Progesterone-induced blocking factor (PIBF) and trophoblast invasiveness.
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Progesterone-induced blocking factor (PIBF) and trophoblast invasiveness. / Miko, E; Halasz, M; Jericevic-Mulac, B; Wicherek, L; Arck, Petra; Arató, G; Skret Magierlo, J; Rukavina, D; Szekeres-Bartho, J.
in: J REPROD IMMUNOL, Jahrgang 90, Nr. 1, 1, 2011, S. 50-57.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Progesterone-induced blocking factor (PIBF) and trophoblast invasiveness.
AU - Miko, E
AU - Halasz, M
AU - Jericevic-Mulac, B
AU - Wicherek, L
AU - Arck, Petra
AU - Arató, G
AU - Skret Magierlo, J
AU - Rukavina, D
AU - Szekeres-Bartho, J
PY - 2011
Y1 - 2011
N2 - Controlled trophoblast invasion is a key process during human placentation and a prerequisite for successful pregnancy. Progesterone is one of the factors to regulate trophoblast invasiveness. Progesterone-induced blocking factor (PIBF) is a progesterone-induced molecule expressed by the trophoblast, and also by tumors. The distribution of PIBF within the first-trimester decidua coincides with sites of trophoblast invasion. Another molecule that has been implicated in the control of trophoblast invasiveness is placental leptin. Leptin inhibits the secretion of progesterone by cytotrophoblast. The aim of this work was to investigate the possible interaction of PIBF and leptins in regulating trophoblast invasion. Paraffin-embedded sections from normal first-trimester placentae, partial moles, complete moles, and choriocarcinomas were reacted with PIBF, leptin, and leptin receptor specific antibodies. PIBF-deficient trophoblast cells were generated using siRNA and leptin receptor was detected on Western blot analysis. The lysates of PIBF-treated cells were used for detecting leptin expression in a protein array. PIBF was expressed in both normal first-trimester villous trophoblast and in partial mole. Compared with this, PIBF expression was markedly decreased in complete mole and absent in choriocarcinoma. Neither leptinR nor leptin were detected in partial mole, whereas both of these molecules were present in complete mole and choriocarcinoma. Leptin receptor expression was upregulated in PIBF-deficient cells, while leptin expression was decreased in PIBF-treated cells. These data suggest that PIBF affects the expression of leptin and its receptor, and that PIBF expression is inversely related to trophoblast invasiveness.
AB - Controlled trophoblast invasion is a key process during human placentation and a prerequisite for successful pregnancy. Progesterone is one of the factors to regulate trophoblast invasiveness. Progesterone-induced blocking factor (PIBF) is a progesterone-induced molecule expressed by the trophoblast, and also by tumors. The distribution of PIBF within the first-trimester decidua coincides with sites of trophoblast invasion. Another molecule that has been implicated in the control of trophoblast invasiveness is placental leptin. Leptin inhibits the secretion of progesterone by cytotrophoblast. The aim of this work was to investigate the possible interaction of PIBF and leptins in regulating trophoblast invasion. Paraffin-embedded sections from normal first-trimester placentae, partial moles, complete moles, and choriocarcinomas were reacted with PIBF, leptin, and leptin receptor specific antibodies. PIBF-deficient trophoblast cells were generated using siRNA and leptin receptor was detected on Western blot analysis. The lysates of PIBF-treated cells were used for detecting leptin expression in a protein array. PIBF was expressed in both normal first-trimester villous trophoblast and in partial mole. Compared with this, PIBF expression was markedly decreased in complete mole and absent in choriocarcinoma. Neither leptinR nor leptin were detected in partial mole, whereas both of these molecules were present in complete mole and choriocarcinoma. Leptin receptor expression was upregulated in PIBF-deficient cells, while leptin expression was decreased in PIBF-treated cells. These data suggest that PIBF affects the expression of leptin and its receptor, and that PIBF expression is inversely related to trophoblast invasiveness.
KW - Humans
KW - Female
KW - Blotting, Western
KW - Cell Line
KW - Pregnancy
KW - RNA Interference
KW - Choriocarcinoma/metabolism/pathology
KW - Decidua/metabolism/pathology
KW - Embryo Implantation/physiology
KW - Hydatidiform Mole/metabolism/pathology
KW - Leptin/biosynthesis/metabolism
KW - Placenta/metabolism/pathology
KW - Placentation/physiology
KW - Pregnancy Proteins/genetics/metabolism
KW - Pregnancy Trimester, First
KW - Progesterone/metabolism
KW - RNA, Small Interfering
KW - Receptors, Leptin/biosynthesis/immunology
KW - Suppressor Factors, Immunologic/genetics/metabolism
KW - Trophoblasts/metabolism
KW - Uterine Neoplasms/metabolism/pathology
KW - Humans
KW - Female
KW - Blotting, Western
KW - Cell Line
KW - Pregnancy
KW - RNA Interference
KW - Choriocarcinoma/metabolism/pathology
KW - Decidua/metabolism/pathology
KW - Embryo Implantation/physiology
KW - Hydatidiform Mole/metabolism/pathology
KW - Leptin/biosynthesis/metabolism
KW - Placenta/metabolism/pathology
KW - Placentation/physiology
KW - Pregnancy Proteins/genetics/metabolism
KW - Pregnancy Trimester, First
KW - Progesterone/metabolism
KW - RNA, Small Interfering
KW - Receptors, Leptin/biosynthesis/immunology
KW - Suppressor Factors, Immunologic/genetics/metabolism
KW - Trophoblasts/metabolism
KW - Uterine Neoplasms/metabolism/pathology
U2 - 10.1016/j.jri.2011.03.005
DO - 10.1016/j.jri.2011.03.005
M3 - SCORING: Journal article
VL - 90
SP - 50
EP - 57
JO - J REPROD IMMUNOL
JF - J REPROD IMMUNOL
SN - 0165-0378
IS - 1
M1 - 1
ER -
