JOUR

T1 - Rat platelets contain growth factor(s) distinct from PDGF which stimulate DNA synthesis in primary adult rat hepatocyte cultures.

AU - Paul, D

AU - Piasecki, Angelika

PY - 1984

Y1 - 1984

N2 - Adult rat hepatocytes in primary cultures are stimulated to synthesize DNA in response to rat serum, whereas rat plasma is considerably less active. Biological activity is present in rat platelets and is secreted during aggregation in response to thrombin. The material secreted by rat platelets is heat labile and is sensitive to digestion with trypsin, suggesting that it is a protein. When assayed on 3T3 cells this material also stimulates DNA synthesis; however, the trypsin-sensitive activity is heat stable (100 degrees C, 10 min). These results indicate that rat platelets contain hepatotrophic activities which by virtue of their heat sensitivity are distinct from heat-stable platelet-derived growth factor (PDGF)-like mitogenic activities required by 3T3 cells for growth. It is possible that hepatotrophic platelet factors might be involved in mediating liver regeneration in the rat after partial hepatectomy.

AB - Adult rat hepatocytes in primary cultures are stimulated to synthesize DNA in response to rat serum, whereas rat plasma is considerably less active. Biological activity is present in rat platelets and is secreted during aggregation in response to thrombin. The material secreted by rat platelets is heat labile and is sensitive to digestion with trypsin, suggesting that it is a protein. When assayed on 3T3 cells this material also stimulates DNA synthesis; however, the trypsin-sensitive activity is heat stable (100 degrees C, 10 min). These results indicate that rat platelets contain hepatotrophic activities which by virtue of their heat sensitivity are distinct from heat-stable platelet-derived growth factor (PDGF)-like mitogenic activities required by 3T3 cells for growth. It is possible that hepatotrophic platelet factors might be involved in mediating liver regeneration in the rat after partial hepatectomy.

KW - Animals

KW - Cells, Cultured

KW - Mice

KW - Rats

KW - Rats, Inbred Strains

KW - Blood Platelets/physiology

KW - DNA Replication

KW - Growth Substances/blood/isolation & purification/physiology

KW - Liver/metabolism

KW - Platelet-Derived Growth Factor/physiology

KW - Trypsin

KW - Animals

KW - Cells, Cultured

KW - Mice

KW - Rats

KW - Rats, Inbred Strains

KW - Blood Platelets/physiology

KW - DNA Replication

KW - Growth Substances/blood/isolation & purification/physiology

KW - Liver/metabolism

KW - Platelet-Derived Growth Factor/physiology

KW - Trypsin

M3 - SCORING: Journal article

VL - 154

SP - 95

EP - 100

JO - EXP CELL RES

JF - EXP CELL RES

SN - 0014-4827

IS - 1

M1 - 1

ER -