Presence of adenovirus species C in infiltrating lymphocytes of human sarcoma
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Presence of adenovirus species C in infiltrating lymphocytes of human sarcoma. / Kosulin, Karin; Hoffmann, Franziska; Clauditz, Till Sebastian; Wilczak, Waldemar; Dobner, Thomas.
in: PLOS ONE, Jahrgang 8, Nr. 5, 01.01.2013, S. e63646.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Presence of adenovirus species C in infiltrating lymphocytes of human sarcoma
AU - Kosulin, Karin
AU - Hoffmann, Franziska
AU - Clauditz, Till Sebastian
AU - Wilczak, Waldemar
AU - Dobner, Thomas
PY - 2013/1/1
Y1 - 2013/1/1
N2 - Human adenoviruses are known to persist in T-lymphocytes of tonsils, adenoids and intestinal tract. The oncogenic potential of different adenovirus types has been widely studied in rodents, in which adenovirus inoculation can induce multiple tumors such as undifferentiated sarcomas, adenocarcinomas and neuroectodermal tumors. However, the oncogenic potential of this virus has never been proven in human subjects. Using a highly sensitive broad-spectrum qRT-PCR, we have screened a set of different human sarcomas including leiomyosarcoma, liposarcoma and gastro intestinal stroma tumors. Primers binding the viral oncogene E1A and the capsid-coding gene Hexon were used to detect the presence of adenovirus DNA in tumor samples. We found that 18% of the tested leiomyosarcomas and 35% of the liposarcomas were positive for the presence of adenovirus DNA, being species C types the most frequently detected adenoviruses. However, only in one sample of the gastro intestinal stroma tumors the virus DNA could be detected. The occurrence of adenovirus in the tumor sections was confirmed by subsequent fluorescence in-situ-hybridization analysis and co-staining with the transcription factor Bcl11b gives evidence for the presence of the virus in infiltrating T-lymphocytes within the tumors. Together these data underline, for the first time, the persistence of adenovirus in T-lymphocytes infiltrated in muscular and fatty tissue tumor samples. If an impaired immune system leads to the viral persistence and reactivation of the virus is involved in additional diseases needs further investigation.
AB - Human adenoviruses are known to persist in T-lymphocytes of tonsils, adenoids and intestinal tract. The oncogenic potential of different adenovirus types has been widely studied in rodents, in which adenovirus inoculation can induce multiple tumors such as undifferentiated sarcomas, adenocarcinomas and neuroectodermal tumors. However, the oncogenic potential of this virus has never been proven in human subjects. Using a highly sensitive broad-spectrum qRT-PCR, we have screened a set of different human sarcomas including leiomyosarcoma, liposarcoma and gastro intestinal stroma tumors. Primers binding the viral oncogene E1A and the capsid-coding gene Hexon were used to detect the presence of adenovirus DNA in tumor samples. We found that 18% of the tested leiomyosarcomas and 35% of the liposarcomas were positive for the presence of adenovirus DNA, being species C types the most frequently detected adenoviruses. However, only in one sample of the gastro intestinal stroma tumors the virus DNA could be detected. The occurrence of adenovirus in the tumor sections was confirmed by subsequent fluorescence in-situ-hybridization analysis and co-staining with the transcription factor Bcl11b gives evidence for the presence of the virus in infiltrating T-lymphocytes within the tumors. Together these data underline, for the first time, the persistence of adenovirus in T-lymphocytes infiltrated in muscular and fatty tissue tumor samples. If an impaired immune system leads to the viral persistence and reactivation of the virus is involved in additional diseases needs further investigation.
KW - Adenovirus E1A Proteins
KW - Adenoviruses, Human
KW - Base Sequence
KW - Host-Pathogen Interactions
KW - Humans
KW - In Situ Hybridization, Fluorescence
KW - Leiomyosarcoma
KW - Liposarcoma
KW - Molecular Diagnostic Techniques
KW - Molecular Sequence Data
KW - Molecular Typing
KW - Real-Time Polymerase Chain Reaction
KW - Sequence Analysis, DNA
KW - T-Lymphocytes
KW - Viral Load
U2 - 10.1371/journal.pone.0063646
DO - 10.1371/journal.pone.0063646
M3 - SCORING: Journal article
C2 - 23671688
VL - 8
SP - e63646
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 5
ER -