Proteomic patterns predict acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation.
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Proteomic patterns predict acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation. / Weissinger, Eva M; Schiffer, Eric; Hertenstein, Bernd; Ferrara, James L; Holler, Ernst; Stadler, Michael; Kolb, Hans-Jochem; Zander, Axel R.; Zürbig, Petra; Kellmann, Markus; Ganser, Arnold.
in: BLOOD, Jahrgang 109, Nr. 12, 12, 2007, S. 5511-5519.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Proteomic patterns predict acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation.
AU - Weissinger, Eva M
AU - Schiffer, Eric
AU - Hertenstein, Bernd
AU - Ferrara, James L
AU - Holler, Ernst
AU - Stadler, Michael
AU - Kolb, Hans-Jochem
AU - Zander, Axel R.
AU - Zürbig, Petra
AU - Kellmann, Markus
AU - Ganser, Arnold
PY - 2007
Y1 - 2007
N2 - Acute graft-versus-host disease (aGvHD) contributes significantly to morbidity and mortality after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Diagnosis of GvHD is mainly based on clinical features and tissue biopsies. A noninvasive, unbiased laboratory test for GvHD diagnosis does not exist. Here we describe the application of capillary electrophoresis coupled online with mass spectrometry (CE-MS) to 13 samples from 10 patients with aGvHD of grade II or more and 50 control samples from 23 patients without GvHD. About 170 GvHD-specific polypeptides were detected and a tentatively aGvHD-specific model consisting of 31 polypeptides was chosen, allowing correct classification of 13 of 13 (sensitivity 100.0% [95% confidence interval {CI} 75.1 to 100.0]) aGvHD samples and 49 of 50 (specificity 98.0% [95% CI 89.3 to 99.7]) control samples of the training set. The subsequent blinded evaluation of 599 samples enabled diagnosis of aGvHD greater than grade II, even prior to clinical diagnosis, with a sensitivity of 83.1% (95% CI 73.1 to 87.9) and a specificity of 75.6% (95% CI 71.6 to 79.4). Thus, high-resolution proteome analysis represents an unbiased laboratory-based screening method, enabling diagnosis, and possibly enabling preemptive therapy.
AB - Acute graft-versus-host disease (aGvHD) contributes significantly to morbidity and mortality after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Diagnosis of GvHD is mainly based on clinical features and tissue biopsies. A noninvasive, unbiased laboratory test for GvHD diagnosis does not exist. Here we describe the application of capillary electrophoresis coupled online with mass spectrometry (CE-MS) to 13 samples from 10 patients with aGvHD of grade II or more and 50 control samples from 23 patients without GvHD. About 170 GvHD-specific polypeptides were detected and a tentatively aGvHD-specific model consisting of 31 polypeptides was chosen, allowing correct classification of 13 of 13 (sensitivity 100.0% [95% confidence interval {CI} 75.1 to 100.0]) aGvHD samples and 49 of 50 (specificity 98.0% [95% CI 89.3 to 99.7]) control samples of the training set. The subsequent blinded evaluation of 599 samples enabled diagnosis of aGvHD greater than grade II, even prior to clinical diagnosis, with a sensitivity of 83.1% (95% CI 73.1 to 87.9) and a specificity of 75.6% (95% CI 71.6 to 79.4). Thus, high-resolution proteome analysis represents an unbiased laboratory-based screening method, enabling diagnosis, and possibly enabling preemptive therapy.
M3 - SCORING: Zeitschriftenaufsatz
VL - 109
SP - 5511
EP - 5519
JO - BLOOD
JF - BLOOD
SN - 0006-4971
IS - 12
M1 - 12
ER -
