Protocol for differential multi-omic analyses of distinct cell types in the mouse cerebral cortex

Standard

Protocol for differential multi-omic analyses of distinct cell types in the mouse cerebral cortex. / Meka, Durga Praveen; Richter, Melanie; Rücker, Tabitha; Voss, Hannah; Rissiek, Anne; Krisp, Christoph; Kumar, Nisha Hemandhar; Schwanke, Birgit; Fornasiero, Eugenio F; Schlüter, Hartmut; Calderon de Anda, Froylan.

in: STAR PROTOC, Jahrgang 5, Nr. 1, 15.03.2024, S. 102793.

Publikationen: SCORING: Beitrag in Fachzeitschrift/ZeitungSCORING: ZeitschriftenaufsatzForschungBegutachtung

Harvard

Meka, DP, Richter, M, Rücker, T, Voss, H, Rissiek, A, Krisp, C, Kumar, NH, Schwanke, B, Fornasiero, EF, Schlüter, H & Calderon de Anda, F 2024, 'Protocol for differential multi-omic analyses of distinct cell types in the mouse cerebral cortex', STAR PROTOC, Jg. 5, Nr. 1, S. 102793. https://doi.org/10.1016/j.xpro.2023.102793

APA

Meka, D. P., Richter, M., Rücker, T., Voss, H., Rissiek, A., Krisp, C., Kumar, N. H., Schwanke, B., Fornasiero, E. F., Schlüter, H., & Calderon de Anda, F. (2024). Protocol for differential multi-omic analyses of distinct cell types in the mouse cerebral cortex. STAR PROTOC, 5(1), 102793. https://doi.org/10.1016/j.xpro.2023.102793

Vancouver

Bibtex

@article{2573f71fea42422ea4d9150fd1d19a92,
title = "Protocol for differential multi-omic analyses of distinct cell types in the mouse cerebral cortex",
abstract = "Here, we present a protocol for differential multi-omic analyses of distinct cell types in the developing mouse cerebral cortex. We describe steps for in utero electroporation, subsequent flow-cytometry-based isolation of developing mouse cortical cells, bulk RNA sequencing or quantitative liquid chromatography-tandem mass spectrometry, and bioinformatic analyses. This protocol can be applied to compare the proteomes and transcriptomes of developing mouse cortical cell populations after various manipulations (e.g., epigenetic). For complete details on the use and execution of this protocol, please refer to Meka et al. (2022).1.",
author = "Meka, {Durga Praveen} and Melanie Richter and Tabitha R{\"u}cker and Hannah Voss and Anne Rissiek and Christoph Krisp and Kumar, {Nisha Hemandhar} and Birgit Schwanke and Fornasiero, {Eugenio F} and Hartmut Schl{\"u}ter and {Calderon de Anda}, Froylan",
note = "Copyright {\textcopyright} 2023 The Authors. Published by Elsevier Inc. All rights reserved.",
year = "2024",
month = mar,
day = "15",
doi = "10.1016/j.xpro.2023.102793",
language = "English",
volume = "5",
pages = "102793",
journal = "STAR PROTOC",
issn = "2666-1667",
publisher = "Cell Press",
number = "1",

}

RIS

TY - JOUR

T1 - Protocol for differential multi-omic analyses of distinct cell types in the mouse cerebral cortex

AU - Meka, Durga Praveen

AU - Richter, Melanie

AU - Rücker, Tabitha

AU - Voss, Hannah

AU - Rissiek, Anne

AU - Krisp, Christoph

AU - Kumar, Nisha Hemandhar

AU - Schwanke, Birgit

AU - Fornasiero, Eugenio F

AU - Schlüter, Hartmut

AU - Calderon de Anda, Froylan

N1 - Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2024/3/15

Y1 - 2024/3/15

N2 - Here, we present a protocol for differential multi-omic analyses of distinct cell types in the developing mouse cerebral cortex. We describe steps for in utero electroporation, subsequent flow-cytometry-based isolation of developing mouse cortical cells, bulk RNA sequencing or quantitative liquid chromatography-tandem mass spectrometry, and bioinformatic analyses. This protocol can be applied to compare the proteomes and transcriptomes of developing mouse cortical cell populations after various manipulations (e.g., epigenetic). For complete details on the use and execution of this protocol, please refer to Meka et al. (2022).1.

AB - Here, we present a protocol for differential multi-omic analyses of distinct cell types in the developing mouse cerebral cortex. We describe steps for in utero electroporation, subsequent flow-cytometry-based isolation of developing mouse cortical cells, bulk RNA sequencing or quantitative liquid chromatography-tandem mass spectrometry, and bioinformatic analyses. This protocol can be applied to compare the proteomes and transcriptomes of developing mouse cortical cell populations after various manipulations (e.g., epigenetic). For complete details on the use and execution of this protocol, please refer to Meka et al. (2022).1.

U2 - 10.1016/j.xpro.2023.102793

DO - 10.1016/j.xpro.2023.102793

M3 - SCORING: Journal article

C2 - 38157295

VL - 5

SP - 102793

JO - STAR PROTOC

JF - STAR PROTOC

SN - 2666-1667

IS - 1

ER -