Rapid development of severe end-organ damage in C57BL/6 mice by combining DOCA salt and angiotensin II.
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Rapid development of severe end-organ damage in C57BL/6 mice by combining DOCA salt and angiotensin II. / Kirchhoff, F; Krebs, Christian; Abdulhag, U N; Meyer-Schwesinger, Catherine; Maas, Renke; Helmchen, Udo; Hilgers, K F; Wolf, Gunter; Stahl, Rolf A.K.; Wenzel, Ulrich.
in: KIDNEY INT, Jahrgang 73, Nr. 5, 5, 2008, S. 643-650.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Rapid development of severe end-organ damage in C57BL/6 mice by combining DOCA salt and angiotensin II.
AU - Kirchhoff, F
AU - Krebs, Christian
AU - Abdulhag, U N
AU - Meyer-Schwesinger, Catherine
AU - Maas, Renke
AU - Helmchen, Udo
AU - Hilgers, K F
AU - Wolf, Gunter
AU - Stahl, Rolf A.K.
AU - Wenzel, Ulrich
PY - 2008
Y1 - 2008
N2 - The C57BL/6 mouse strain serves as the genetic background of many transgenic and gene knockout models; however, this strain appears to be resistant to hypertension-induced renal injury. We developed a new model of hypertensive end-organ damage in C57BL/6 mice by combining deoxycorticosterone acetate (DOCA) and salt with angiotensin II infusion. The systolic blood pressure (SBP) was significantly elevated in DOCA salt-angiotensin II mice compared to control mice or mice treated individually with DOCA salt or angiotensin II. Hypertensive glomerular damage, increased expression of profibrotic and inflammatory genes, albuminuria, tubular casts, increased plasma cholesterol, cardiac hypertrophy, and fibrosis were found in mice treated with DOCA salt-angiotensin II. The SBP in the angiotensin II-infused group was further increased by increasing the infusion rate; only mild injury was observed in these mice, suggesting that blood pressure was not a causal factor. Removal of DOCA and the angiotensin pump lowered blood pressure to normal; however, albuminuria along with the glomerular and cardiac damage did not completely resolve. Our study describes a new model of hypertensive end-organ damage and repair in C57BL/6 mice.
AB - The C57BL/6 mouse strain serves as the genetic background of many transgenic and gene knockout models; however, this strain appears to be resistant to hypertension-induced renal injury. We developed a new model of hypertensive end-organ damage in C57BL/6 mice by combining deoxycorticosterone acetate (DOCA) and salt with angiotensin II infusion. The systolic blood pressure (SBP) was significantly elevated in DOCA salt-angiotensin II mice compared to control mice or mice treated individually with DOCA salt or angiotensin II. Hypertensive glomerular damage, increased expression of profibrotic and inflammatory genes, albuminuria, tubular casts, increased plasma cholesterol, cardiac hypertrophy, and fibrosis were found in mice treated with DOCA salt-angiotensin II. The SBP in the angiotensin II-infused group was further increased by increasing the infusion rate; only mild injury was observed in these mice, suggesting that blood pressure was not a causal factor. Removal of DOCA and the angiotensin pump lowered blood pressure to normal; however, albuminuria along with the glomerular and cardiac damage did not completely resolve. Our study describes a new model of hypertensive end-organ damage and repair in C57BL/6 mice.
M3 - SCORING: Zeitschriftenaufsatz
VL - 73
SP - 643
EP - 650
JO - KIDNEY INT
JF - KIDNEY INT
SN - 0085-2538
IS - 5
M1 - 5
ER -
