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Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions

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Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions. / Fornasiero, Eugenio F; Mandad, Sunit; Wildhagen, Hanna; Alevra, Mihai; Rammner, Burkhard; Keihani, Sarva; Opazo, Felipe; Urban, Inga; Ischebeck, Till; Sakib, M Sadman; Fard, Maryam K; Kirli, Koray; Centeno, Tonatiuh Pena; Vidal, Ramon O; Rahman, Raza-Ur; Benito, Eva; Fischer, André; Dennerlein, Sven; Rehling, Peter; Feussner, Ivo; Bonn, Stefan; Simons, Mikael; Urlaub, Henning; Rizzoli, Silvio O.

in: NAT COMMUN, Jahrgang 9, Nr. 1, 12.10.2018, S. 4230.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Fornasiero, EF, Mandad, S, Wildhagen, H, Alevra, M, Rammner, B, Keihani, S, Opazo, F, Urban, I, Ischebeck, T, Sakib, MS, Fard, MK, Kirli, K, Centeno, TP, Vidal, RO, Rahman, R-U, Benito, E, Fischer, A, Dennerlein, S, Rehling, P, Feussner, I, Bonn, S, Simons, M, Urlaub, H & Rizzoli, SO 2018, 'Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions', NAT COMMUN, Jg. 9, Nr. 1, S. 4230. https://doi.org/10.1038/s41467-018-06519-0

APA

Fornasiero, E. F., Mandad, S., Wildhagen, H., Alevra, M., Rammner, B., Keihani, S., Opazo, F., Urban, I., Ischebeck, T., Sakib, M. S., Fard, M. K., Kirli, K., Centeno, T. P., Vidal, R. O., Rahman, R-U., Benito, E., Fischer, A., Dennerlein, S., Rehling, P., ... Rizzoli, S. O. (2018). Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions. NAT COMMUN, 9(1), 4230. https://doi.org/10.1038/s41467-018-06519-0

Vancouver

Fornasiero EF, Mandad S, Wildhagen H, Alevra M, Rammner B, Keihani S et al. Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions. NAT COMMUN. 2018 Okt 12;9(1):4230. https://doi.org/10.1038/s41467-018-06519-0

Bibtex

@article{a9955a233a314c92b5be7449b10db937,
title = "Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions",
abstract = "The turnover of brain proteins is critical for organism survival, and its perturbations are linked to pathology. Nevertheless, protein lifetimes have been difficult to obtain in vivo. They are readily measured in vitro by feeding cells with isotopically labeled amino acids, followed by mass spectrometry analyses. In vivo proteins are generated from at least two sources: labeled amino acids from the diet, and non-labeled amino acids from the degradation of pre-existing proteins. This renders measurements difficult. Here we solved this problem rigorously with a workflow that combines mouse in vivo isotopic labeling, mass spectrometry, and mathematical modeling. We also established several independent approaches to test and validate the results. This enabled us to measure the accurate lifetimes of ~3500 brain proteins. The high precision of our data provided a large set of biologically significant observations, including pathway-, organelle-, organ-, or cell-specific effects, along with a comprehensive catalog of extremely long-lived proteins (ELLPs).",
keywords = "Journal Article, Research Support, Non-U.S. Gov't",
author = "Fornasiero, {Eugenio F} and Sunit Mandad and Hanna Wildhagen and Mihai Alevra and Burkhard Rammner and Sarva Keihani and Felipe Opazo and Inga Urban and Till Ischebeck and Sakib, {M Sadman} and Fard, {Maryam K} and Koray Kirli and Centeno, {Tonatiuh Pena} and Vidal, {Ramon O} and Raza-Ur Rahman and Eva Benito and Andr{\'e} Fischer and Sven Dennerlein and Peter Rehling and Ivo Feussner and Stefan Bonn and Mikael Simons and Henning Urlaub and Rizzoli, {Silvio O}",
year = "2018",
month = oct,
day = "12",
doi = "10.1038/s41467-018-06519-0",
language = "English",
volume = "9",
pages = "4230",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions

AU - Fornasiero, Eugenio F

AU - Mandad, Sunit

AU - Wildhagen, Hanna

AU - Alevra, Mihai

AU - Rammner, Burkhard

AU - Keihani, Sarva

AU - Opazo, Felipe

AU - Urban, Inga

AU - Ischebeck, Till

AU - Sakib, M Sadman

AU - Fard, Maryam K

AU - Kirli, Koray

AU - Centeno, Tonatiuh Pena

AU - Vidal, Ramon O

AU - Rahman, Raza-Ur

AU - Benito, Eva

AU - Fischer, André

AU - Dennerlein, Sven

AU - Rehling, Peter

AU - Feussner, Ivo

AU - Bonn, Stefan

AU - Simons, Mikael

AU - Urlaub, Henning

AU - Rizzoli, Silvio O

PY - 2018/10/12

Y1 - 2018/10/12

N2 - The turnover of brain proteins is critical for organism survival, and its perturbations are linked to pathology. Nevertheless, protein lifetimes have been difficult to obtain in vivo. They are readily measured in vitro by feeding cells with isotopically labeled amino acids, followed by mass spectrometry analyses. In vivo proteins are generated from at least two sources: labeled amino acids from the diet, and non-labeled amino acids from the degradation of pre-existing proteins. This renders measurements difficult. Here we solved this problem rigorously with a workflow that combines mouse in vivo isotopic labeling, mass spectrometry, and mathematical modeling. We also established several independent approaches to test and validate the results. This enabled us to measure the accurate lifetimes of ~3500 brain proteins. The high precision of our data provided a large set of biologically significant observations, including pathway-, organelle-, organ-, or cell-specific effects, along with a comprehensive catalog of extremely long-lived proteins (ELLPs).

AB - The turnover of brain proteins is critical for organism survival, and its perturbations are linked to pathology. Nevertheless, protein lifetimes have been difficult to obtain in vivo. They are readily measured in vitro by feeding cells with isotopically labeled amino acids, followed by mass spectrometry analyses. In vivo proteins are generated from at least two sources: labeled amino acids from the diet, and non-labeled amino acids from the degradation of pre-existing proteins. This renders measurements difficult. Here we solved this problem rigorously with a workflow that combines mouse in vivo isotopic labeling, mass spectrometry, and mathematical modeling. We also established several independent approaches to test and validate the results. This enabled us to measure the accurate lifetimes of ~3500 brain proteins. The high precision of our data provided a large set of biologically significant observations, including pathway-, organelle-, organ-, or cell-specific effects, along with a comprehensive catalog of extremely long-lived proteins (ELLPs).

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1038/s41467-018-06519-0

DO - 10.1038/s41467-018-06519-0

M3 - SCORING: Journal article

C2 - 30315172

VL - 9

SP - 4230

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

IS - 1

ER -