Proteomic Comparison of Malignant Human Germ Cell Tumor Cell Lines
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Proteomic Comparison of Malignant Human Germ Cell Tumor Cell Lines. / Bremmer, Felix; Bohnenberger, Hanibal; Küffer, Stefan; Oellerich, Thomas; Serve, Hubert; Urlaub, Henning; Strauss, Arne; Maatoug, Yasmine; Behnes, Carl Ludwig; Oing, Christoph; Radzun, Heinz Joachim; Ströbel, Philipp; Balabanov, Stefan; Honecker, Friedemann.
in: DIS MARKERS, Jahrgang 2019, 2019, S. 8298524.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Proteomic Comparison of Malignant Human Germ Cell Tumor Cell Lines
AU - Bremmer, Felix
AU - Bohnenberger, Hanibal
AU - Küffer, Stefan
AU - Oellerich, Thomas
AU - Serve, Hubert
AU - Urlaub, Henning
AU - Strauss, Arne
AU - Maatoug, Yasmine
AU - Behnes, Carl Ludwig
AU - Oing, Christoph
AU - Radzun, Heinz Joachim
AU - Ströbel, Philipp
AU - Balabanov, Stefan
AU - Honecker, Friedemann
N1 - Copyright © 2019 Felix Bremmer et al.
PY - 2019
Y1 - 2019
N2 - Malignant germ cell tumors (GCT) are the most common malignant tumors in young men between 18 and 40 years. The correct identification of histological subtypes, in difficult cases supported by immunohistochemistry, is essential for therapeutic management. Furthermore, biomarkers may help to understand pathophysiological processes in these tumor types. Two GCT cell lines, TCam-2 with seminoma-like characteristics, and NTERA-2, an embryonal carcinoma-like cell line, were compared by a quantitative proteomic approach using high-resolution mass spectrometry (MS) in combination with stable isotope labelling by amino acid in cell culture (SILAC). We were able to identify 4856 proteins and quantify the expression of 3936. 347 were significantly differentially expressed between the two cell lines. For further validation, CD81, CBX-3, PHF6, and ENSA were analyzed by western blot analysis. The results confirmed the MS results. Immunohistochemical analysis on 59 formalin-fixed and paraffin-embedded (FFPE) normal and GCT tissue samples (normal testis, GCNIS, seminomas, and embryonal carcinomas) of these proteins demonstrated the ability to distinguish different GCT subtypes, especially seminomas and embryonal carcinomas. In addition, siRNA-mediated knockdown of these proteins resulted in an antiproliferative effect in TCam-2, NTERA-2, and an additional embryonal carcinoma-like cell line, NCCIT. In summary, this study represents a proteomic resource for the discrimination of malignant germ cell tumor subtypes and the observed antiproliferative effect after knockdown of selected proteins paves the way for the identification of new potential drug targets.
AB - Malignant germ cell tumors (GCT) are the most common malignant tumors in young men between 18 and 40 years. The correct identification of histological subtypes, in difficult cases supported by immunohistochemistry, is essential for therapeutic management. Furthermore, biomarkers may help to understand pathophysiological processes in these tumor types. Two GCT cell lines, TCam-2 with seminoma-like characteristics, and NTERA-2, an embryonal carcinoma-like cell line, were compared by a quantitative proteomic approach using high-resolution mass spectrometry (MS) in combination with stable isotope labelling by amino acid in cell culture (SILAC). We were able to identify 4856 proteins and quantify the expression of 3936. 347 were significantly differentially expressed between the two cell lines. For further validation, CD81, CBX-3, PHF6, and ENSA were analyzed by western blot analysis. The results confirmed the MS results. Immunohistochemical analysis on 59 formalin-fixed and paraffin-embedded (FFPE) normal and GCT tissue samples (normal testis, GCNIS, seminomas, and embryonal carcinomas) of these proteins demonstrated the ability to distinguish different GCT subtypes, especially seminomas and embryonal carcinomas. In addition, siRNA-mediated knockdown of these proteins resulted in an antiproliferative effect in TCam-2, NTERA-2, and an additional embryonal carcinoma-like cell line, NCCIT. In summary, this study represents a proteomic resource for the discrimination of malignant germ cell tumor subtypes and the observed antiproliferative effect after knockdown of selected proteins paves the way for the identification of new potential drug targets.
U2 - 10.1155/2019/8298524
DO - 10.1155/2019/8298524
M3 - SCORING: Journal article
C2 - 31565104
VL - 2019
SP - 8298524
JO - DIS MARKERS
JF - DIS MARKERS
SN - 0278-0240
ER -
