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Rapid and efficient cloning of proviral flanking fragments by kanamycin resistance gene complementation

Standard

Rapid and efficient cloning of proviral flanking fragments by kanamycin resistance gene complementation. / Fehse, B; Kühlcke, K; Langer, A; Ostertag, W; Lother, H.

in: NUCLEIC ACIDS RES, Jahrgang 27, Nr. 2, 15.01.1999, S. 706-7.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Fehse, B, Kühlcke, K, Langer, A, Ostertag, W & Lother, H 1999, 'Rapid and efficient cloning of proviral flanking fragments by kanamycin resistance gene complementation', NUCLEIC ACIDS RES, Jg. 27, Nr. 2, S. 706-7.

APA

Fehse, B., Kühlcke, K., Langer, A., Ostertag, W., & Lother, H. (1999). Rapid and efficient cloning of proviral flanking fragments by kanamycin resistance gene complementation. NUCLEIC ACIDS RES, 27(2), 706-7.

Vancouver

Fehse B, Kühlcke K, Langer A, Ostertag W, Lother H. Rapid and efficient cloning of proviral flanking fragments by kanamycin resistance gene complementation. NUCLEIC ACIDS RES. 1999 Jan 15;27(2):706-7.

Bibtex

@article{3305d82f308d434887bd5e7d8ccada5e,
title = "Rapid and efficient cloning of proviral flanking fragments by kanamycin resistance gene complementation",
abstract = "We have developed a technique for the rapid cloning of unknown flanking regions of transgenic DNA. We complemented a truncated kanamycin resistance gene of a bacterial plasmid with a neomycin resistance gene fragment from a gene transfer vector. Optimized transformation conditions allowed us to directly select for kanamycin-resistant bacteria. We cloned numerous proviral flanking fragments from growth factor-independent cell mutants that were obtained after infection with a replication incompetent retroviral vector and identified integrations into the cyclin D2 and several unknown genomic sequences. We anticipate that our method could be adapted to various vector systems that are used to tag and identify genes and to map genomes.",
keywords = "Cloning, Molecular, Cyclin D2, Cyclins, Genetic Complementation Test, Hematopoietic Stem Cells, Humans, Kanamycin Resistance, Proviruses, Retroviridae",
author = "B Fehse and K K{\"u}hlcke and A Langer and W Ostertag and H Lother",
year = "1999",
month = jan,
day = "15",
language = "English",
volume = "27",
pages = "706--7",
journal = "NUCLEIC ACIDS RES",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "2",

}

RIS

TY - JOUR

T1 - Rapid and efficient cloning of proviral flanking fragments by kanamycin resistance gene complementation

AU - Fehse, B

AU - Kühlcke, K

AU - Langer, A

AU - Ostertag, W

AU - Lother, H

PY - 1999/1/15

Y1 - 1999/1/15

N2 - We have developed a technique for the rapid cloning of unknown flanking regions of transgenic DNA. We complemented a truncated kanamycin resistance gene of a bacterial plasmid with a neomycin resistance gene fragment from a gene transfer vector. Optimized transformation conditions allowed us to directly select for kanamycin-resistant bacteria. We cloned numerous proviral flanking fragments from growth factor-independent cell mutants that were obtained after infection with a replication incompetent retroviral vector and identified integrations into the cyclin D2 and several unknown genomic sequences. We anticipate that our method could be adapted to various vector systems that are used to tag and identify genes and to map genomes.

AB - We have developed a technique for the rapid cloning of unknown flanking regions of transgenic DNA. We complemented a truncated kanamycin resistance gene of a bacterial plasmid with a neomycin resistance gene fragment from a gene transfer vector. Optimized transformation conditions allowed us to directly select for kanamycin-resistant bacteria. We cloned numerous proviral flanking fragments from growth factor-independent cell mutants that were obtained after infection with a replication incompetent retroviral vector and identified integrations into the cyclin D2 and several unknown genomic sequences. We anticipate that our method could be adapted to various vector systems that are used to tag and identify genes and to map genomes.

KW - Cloning, Molecular

KW - Cyclin D2

KW - Cyclins

KW - Genetic Complementation Test

KW - Hematopoietic Stem Cells

KW - Humans

KW - Kanamycin Resistance

KW - Proviruses

KW - Retroviridae

M3 - SCORING: Journal article

C2 - 9863001

VL - 27

SP - 706

EP - 707

JO - NUCLEIC ACIDS RES

JF - NUCLEIC ACIDS RES

SN - 0305-1048

IS - 2

ER -