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Preferential upregulation of interferon-alpha subtype 2 expression in HIV-1 patients.

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Preferential upregulation of interferon-alpha subtype 2 expression in HIV-1 patients. / Lehmann, C; Taubert, D; Jung, N; Fätkenheuer, G; van Lunzen, Jan; Hartmann, P; Romerio, F.

in: AIDS RES HUM RETROV, Jahrgang 25, Nr. 6, 6, 2009, S. 577-581.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Lehmann, C, Taubert, D, Jung, N, Fätkenheuer, G, van Lunzen, J, Hartmann, P & Romerio, F 2009, 'Preferential upregulation of interferon-alpha subtype 2 expression in HIV-1 patients.', AIDS RES HUM RETROV, Jg. 25, Nr. 6, 6, S. 577-581. <http://www.ncbi.nlm.nih.gov/pubmed/19500019?dopt=Citation>

APA

Lehmann, C., Taubert, D., Jung, N., Fätkenheuer, G., van Lunzen, J., Hartmann, P., & Romerio, F. (2009). Preferential upregulation of interferon-alpha subtype 2 expression in HIV-1 patients. AIDS RES HUM RETROV, 25(6), 577-581. [6]. http://www.ncbi.nlm.nih.gov/pubmed/19500019?dopt=Citation

Vancouver

Lehmann C, Taubert D, Jung N, Fätkenheuer G, van Lunzen J, Hartmann P et al. Preferential upregulation of interferon-alpha subtype 2 expression in HIV-1 patients. AIDS RES HUM RETROV. 2009;25(6):577-581. 6.

Bibtex

@article{763261fc6263452a8dd276a45371995c,
title = "Preferential upregulation of interferon-alpha subtype 2 expression in HIV-1 patients.",
abstract = "Humans tailor virus-specific immune responses through modulated expression of 12 different interferon (IFN)-alpha subtypes. However, exacerbated expression of certain IFN-alpha subtypes causes immunopathology in the context of autoimmune conditions and chronic viral infections. We showed that progression to AIDS is associated with elevated expression of IFN-alpha in unstimulated peripheral blood mononuclear cells. Here, we sought to determine whether distinct IFN-alpha subtypes are involved in this phenomenon. We used quantitative RT-PCR to assess expression levels of 12 IFN-alpha subtypes in peripheral blood mononuclear cells from normal donors and HIV-1 patients at CDC stage A and stage C of the disease. Three patterns of IFN-alpha subtype expression emerged. First, IFN-alpha2 and IFN-alpha6 mRNA levels were elevated in both patient groups. Second, IFN-alpha1/13, IFN-alpha8, IFN-alpha14, IFN-alpha16, IFN-alpha17, and IFN-alpha21 were upregulated in stage C but not stage A patients. Third, expression levels of IFN-alpha4, IFN-alpha5, IFN-alpha7, and IFN-alpha10 did not change among the three groups of volunteers. Among all other subtypes, IFN-alpha2 was preferentially upregulated, showing >60-fold higher levels in stage A and >400-fold in stage C patients compared with controls, which correlated with declining CD4 counts. Our results demonstrate that distinct IFN-alpha subtypes are sequentially activated during HIV-1 infection, which may be predictive of disease progression.",
keywords = "Adult, Humans, Male, Female, Middle Aged, Cells, Cultured, Up-Regulation, Gene Expression Profiling methods, HIV Infections immunology, HIV-1 immunology, Interferon-alpha biosynthesis, Leukocytes, Mononuclear immunology, Reverse Transcriptase Polymerase Chain Reaction methods, Adult, Humans, Male, Female, Middle Aged, Cells, Cultured, Up-Regulation, Gene Expression Profiling methods, HIV Infections immunology, HIV-1 immunology, Interferon-alpha biosynthesis, Leukocytes, Mononuclear immunology, Reverse Transcriptase Polymerase Chain Reaction methods",
author = "C Lehmann and D Taubert and N Jung and G F{\"a}tkenheuer and {van Lunzen}, Jan and P Hartmann and F Romerio",
year = "2009",
language = "Deutsch",
volume = "25",
pages = "577--581",
journal = "AIDS RES HUM RETROV",
issn = "0889-2229",
publisher = "Mary Ann Liebert Inc.",
number = "6",

}

RIS

TY - JOUR

T1 - Preferential upregulation of interferon-alpha subtype 2 expression in HIV-1 patients.

