Proliferation of fetal rat hepatocytes in response to growth factors and hormones in primary culture.
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Proliferation of fetal rat hepatocytes in response to growth factors and hormones in primary culture. / Hoffmann, B; Piasecki, Angelika; Paul, D.
in: J CELL PHYSIOL, Jahrgang 139, Nr. 3, 3, 1989, S. 654-662.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Proliferation of fetal rat hepatocytes in response to growth factors and hormones in primary culture.
AU - Hoffmann, B
AU - Piasecki, Angelika
AU - Paul, D
PY - 1989
Y1 - 1989
N2 - Fetal rat hepatocytes (day 19 of gestation) multiply in primary culture in arginine-free, hydrocortisone-containing chemically defined medium MX-82 supplemented either with epidermal growth factor (EGF) or insulin or both. In contrast, hepatocytes did not multiply under similar culture conditions using Dulbecco's minimum essential medium (DMEM). Cells underwent two divisions within 10 days in cultures maintained in MX-82 medium without a medium change, and cells grew to increased final cell densities when the medium was renewed every third day. When the medium MX-82 was enriched by the addition of lipids, intermediary metabolites, and trace metals (medium MX-83), cells grew to higher densities. In the absence of the growth factors, cells became quiescent and subsequently could be induced to synthesize DNA in response to EGF. With the increasing numbers of cells per dish, the growth response of the hepatocytes diminished. Levels of hepatocyte-specific albumin and alpha-fetoprotein mRNAs at day 0 were similar to those observed at day 10 in primary fetal rat hepatocyte cultures and were maintained at higher levels in medium MX-83 than in medium MX-82.
AB - Fetal rat hepatocytes (day 19 of gestation) multiply in primary culture in arginine-free, hydrocortisone-containing chemically defined medium MX-82 supplemented either with epidermal growth factor (EGF) or insulin or both. In contrast, hepatocytes did not multiply under similar culture conditions using Dulbecco's minimum essential medium (DMEM). Cells underwent two divisions within 10 days in cultures maintained in MX-82 medium without a medium change, and cells grew to increased final cell densities when the medium was renewed every third day. When the medium MX-82 was enriched by the addition of lipids, intermediary metabolites, and trace metals (medium MX-83), cells grew to higher densities. In the absence of the growth factors, cells became quiescent and subsequently could be induced to synthesize DNA in response to EGF. With the increasing numbers of cells per dish, the growth response of the hepatocytes diminished. Levels of hepatocyte-specific albumin and alpha-fetoprotein mRNAs at day 0 were similar to those observed at day 10 in primary fetal rat hepatocyte cultures and were maintained at higher levels in medium MX-83 than in medium MX-82.
KW - Animals
KW - Cells, Cultured
KW - Rats
KW - Cell Division
KW - Cell Separation
KW - Fetus
KW - Culture Media
KW - DNA Replication
KW - Liver/cytology/drug effects
KW - Culture Techniques/methods
KW - DNA/biosynthesis
KW - Epidermal Growth Factor/pharmacology
KW - Insulin/pharmacology
KW - RNA/genetics/isolation & purification
KW - Rats, Inbred Strains
KW - Serum Albumin/biosynthesis
KW - alpha-Fetoproteins/biosynthesis
KW - Animals
KW - Cells, Cultured
KW - Rats
KW - Cell Division
KW - Cell Separation
KW - Fetus
KW - Culture Media
KW - DNA Replication
KW - Liver/cytology/drug effects
KW - Culture Techniques/methods
KW - DNA/biosynthesis
KW - Epidermal Growth Factor/pharmacology
KW - Insulin/pharmacology
KW - RNA/genetics/isolation & purification
KW - Rats, Inbred Strains
KW - Serum Albumin/biosynthesis
KW - alpha-Fetoproteins/biosynthesis
M3 - SCORING: Journal article
VL - 139
SP - 654
EP - 662
JO - J CELL PHYSIOL
JF - J CELL PHYSIOL
SN - 0021-9541
IS - 3
M1 - 3
ER -
