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Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress

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Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress. / Wright, Bradley W; Kamath, Karthik S; Krisp, Christoph; Molloy, Mark P.

in: BMC MICROBIOL, Jahrgang 19, Nr. 1, 01.04.2019, S. 69.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Wright, BW, Kamath, KS, Krisp, C & Molloy, MP 2019, 'Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress', BMC MICROBIOL, Jg. 19, Nr. 1, S. 69. https://doi.org/10.1186/s12866-019-1441-7

APA

Wright, B. W., Kamath, K. S., Krisp, C., & Molloy, M. P. (2019). Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress. BMC MICROBIOL, 19(1), 69. https://doi.org/10.1186/s12866-019-1441-7

Vancouver

Wright BW, Kamath KS, Krisp C, Molloy MP. Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress. BMC MICROBIOL. 2019 Apr 1;19(1):69. https://doi.org/10.1186/s12866-019-1441-7

Bibtex

@article{99230862b4b3443195e89ed997ebdf4c,
title = "Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress",
abstract = "BACKGROUND: The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medically relevant copper-stress condition.RESULTS: We used data-independent acquisition mass spectrometry to quantitate protein expression changes associated with copper stress in P. aeruginosa PAO1. Approximately 2000 non-redundant proteins were quantified, with 78 proteins altering in abundance by +/- 1.5-fold or more when cultured to mid-log growth in the presence of 50 μM copper sulfate. One-third of those differentially expressed proteins have no prior established functional roles.CONCLUSIONS: This study provides evidence for the functional involvement of some specific proteins in enabling P. aeruginosa to survive under sub-lethal concentrations of copper. This further paves the way for targeted investigations into the specific mechanisms of their activity.",
author = "Wright, {Bradley W} and Kamath, {Karthik S} and Christoph Krisp and Molloy, {Mark P}",
year = "2019",
month = apr,
day = "1",
doi = "10.1186/s12866-019-1441-7",
language = "English",
volume = "19",
pages = "69",
journal = "BMC MICROBIOL",
issn = "1471-2180",
publisher = "BioMed Central Ltd.",
number = "1",

}

RIS

TY - JOUR

T1 - Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress

AU - Wright, Bradley W

AU - Kamath, Karthik S

AU - Krisp, Christoph

AU - Molloy, Mark P

PY - 2019/4/1

Y1 - 2019/4/1

N2 - BACKGROUND: The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medically relevant copper-stress condition.RESULTS: We used data-independent acquisition mass spectrometry to quantitate protein expression changes associated with copper stress in P. aeruginosa PAO1. Approximately 2000 non-redundant proteins were quantified, with 78 proteins altering in abundance by +/- 1.5-fold or more when cultured to mid-log growth in the presence of 50 μM copper sulfate. One-third of those differentially expressed proteins have no prior established functional roles.CONCLUSIONS: This study provides evidence for the functional involvement of some specific proteins in enabling P. aeruginosa to survive under sub-lethal concentrations of copper. This further paves the way for targeted investigations into the specific mechanisms of their activity.

AB - BACKGROUND: The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medically relevant copper-stress condition.RESULTS: We used data-independent acquisition mass spectrometry to quantitate protein expression changes associated with copper stress in P. aeruginosa PAO1. Approximately 2000 non-redundant proteins were quantified, with 78 proteins altering in abundance by +/- 1.5-fold or more when cultured to mid-log growth in the presence of 50 μM copper sulfate. One-third of those differentially expressed proteins have no prior established functional roles.CONCLUSIONS: This study provides evidence for the functional involvement of some specific proteins in enabling P. aeruginosa to survive under sub-lethal concentrations of copper. This further paves the way for targeted investigations into the specific mechanisms of their activity.

U2 - 10.1186/s12866-019-1441-7

DO - 10.1186/s12866-019-1441-7

M3 - SCORING: Journal article

C2 - 30935370

VL - 19

SP - 69

JO - BMC MICROBIOL

JF - BMC MICROBIOL

SN - 1471-2180

IS - 1

ER -