Photofunctionalization and non-thermal plasma activation of titanium surfaces
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Photofunctionalization and non-thermal plasma activation of titanium surfaces. / Henningsen, Anders; Smeets, Ralf; Hartjen, Philip; Heinrich, Oliver; Heuberger, Roman; Heiland, Max; Precht, Clarissa; Cacaci, Claudio.
in: CLIN ORAL INVEST, Jahrgang 22, Nr. 2, 03.2018, S. 1045-1054.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Photofunctionalization and non-thermal plasma activation of titanium surfaces
AU - Henningsen, Anders
AU - Smeets, Ralf
AU - Hartjen, Philip
AU - Heinrich, Oliver
AU - Heuberger, Roman
AU - Heiland, Max
AU - Precht, Clarissa
AU - Cacaci, Claudio
PY - 2018/3
Y1 - 2018/3
N2 - OBJECTIVE: The aim of this study was to compare UV light and non-thermal plasma (NTP) treatment regarding the improvement of physical material characteristics and cell reaction on titanium surfaces in vitro after short-term functionalization.MATERIALS AND METHODS: Moderately rough (Ra 1.8-2.0 μm) sandblasted and acid-etched titanium disks were treated by UV light (0.05 mW/cm(2) at λ = 360 nm and 2 mW/cm(2) at λ = 250 nm) or by NTP (24 W, -0.5 mbar) of argon or oxygen for 12 min each. Surface structure was investigated by scanning electron microscopy, confocal microscopy and X-ray photoelectron spectroscopy (XPS). Hydrophilicity was assessed by dynamic contact angle measurement. Cell attachment, viability, cell proliferation and cytotoxicity were assessed in vitro using murine osteoblast-like cells.RESULTS: UV irradiation or NTP treatment of titanium surfaces did not alter the surface structure. XPS analysis revealed a significantly increased oxidation of the surface and a decrease of carbon after the use of either method. NTP and UV light led to a significant better cell attachment of murine osteoblasts; significantly more osteoblasts grew on the treated surfaces at each time point (p < 0.001).CONCLUSIONS: UV light as well as NTP modified the surface of titanium and significantly improved the conditions for murine osteoblast cells in vitro. However, results indicate a slight advantage for NTP of argon and oxygen in a short time interval of surface functionalization compared to UV.CLINICAL RELEVANCE: UV light and NTP are able to improve surface conditions of dental implants made of titanium.
AB - OBJECTIVE: The aim of this study was to compare UV light and non-thermal plasma (NTP) treatment regarding the improvement of physical material characteristics and cell reaction on titanium surfaces in vitro after short-term functionalization.MATERIALS AND METHODS: Moderately rough (Ra 1.8-2.0 μm) sandblasted and acid-etched titanium disks were treated by UV light (0.05 mW/cm(2) at λ = 360 nm and 2 mW/cm(2) at λ = 250 nm) or by NTP (24 W, -0.5 mbar) of argon or oxygen for 12 min each. Surface structure was investigated by scanning electron microscopy, confocal microscopy and X-ray photoelectron spectroscopy (XPS). Hydrophilicity was assessed by dynamic contact angle measurement. Cell attachment, viability, cell proliferation and cytotoxicity were assessed in vitro using murine osteoblast-like cells.RESULTS: UV irradiation or NTP treatment of titanium surfaces did not alter the surface structure. XPS analysis revealed a significantly increased oxidation of the surface and a decrease of carbon after the use of either method. NTP and UV light led to a significant better cell attachment of murine osteoblasts; significantly more osteoblasts grew on the treated surfaces at each time point (p < 0.001).CONCLUSIONS: UV light as well as NTP modified the surface of titanium and significantly improved the conditions for murine osteoblast cells in vitro. However, results indicate a slight advantage for NTP of argon and oxygen in a short time interval of surface functionalization compared to UV.CLINICAL RELEVANCE: UV light and NTP are able to improve surface conditions of dental implants made of titanium.
KW - Journal Article
U2 - 10.1007/s00784-017-2186-z
DO - 10.1007/s00784-017-2186-z
M3 - SCORING: Journal article
C2 - 28730456
VL - 22
SP - 1045
EP - 1054
JO - CLIN ORAL INVEST
JF - CLIN ORAL INVEST
SN - 1432-6981
IS - 2
ER -