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Profile of gene expression induced by the tumour promotor TPA in murine epithelial cells

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Profile of gene expression induced by the tumour promotor TPA in murine epithelial cells. / Schlingemann, Joerg; Hess, Jochen; Wrobel, Gunnar; Breitenbach, Ute; Gebhardt, Christoffer; Steinlein, Peter; Kramer, Heidi; Fürstenberger, Gerhard; Hahn, Meinhard; Angel, Peter; Lichter, Peter.

in: INT J CANCER, Jahrgang 104, Nr. 6, 10.05.2003, S. 699-708.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Schlingemann, J, Hess, J, Wrobel, G, Breitenbach, U, Gebhardt, C, Steinlein, P, Kramer, H, Fürstenberger, G, Hahn, M, Angel, P & Lichter, P 2003, 'Profile of gene expression induced by the tumour promotor TPA in murine epithelial cells', INT J CANCER, Jg. 104, Nr. 6, S. 699-708. https://doi.org/10.1002/ijc.11008

APA

Schlingemann, J., Hess, J., Wrobel, G., Breitenbach, U., Gebhardt, C., Steinlein, P., Kramer, H., Fürstenberger, G., Hahn, M., Angel, P., & Lichter, P. (2003). Profile of gene expression induced by the tumour promotor TPA in murine epithelial cells. INT J CANCER, 104(6), 699-708. https://doi.org/10.1002/ijc.11008

Vancouver

Schlingemann J, Hess J, Wrobel G, Breitenbach U, Gebhardt C, Steinlein P et al. Profile of gene expression induced by the tumour promotor TPA in murine epithelial cells. INT J CANCER. 2003 Mai 10;104(6):699-708. https://doi.org/10.1002/ijc.11008

Bibtex

@article{0e6508b1effd44f0854e14e525a65ce1,
title = "Profile of gene expression induced by the tumour promotor TPA in murine epithelial cells",
abstract = "Malignant transformation of mouse skin by chemical carcinogens and tumour promoters, such as the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), is a multistage process that leads to squamous cell carcinoma (SCC) formation. In an effort to identify tumour-associated genes, we studied the influence of short-term TPA-treatment on the gene expression profile of murine skin. A comprehensive microarray with some 5,000 murine gene specific cDNA fragments was established and hybridised with pooled RNA derived from control and TPA-treated dorsal skin samples. Of these genes, 54 were up- and 35 were down-regulated upon TPA application. Additionally, we performed suppression subtractive hybridisation (SSH) with respective RNA pools to generate and analyse a cDNA library enriched for TPA-inducible genes. Expression data of selected genes were confirmed by quantitative real-time PCR and Northern blot analysis. Comparison of microarray and SSH data revealed that 26% of up-regulated genes identified by expression profiling matched with those present in the SSH library. Besides numerous known genes, we identified a large set of unknown cDNAs that represent previously unrecognised TPA-regulated genes in murine skin with potential function in tumour promotion. Additionally, some TPA-induced genes, such as Sprr1A, Saa3, JunB, Il4ralpha, Gp38, RalGDS and Slpi exhibit high basal level in advanced stages of skin carcinogenesis, suggesting that at least a subgroup of the identified TPA-regulated genes may contribute to tumour progression and metastasis.",
keywords = "Animals, Biomarkers, Tumor, Blotting, Northern, Carcinogens, DNA Primers, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Gene Library, In Situ Hybridization, Keratinocytes, Male, Mice, Mice, Inbred C57BL, Neoplasm Proteins, Nucleic Acid Hybridization, Polymerase Chain Reaction, RNA, Neoplasm, Skin, Skin Neoplasms, Subtraction Technique, Tetradecanoylphorbol Acetate, Journal Article, Research Support, Non-U.S. Gov't",
author = "Joerg Schlingemann and Jochen Hess and Gunnar Wrobel and Ute Breitenbach and Christoffer Gebhardt and Peter Steinlein and Heidi Kramer and Gerhard F{\"u}rstenberger and Meinhard Hahn and Peter Angel and Peter Lichter",
note = "Copyright 2003 Wiley-Liss, Inc.",
year = "2003",
month = may,
day = "10",
doi = "10.1002/ijc.11008",
language = "English",
volume = "104",
pages = "699--708",
journal = "INT J CANCER",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "6",

