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Radiation-induced transgenerational alterations in genome stability and DNA damage

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Radiation-induced transgenerational alterations in genome stability and DNA damage. / Barber, R C; Hickenbotham, P; Hatch, T; Kelly, D; Topchiy, N; Almeida, G M; Jones, G D D; Johnson, G E; Parry, J M; Rothkamm, K; Dubrova, Y E.

in: ONCOGENE, Jahrgang 25, Nr. 56, 30.11.2006, S. 7336-42.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Barber, RC, Hickenbotham, P, Hatch, T, Kelly, D, Topchiy, N, Almeida, GM, Jones, GDD, Johnson, GE, Parry, JM, Rothkamm, K & Dubrova, YE 2006, 'Radiation-induced transgenerational alterations in genome stability and DNA damage', ONCOGENE, Jg. 25, Nr. 56, S. 7336-42. https://doi.org/10.1038/sj.onc.1209723

APA

Barber, R. C., Hickenbotham, P., Hatch, T., Kelly, D., Topchiy, N., Almeida, G. M., Jones, G. D. D., Johnson, G. E., Parry, J. M., Rothkamm, K., & Dubrova, Y. E. (2006). Radiation-induced transgenerational alterations in genome stability and DNA damage. ONCOGENE, 25(56), 7336-42. https://doi.org/10.1038/sj.onc.1209723

Vancouver

Barber RC, Hickenbotham P, Hatch T, Kelly D, Topchiy N, Almeida GM et al. Radiation-induced transgenerational alterations in genome stability and DNA damage. ONCOGENE. 2006 Nov 30;25(56):7336-42. https://doi.org/10.1038/sj.onc.1209723

Bibtex

@article{9fea6fb319fa4276a7787ac343baa7a4,
title = "Radiation-induced transgenerational alterations in genome stability and DNA damage",
abstract = "Mutation induction in directly exposed cells is currently regarded as the main component of the genetic risk of ionizing radiation for humans. However, recent data on the transgenerational increases in mutation rates in the offspring of irradiated parents indicate that the genetic risk could be greater than predicted previously. Here, we have analysed transgenerational changes in mutation rates and DNA damage in the germline and somatic tissues of non-exposed first-generation offspring of irradiated inbred male CBA/Ca and BALB/c mice. Mutation rates at an expanded simple tandem repeat DNA locus and a protein-coding gene (hprt) were significantly elevated in both the germline (sperm) and somatic tissues of all the offspring of irradiated males. The transgenerational changes in mutation rates were attributed to the presence of a persistent subset of endogenous DNA lesions (double- and single-strand breaks), measured by the phosphorylated form of histone H2AX (gamma-H2AX) and alkaline Comet assays. Such remarkable transgenerational destabilization of the F(1) genome may have important implications for cancer aetiology and genetic risk estimates. Our data also provide important clues on the still unknown mechanisms of radiation-induced genomic instability.",
keywords = "Animals, Base Sequence, Comet Assay, DNA/radiation effects, DNA Damage, DNA Primers, DNA Repair, Genomic Instability, Hypoxanthine Phosphoribosyltransferase/genetics, Male, Mice, Mice, Inbred BALB C, Mice, Inbred CBA, Mutation, Tandem Repeat Sequences",
author = "Barber, {R C} and P Hickenbotham and T Hatch and D Kelly and N Topchiy and Almeida, {G M} and Jones, {G D D} and Johnson, {G E} and Parry, {J M} and K Rothkamm and Dubrova, {Y E}",
year = "2006",
month = nov,
day = "30",
doi = "10.1038/sj.onc.1209723",
language = "English",
volume = "25",
pages = "7336--42",
journal = "ONCOGENE",
issn = "0950-9232",
publisher = "NATURE PUBLISHING GROUP",
number = "56",

}

RIS

TY - JOUR

T1 - Radiation-induced transgenerational alterations in genome stability and DNA damage

AU - Barber, R C

AU - Hickenbotham, P

AU - Hatch, T

AU - Kelly, D

AU - Topchiy, N

AU - Almeida, G M

AU - Jones, G D D

AU - Johnson, G E

AU - Parry, J M

AU - Rothkamm, K

AU - Dubrova, Y E

PY - 2006/11/30

Y1 - 2006/11/30

N2 - Mutation induction in directly exposed cells is currently regarded as the main component of the genetic risk of ionizing radiation for humans. However, recent data on the transgenerational increases in mutation rates in the offspring of irradiated parents indicate that the genetic risk could be greater than predicted previously. Here, we have analysed transgenerational changes in mutation rates and DNA damage in the germline and somatic tissues of non-exposed first-generation offspring of irradiated inbred male CBA/Ca and BALB/c mice. Mutation rates at an expanded simple tandem repeat DNA locus and a protein-coding gene (hprt) were significantly elevated in both the germline (sperm) and somatic tissues of all the offspring of irradiated males. The transgenerational changes in mutation rates were attributed to the presence of a persistent subset of endogenous DNA lesions (double- and single-strand breaks), measured by the phosphorylated form of histone H2AX (gamma-H2AX) and alkaline Comet assays. Such remarkable transgenerational destabilization of the F(1) genome may have important implications for cancer aetiology and genetic risk estimates. Our data also provide important clues on the still unknown mechanisms of radiation-induced genomic instability.

AB - Mutation induction in directly exposed cells is currently regarded as the main component of the genetic risk of ionizing radiation for humans. However, recent data on the transgenerational increases in mutation rates in the offspring of irradiated parents indicate that the genetic risk could be greater than predicted previously. Here, we have analysed transgenerational changes in mutation rates and DNA damage in the germline and somatic tissues of non-exposed first-generation offspring of irradiated inbred male CBA/Ca and BALB/c mice. Mutation rates at an expanded simple tandem repeat DNA locus and a protein-coding gene (hprt) were significantly elevated in both the germline (sperm) and somatic tissues of all the offspring of irradiated males. The transgenerational changes in mutation rates were attributed to the presence of a persistent subset of endogenous DNA lesions (double- and single-strand breaks), measured by the phosphorylated form of histone H2AX (gamma-H2AX) and alkaline Comet assays. Such remarkable transgenerational destabilization of the F(1) genome may have important implications for cancer aetiology and genetic risk estimates. Our data also provide important clues on the still unknown mechanisms of radiation-induced genomic instability.

KW - Animals

KW - Base Sequence

KW - Comet Assay

KW - DNA/radiation effects

KW - DNA Damage

KW - DNA Primers

KW - DNA Repair

KW - Genomic Instability

KW - Hypoxanthine Phosphoribosyltransferase/genetics

KW - Male

KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, Inbred CBA

KW - Mutation

KW - Tandem Repeat Sequences

U2 - 10.1038/sj.onc.1209723

DO - 10.1038/sj.onc.1209723

M3 - SCORING: Journal article

C2 - 16751800

VL - 25

SP - 7336

EP - 7342

JO - ONCOGENE

JF - ONCOGENE

SN - 0950-9232

IS - 56

ER -