Quantitative high-resolution genomic analysis of single cancer cells.
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Quantitative high-resolution genomic analysis of single cancer cells. / Hannemann, Juliane; Meyer-Staeckling, Sönke; Kemming, Dirk; Alpers, Iris; Joosse, Simon; Pospisil, Heike; Kurtz, Stefan; Görndt, Jennifer; Püschel, Klaus; Riethdorf, Sabine; Pantel, Klaus; Brandt, Burkhard.
in: PLOS ONE, Jahrgang 6, Nr. 11, 11, 2011, S. 26362.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Quantitative high-resolution genomic analysis of single cancer cells.
AU - Hannemann, Juliane
AU - Meyer-Staeckling, Sönke
AU - Kemming, Dirk
AU - Alpers, Iris
AU - Joosse, Simon
AU - Pospisil, Heike
AU - Kurtz, Stefan
AU - Görndt, Jennifer
AU - Püschel, Klaus
AU - Riethdorf, Sabine
AU - Pantel, Klaus
AU - Brandt, Burkhard
PY - 2011
Y1 - 2011
N2 - During cancer progression, specific genomic aberrations arise that can determine the scope of the disease and can be used as predictive or prognostic markers. The detection of specific gene amplifications or deletions in single blood-borne or disseminated tumour cells that may give rise to the development of metastases is of great clinical interest but technically challenging. In this study, we present a method for quantitative high-resolution genomic analysis of single cells. Cells were isolated under permanent microscopic control followed by high-fidelity whole genome amplification and subsequent analyses by fine tiling array-CGH and qPCR. The assay was applied to single breast cancer cells to analyze the chromosomal region centred by the therapeutical relevant EGFR gene. This method allows precise quantitative analysis of copy number variations in single cell diagnostics.
AB - During cancer progression, specific genomic aberrations arise that can determine the scope of the disease and can be used as predictive or prognostic markers. The detection of specific gene amplifications or deletions in single blood-borne or disseminated tumour cells that may give rise to the development of metastases is of great clinical interest but technically challenging. In this study, we present a method for quantitative high-resolution genomic analysis of single cells. Cells were isolated under permanent microscopic control followed by high-fidelity whole genome amplification and subsequent analyses by fine tiling array-CGH and qPCR. The assay was applied to single breast cancer cells to analyze the chromosomal region centred by the therapeutical relevant EGFR gene. This method allows precise quantitative analysis of copy number variations in single cell diagnostics.
KW - Humans
KW - Cell Line, Tumor
KW - Comparative Genomic Hybridization
KW - Polymerase Chain Reaction
KW - Genetic Heterogeneity
KW - Genomics/methods
KW - Neoplasms/blood/genetics/pathology
KW - Receptor, Epidermal Growth Factor/genetics
KW - Single-Cell Analysis/methods
KW - Humans
KW - Cell Line, Tumor
KW - Comparative Genomic Hybridization
KW - Polymerase Chain Reaction
KW - Genetic Heterogeneity
KW - Genomics/methods
KW - Neoplasms/blood/genetics/pathology
KW - Receptor, Epidermal Growth Factor/genetics
KW - Single-Cell Analysis/methods
U2 - 10.1371/journal.pone.0026362
DO - 10.1371/journal.pone.0026362
M3 - SCORING: Journal article
VL - 6
SP - 26362
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 11
M1 - 11
ER -
