Pro-angiogenic CD14(++) CD16(+) CD163(+) monocytes accelerate the in vitro endothelialization of soft hydrophobic poly (n-butyl acrylate) networks.
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Pro-angiogenic CD14(++) CD16(+) CD163(+) monocytes accelerate the in vitro endothelialization of soft hydrophobic poly (n-butyl acrylate) networks. / Mayer, Anke; Roch, Toralf; Kratz, Karl; Lendlein, Andreas; Jung, Friedrich.
in: ACTA BIOMATER, Jahrgang 8, Nr. 12, 12, 2012, S. 4253-4259.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Pro-angiogenic CD14(++) CD16(+) CD163(+) monocytes accelerate the in vitro endothelialization of soft hydrophobic poly (n-butyl acrylate) networks.
AU - Mayer, Anke
AU - Roch, Toralf
AU - Kratz, Karl
AU - Lendlein, Andreas
AU - Jung, Friedrich
PY - 2012
Y1 - 2012
N2 - As the majority of the polymers used as cardiovascular grafts so far do not match the elasticity of human arteries (100-1000kPa) and the required endothelialization, a multifunctional material approach is needed to allow the adjustment of the mechanical properties while at the same time exhibiting a haemocompatible surface. Recently soft poly(n-butyl acrylate) networks (cPnBA) with adjustable mechanical properties were introduced as candidate materials with a surface that can be endothelialized. In this study, angiogenically stimulated intermediate CD163(+) monocytes/macrophages (aMO2) were utilized as a cellular cytokine release system to realize the functional endothelialization of the hydrophobic cPnBA surface. We investigated the influence of co-cultured aMO2 on the morphology, density and cytokine secretion of human umbilical venous endothelial cells (HUVEC) seeded on cPnBA with an elastic modulus of around 250kPa (cPnBA0250). A functional confluent HUVEC monolayer could be developed in the co-culture within 3days. In contrast, the HUVEC in the monoculture exhibited stress fibres, broadened marginal filament bands and significantly more and larger cell-free areas in the monolayer, indicating incomplete cell-substrate binding. Remarkably, a functional confluent monolayer formation could only be achieved in co-cultures; it did not develop with the sole supplementation of recombinant VEGF-A(165) to the HUVEC monocultures (unpublished data). The study demonstrated the multifunctional potential of cPnBA in combination with aMO2 as a cellular cytokine release system, adapting their secretion to the demand of HUVEC. In this way, a functional confluent monolayer could be generated within 3days.
AB - As the majority of the polymers used as cardiovascular grafts so far do not match the elasticity of human arteries (100-1000kPa) and the required endothelialization, a multifunctional material approach is needed to allow the adjustment of the mechanical properties while at the same time exhibiting a haemocompatible surface. Recently soft poly(n-butyl acrylate) networks (cPnBA) with adjustable mechanical properties were introduced as candidate materials with a surface that can be endothelialized. In this study, angiogenically stimulated intermediate CD163(+) monocytes/macrophages (aMO2) were utilized as a cellular cytokine release system to realize the functional endothelialization of the hydrophobic cPnBA surface. We investigated the influence of co-cultured aMO2 on the morphology, density and cytokine secretion of human umbilical venous endothelial cells (HUVEC) seeded on cPnBA with an elastic modulus of around 250kPa (cPnBA0250). A functional confluent HUVEC monolayer could be developed in the co-culture within 3days. In contrast, the HUVEC in the monoculture exhibited stress fibres, broadened marginal filament bands and significantly more and larger cell-free areas in the monolayer, indicating incomplete cell-substrate binding. Remarkably, a functional confluent monolayer formation could only be achieved in co-cultures; it did not develop with the sole supplementation of recombinant VEGF-A(165) to the HUVEC monocultures (unpublished data). The study demonstrated the multifunctional potential of cPnBA in combination with aMO2 as a cellular cytokine release system, adapting their secretion to the demand of HUVEC. In this way, a functional confluent monolayer could be generated within 3days.
KW - Humans
KW - Male
KW - Female
KW - Cells, Cultured
KW - Coculture Techniques
KW - GPI-Linked Proteins
KW - Hydrophobic and Hydrophilic Interactions
KW - Neovascularization, Physiologic/drug effects
KW - Recombinant Proteins/pharmacology
KW - Antigens, CD
KW - Acrylates/pharmacology
KW - Antigens, CD14
KW - Antigens, Differentiation, Myelomonocytic
KW - Human Umbilical Vein Endothelial Cells/cytology/metabolism
KW - Macrophages/cytology/metabolism
KW - Monocytes/cytology/metabolism
KW - Receptors, Cell Surface
KW - Receptors, IgG
KW - Vascular Endothelial Growth Factor A/pharmacology
KW - Humans
KW - Male
KW - Female
KW - Cells, Cultured
KW - Coculture Techniques
KW - GPI-Linked Proteins
KW - Hydrophobic and Hydrophilic Interactions
KW - Neovascularization, Physiologic/drug effects
KW - Recombinant Proteins/pharmacology
KW - Antigens, CD
KW - Acrylates/pharmacology
KW - Antigens, CD14
KW - Antigens, Differentiation, Myelomonocytic
KW - Human Umbilical Vein Endothelial Cells/cytology/metabolism
KW - Macrophages/cytology/metabolism
KW - Monocytes/cytology/metabolism
KW - Receptors, Cell Surface
KW - Receptors, IgG
KW - Vascular Endothelial Growth Factor A/pharmacology
M3 - SCORING: Journal article
VL - 8
SP - 4253
EP - 4259
JO - ACTA BIOMATER
JF - ACTA BIOMATER
SN - 1742-7061
IS - 12
M1 - 12
ER -