Poor correlation between BCG vaccination-induced T cell responses and protection against tuberculosis.
Standard
Poor correlation between BCG vaccination-induced T cell responses and protection against tuberculosis. / Mittrücker, Hans Willi; Steinhoff, Ulrich; Köhler, Anne; Krause, Marion; Lazar, Doris; Mex, Peggy; Miekley, Delia; Kaufmann, Stefan H E.
in: P NATL ACAD SCI USA, Jahrgang 104, Nr. 30, 30, 2007, S. 12434-12439.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Poor correlation between BCG vaccination-induced T cell responses and protection against tuberculosis.
AU - Mittrücker, Hans Willi
AU - Steinhoff, Ulrich
AU - Köhler, Anne
AU - Krause, Marion
AU - Lazar, Doris
AU - Mex, Peggy
AU - Miekley, Delia
AU - Kaufmann, Stefan H E
PY - 2007
Y1 - 2007
N2 - Mycobacterium bovis bacille Calmette-Guérin (BCG) is the most widely used live bacterial vaccine. However, limited information is available correlating route and dose of vaccination and induction of specific T cell responses with protection against tuberculosis. We compared efficacy of oral and systemic vaccination and correlated vaccine-induced T cell responses with protection in experimental tuberculosis of mice. After oral and systemic vaccination, we observed profound differences in persistence and dissemination of BCG and frequencies and location of specific IFN-gamma-secreting CD4(+) and CD8(+) T cells. Yet, both vaccination routes caused comparable levels of protection against aerosol challenge with Mycobacterium tuberculosis. Protection correlated best with rapid accumulation of specific CD8(+) T cells in infected tissues of challenged mice. In contrast, specific IFN-gamma production by CD4(+) T cells reflected the load of M. tuberculosis rather than the strength of protection. Our data question the measurement of IFN-gamma secretion by CD4(+) T cells and emphasize the need for new biomarkers for evaluation of tuberculosis vaccine efficacies.
AB - Mycobacterium bovis bacille Calmette-Guérin (BCG) is the most widely used live bacterial vaccine. However, limited information is available correlating route and dose of vaccination and induction of specific T cell responses with protection against tuberculosis. We compared efficacy of oral and systemic vaccination and correlated vaccine-induced T cell responses with protection in experimental tuberculosis of mice. After oral and systemic vaccination, we observed profound differences in persistence and dissemination of BCG and frequencies and location of specific IFN-gamma-secreting CD4(+) and CD8(+) T cells. Yet, both vaccination routes caused comparable levels of protection against aerosol challenge with Mycobacterium tuberculosis. Protection correlated best with rapid accumulation of specific CD8(+) T cells in infected tissues of challenged mice. In contrast, specific IFN-gamma production by CD4(+) T cells reflected the load of M. tuberculosis rather than the strength of protection. Our data question the measurement of IFN-gamma secretion by CD4(+) T cells and emphasize the need for new biomarkers for evaluation of tuberculosis vaccine efficacies.
M3 - SCORING: Zeitschriftenaufsatz
VL - 104
SP - 12434
EP - 12439
JO - P NATL ACAD SCI USA
JF - P NATL ACAD SCI USA
SN - 0027-8424
IS - 30
M1 - 30
ER -