}

RIS

TY - JOUR

T1 - Profile of gene expression induced by the tumour promotor TPA in murine epithelial cells

AU - Schlingemann, Joerg

AU - Hess, Jochen

AU - Wrobel, Gunnar

AU - Breitenbach, Ute

AU - Gebhardt, Christoffer

AU - Steinlein, Peter

AU - Kramer, Heidi

AU - Fürstenberger, Gerhard

AU - Hahn, Meinhard

AU - Angel, Peter

AU - Lichter, Peter

N1 - Copyright 2003 Wiley-Liss, Inc.

PY - 2003/5/10

Y1 - 2003/5/10

N2 - Malignant transformation of mouse skin by chemical carcinogens and tumour promoters, such as the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), is a multistage process that leads to squamous cell carcinoma (SCC) formation. In an effort to identify tumour-associated genes, we studied the influence of short-term TPA-treatment on the gene expression profile of murine skin. A comprehensive microarray with some 5,000 murine gene specific cDNA fragments was established and hybridised with pooled RNA derived from control and TPA-treated dorsal skin samples. Of these genes, 54 were up- and 35 were down-regulated upon TPA application. Additionally, we performed suppression subtractive hybridisation (SSH) with respective RNA pools to generate and analyse a cDNA library enriched for TPA-inducible genes. Expression data of selected genes were confirmed by quantitative real-time PCR and Northern blot analysis. Comparison of microarray and SSH data revealed that 26% of up-regulated genes identified by expression profiling matched with those present in the SSH library. Besides numerous known genes, we identified a large set of unknown cDNAs that represent previously unrecognised TPA-regulated genes in murine skin with potential function in tumour promotion. Additionally, some TPA-induced genes, such as Sprr1A, Saa3, JunB, Il4ralpha, Gp38, RalGDS and Slpi exhibit high basal level in advanced stages of skin carcinogenesis, suggesting that at least a subgroup of the identified TPA-regulated genes may contribute to tumour progression and metastasis.

AB - Malignant transformation of mouse skin by chemical carcinogens and tumour promoters, such as the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), is a multistage process that leads to squamous cell carcinoma (SCC) formation. In an effort to identify tumour-associated genes, we studied the influence of short-term TPA-treatment on the gene expression profile of murine skin. A comprehensive microarray with some 5,000 murine gene specific cDNA fragments was established and hybridised with pooled RNA derived from control and TPA-treated dorsal skin samples. Of these genes, 54 were up- and 35 were down-regulated upon TPA application. Additionally, we performed suppression subtractive hybridisation (SSH) with respective RNA pools to generate and analyse a cDNA library enriched for TPA-inducible genes. Expression data of selected genes were confirmed by quantitative real-time PCR and Northern blot analysis. Comparison of microarray and SSH data revealed that 26% of up-regulated genes identified by expression profiling matched with those present in the SSH library. Besides numerous known genes, we identified a large set of unknown cDNAs that represent previously unrecognised TPA-regulated genes in murine skin with potential function in tumour promotion. Additionally, some TPA-induced genes, such as Sprr1A, Saa3, JunB, Il4ralpha, Gp38, RalGDS and Slpi exhibit high basal level in advanced stages of skin carcinogenesis, suggesting that at least a subgroup of the identified TPA-regulated genes may contribute to tumour progression and metastasis.

KW - Animals

KW - Biomarkers, Tumor

KW - Blotting, Northern

KW - Carcinogens

KW - DNA Primers

KW - Female

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Neoplastic

KW - Gene Library

KW - In Situ Hybridization

KW - Keratinocytes

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Neoplasm Proteins

KW - Nucleic Acid Hybridization

KW - Polymerase Chain Reaction

KW - RNA, Neoplasm

KW - Skin

KW - Skin Neoplasms

KW - Subtraction Technique

KW - Tetradecanoylphorbol Acetate

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1002/ijc.11008

DO - 10.1002/ijc.11008

M3 - SCORING: Journal article

C2 - 12640676

VL - 104

SP - 699

EP - 708

JO - INT J CANCER

JF - INT J CANCER

SN - 0020-7136

IS - 6

ER -