AU - Lehmann, C

AU - Taubert, D

AU - Jung, N

AU - Fätkenheuer, G

AU - van Lunzen, Jan

AU - Hartmann, P

AU - Romerio, F

PY - 2009

Y1 - 2009

N2 - Humans tailor virus-specific immune responses through modulated expression of 12 different interferon (IFN)-alpha subtypes. However, exacerbated expression of certain IFN-alpha subtypes causes immunopathology in the context of autoimmune conditions and chronic viral infections. We showed that progression to AIDS is associated with elevated expression of IFN-alpha in unstimulated peripheral blood mononuclear cells. Here, we sought to determine whether distinct IFN-alpha subtypes are involved in this phenomenon. We used quantitative RT-PCR to assess expression levels of 12 IFN-alpha subtypes in peripheral blood mononuclear cells from normal donors and HIV-1 patients at CDC stage A and stage C of the disease. Three patterns of IFN-alpha subtype expression emerged. First, IFN-alpha2 and IFN-alpha6 mRNA levels were elevated in both patient groups. Second, IFN-alpha1/13, IFN-alpha8, IFN-alpha14, IFN-alpha16, IFN-alpha17, and IFN-alpha21 were upregulated in stage C but not stage A patients. Third, expression levels of IFN-alpha4, IFN-alpha5, IFN-alpha7, and IFN-alpha10 did not change among the three groups of volunteers. Among all other subtypes, IFN-alpha2 was preferentially upregulated, showing >60-fold higher levels in stage A and >400-fold in stage C patients compared with controls, which correlated with declining CD4 counts. Our results demonstrate that distinct IFN-alpha subtypes are sequentially activated during HIV-1 infection, which may be predictive of disease progression.

AB - Humans tailor virus-specific immune responses through modulated expression of 12 different interferon (IFN)-alpha subtypes. However, exacerbated expression of certain IFN-alpha subtypes causes immunopathology in the context of autoimmune conditions and chronic viral infections. We showed that progression to AIDS is associated with elevated expression of IFN-alpha in unstimulated peripheral blood mononuclear cells. Here, we sought to determine whether distinct IFN-alpha subtypes are involved in this phenomenon. We used quantitative RT-PCR to assess expression levels of 12 IFN-alpha subtypes in peripheral blood mononuclear cells from normal donors and HIV-1 patients at CDC stage A and stage C of the disease. Three patterns of IFN-alpha subtype expression emerged. First, IFN-alpha2 and IFN-alpha6 mRNA levels were elevated in both patient groups. Second, IFN-alpha1/13, IFN-alpha8, IFN-alpha14, IFN-alpha16, IFN-alpha17, and IFN-alpha21 were upregulated in stage C but not stage A patients. Third, expression levels of IFN-alpha4, IFN-alpha5, IFN-alpha7, and IFN-alpha10 did not change among the three groups of volunteers. Among all other subtypes, IFN-alpha2 was preferentially upregulated, showing >60-fold higher levels in stage A and >400-fold in stage C patients compared with controls, which correlated with declining CD4 counts. Our results demonstrate that distinct IFN-alpha subtypes are sequentially activated during HIV-1 infection, which may be predictive of disease progression.

KW - Adult

KW - Humans

KW - Male

KW - Female

KW - Middle Aged

KW - Cells, Cultured

KW - Up-Regulation

KW - Gene Expression Profiling methods

KW - HIV Infections immunology

KW - HIV-1 immunology

KW - Interferon-alpha biosynthesis

KW - Leukocytes, Mononuclear immunology

KW - Reverse Transcriptase Polymerase Chain Reaction methods

KW - Adult

KW - Humans

KW - Male

KW - Female

KW - Middle Aged

KW - Cells, Cultured

KW - Up-Regulation

KW - Gene Expression Profiling methods

KW - HIV Infections immunology

KW - HIV-1 immunology

KW - Interferon-alpha biosynthesis

KW - Leukocytes, Mononuclear immunology

KW - Reverse Transcriptase Polymerase Chain Reaction methods

M3 - SCORING: Zeitschriftenaufsatz

VL - 25

SP - 577

EP - 581

JO - AIDS RES HUM RETROV

JF - AIDS RES HUM RETROV

SN - 0889-2229

IS - 6

M1 - 6

ER -