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Prolactin--a novel neuroendocrine regulator of human keratin expression in situ

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Prolactin--a novel neuroendocrine regulator of human keratin expression in situ. / Ramot, Yuval; Bíró, Tamás; Tiede, Stephan; Tóth, Balázs I; Langan, Ewan A; Sugawara, Koji; Foitzik, Kerstin; Ingber, Arieh; Goffin, Vincent; Langbein, Lutz; Paus, Ralf.

in: FASEB J, Jahrgang 24, Nr. 6, 06.2010, S. 1768-79.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Ramot, Y, Bíró, T, Tiede, S, Tóth, BI, Langan, EA, Sugawara, K, Foitzik, K, Ingber, A, Goffin, V, Langbein, L & Paus, R 2010, 'Prolactin--a novel neuroendocrine regulator of human keratin expression in situ', FASEB J, Jg. 24, Nr. 6, S. 1768-79. https://doi.org/10.1096/fj.09-146415

APA

Ramot, Y., Bíró, T., Tiede, S., Tóth, B. I., Langan, E. A., Sugawara, K., Foitzik, K., Ingber, A., Goffin, V., Langbein, L., & Paus, R. (2010). Prolactin--a novel neuroendocrine regulator of human keratin expression in situ. FASEB J, 24(6), 1768-79. https://doi.org/10.1096/fj.09-146415

Vancouver

Ramot Y, Bíró T, Tiede S, Tóth BI, Langan EA, Sugawara K et al. Prolactin--a novel neuroendocrine regulator of human keratin expression in situ. FASEB J. 2010 Jun;24(6):1768-79. https://doi.org/10.1096/fj.09-146415

Bibtex

@article{3c97b67892964f1ab6ad1c5fb71ab843,
title = "Prolactin--a novel neuroendocrine regulator of human keratin expression in situ",
abstract = "The controls of human keratin expression in situ remain to be fully elucidated. Here, we have investigated the effects of the neurohormone prolactin (PRL) on keratin expression in a physiologically and clinically relevant test system: organ-cultured normal human hair follicles (HFs). Not only do HFs express a wide range of keratins, but they are also a source and target of PRL. Microarray analysis revealed that PRL differentially regulated a defined subset of keratins and keratin-associated proteins. Quantitative immunohistomorphometry and quantitative PCR confirmed that PRL up-regulated expression of keratins K5 and K14 and the epithelial stem cell-associated keratins K15 and K19 in organ-cultured HFs and/or isolated HF keratinocytes. PRL also up-regulated K15 promoter activity and K15 protein expression in situ, whereas it inhibited K6 and K31 expression. These regulatory effects were reversed by a pure competitive PRL receptor antagonist. Antagonist alone also modulated keratin expression, suggesting that {"}tonic stimulation{"} by endogenous PRL is required for normal expression levels of selected keratins. Therefore, our study identifies PRL as a major, clinically relevant, novel neuroendocrine regulator of both human keratin expression and human epithelial stem cell biology in situ.",
keywords = "Adult, Aged, Biomarkers, Blotting, Western, Female, Gene Expression Profiling, Hair Follicle, Humans, Immunoenzyme Techniques, Keratinocytes, Keratins, Middle Aged, Oligonucleotide Array Sequence Analysis, Organ Culture Techniques, Prolactin, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Journal Article, Research Support, Non-U.S. Gov't",
author = "Yuval Ramot and Tam{\'a}s B{\'i}r{\'o} and Stephan Tiede and T{\'o}th, {Bal{\'a}zs I} and Langan, {Ewan A} and Koji Sugawara and Kerstin Foitzik and Arieh Ingber and Vincent Goffin and Lutz Langbein and Ralf Paus",
year = "2010",
month = jun,
doi = "10.1096/fj.09-146415",
language = "English",
volume = "24",
pages = "1768--79",
journal = "FASEB J",
issn = "0892-6638",
publisher = "FASEB",
number = "6",

}

RIS

TY - JOUR

T1 - Prolactin--a novel neuroendocrine regulator of human keratin expression in situ

AU - Ramot, Yuval

AU - Bíró, Tamás

AU - Tiede, Stephan

AU - Tóth, Balázs I

AU - Langan, Ewan A

AU - Sugawara, Koji

AU - Foitzik, Kerstin

AU - Ingber, Arieh

AU - Goffin, Vincent

AU - Langbein, Lutz

AU - Paus, Ralf

PY - 2010/6

Y1 - 2010/6

N2 - The controls of human keratin expression in situ remain to be fully elucidated. Here, we have investigated the effects of the neurohormone prolactin (PRL) on keratin expression in a physiologically and clinically relevant test system: organ-cultured normal human hair follicles (HFs). Not only do HFs express a wide range of keratins, but they are also a source and target of PRL. Microarray analysis revealed that PRL differentially regulated a defined subset of keratins and keratin-associated proteins. Quantitative immunohistomorphometry and quantitative PCR confirmed that PRL up-regulated expression of keratins K5 and K14 and the epithelial stem cell-associated keratins K15 and K19 in organ-cultured HFs and/or isolated HF keratinocytes. PRL also up-regulated K15 promoter activity and K15 protein expression in situ, whereas it inhibited K6 and K31 expression. These regulatory effects were reversed by a pure competitive PRL receptor antagonist. Antagonist alone also modulated keratin expression, suggesting that "tonic stimulation" by endogenous PRL is required for normal expression levels of selected keratins. Therefore, our study identifies PRL as a major, clinically relevant, novel neuroendocrine regulator of both human keratin expression and human epithelial stem cell biology in situ.

AB - The controls of human keratin expression in situ remain to be fully elucidated. Here, we have investigated the effects of the neurohormone prolactin (PRL) on keratin expression in a physiologically and clinically relevant test system: organ-cultured normal human hair follicles (HFs). Not only do HFs express a wide range of keratins, but they are also a source and target of PRL. Microarray analysis revealed that PRL differentially regulated a defined subset of keratins and keratin-associated proteins. Quantitative immunohistomorphometry and quantitative PCR confirmed that PRL up-regulated expression of keratins K5 and K14 and the epithelial stem cell-associated keratins K15 and K19 in organ-cultured HFs and/or isolated HF keratinocytes. PRL also up-regulated K15 promoter activity and K15 protein expression in situ, whereas it inhibited K6 and K31 expression. These regulatory effects were reversed by a pure competitive PRL receptor antagonist. Antagonist alone also modulated keratin expression, suggesting that "tonic stimulation" by endogenous PRL is required for normal expression levels of selected keratins. Therefore, our study identifies PRL as a major, clinically relevant, novel neuroendocrine regulator of both human keratin expression and human epithelial stem cell biology in situ.

KW - Adult

KW - Aged

KW - Biomarkers

KW - Blotting, Western

KW - Female

KW - Gene Expression Profiling

KW - Hair Follicle

KW - Humans

KW - Immunoenzyme Techniques

KW - Keratinocytes

KW - Keratins

KW - Middle Aged

KW - Oligonucleotide Array Sequence Analysis

KW - Organ Culture Techniques

KW - Prolactin

KW - RNA, Messenger

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1096/fj.09-146415

DO - 10.1096/fj.09-146415

M3 - SCORING: Journal article

C2 - 20103718

VL - 24

SP - 1768

EP - 1779

JO - FASEB J

JF - FASEB J

SN - 0892-6638

IS - 6

ER -