Purification, crystallization and preliminary X-ray diffraction analysis of ThiM from Staphylococcus aureus.
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Purification, crystallization and preliminary X-ray diffraction analysis of ThiM from Staphylococcus aureus. / Drebes, Julia; Perbandt, Markus; Wrenger, Carsten; Betzel, Christian.
in: ACTA CRYSTALLOGR F, Jahrgang 67, Nr. Pt 4, Pt 4, 2011, S. 479-481.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Purification, crystallization and preliminary X-ray diffraction analysis of ThiM from Staphylococcus aureus.
AU - Drebes, Julia
AU - Perbandt, Markus
AU - Wrenger, Carsten
AU - Betzel, Christian
PY - 2011
Y1 - 2011
N2 - ThiM [5-(hydroxyethyl)-4-methylthiazole kinase; EC 2.7.1.50] from Staphylococcus aureus is an essential enzyme of thiamine or vitamin B(1) metabolism and has been crystallized by the vapour-diffusion method. The crystals belonged to the primitive space group P1, with unit-cell parameters a = 62.06, b = 62.40, c = 107.82?Å, ? = 92.25, ? = 91.37, ? = 101.48° and six protomers in the unit cell, corresponding to a packing parameter V(M) of 2.3?Å(3)?Da(-1). Diffraction data were collected to 2.1?Å resolution using synchrotron radiation. The phase problem was solved by molecular replacement.
AB - ThiM [5-(hydroxyethyl)-4-methylthiazole kinase; EC 2.7.1.50] from Staphylococcus aureus is an essential enzyme of thiamine or vitamin B(1) metabolism and has been crystallized by the vapour-diffusion method. The crystals belonged to the primitive space group P1, with unit-cell parameters a = 62.06, b = 62.40, c = 107.82?Å, ? = 92.25, ? = 91.37, ? = 101.48° and six protomers in the unit cell, corresponding to a packing parameter V(M) of 2.3?Å(3)?Da(-1). Diffraction data were collected to 2.1?Å resolution using synchrotron radiation. The phase problem was solved by molecular replacement.
KW - Crystallography, X-Ray
KW - Crystallization
KW - Phosphotransferases (Alcohol Group Acceptor)/chemistry/isolation & purification
KW - Staphylococcus aureus/enzymology
KW - Crystallography, X-Ray
KW - Crystallization
KW - Phosphotransferases (Alcohol Group Acceptor)/chemistry/isolation & purification
KW - Staphylococcus aureus/enzymology
M3 - SCORING: Journal article
VL - 67
SP - 479
EP - 481
JO - ACTA CRYSTALLOGR F
JF - ACTA CRYSTALLOGR F
SN - 2053-230X
IS - Pt 4
M1 - Pt 4
ER -