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Performance of a loop-mediated isothermal amplification assay (Isoplex CRE-ART) to detect common carbapenemase-encoding genes in Gram-negative bacteria

Standard

Performance of a loop-mediated isothermal amplification assay (Isoplex CRE-ART) to detect common carbapenemase-encoding genes in Gram-negative bacteria. / Berneking, Laura; Asar, Lucia; Both, Anna; Berinson, Benjamin; Aepfelbacher, Martin; Lütgehetmann, Marc; Rohde, Holger.

in: J MED MICROBIOL, Jahrgang 70, Nr. 7, 07.2021.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Berneking, L, Asar, L, Both, A, Berinson, B, Aepfelbacher, M, Lütgehetmann, M & Rohde, H 2021, 'Performance of a loop-mediated isothermal amplification assay (Isoplex CRE-ART) to detect common carbapenemase-encoding genes in Gram-negative bacteria', J MED MICROBIOL, Jg. 70, Nr. 7. https://doi.org/10.1099/jmm.0.001379

APA

Berneking, L., Asar, L., Both, A., Berinson, B., Aepfelbacher, M., Lütgehetmann, M., & Rohde, H. (2021). Performance of a loop-mediated isothermal amplification assay (Isoplex CRE-ART) to detect common carbapenemase-encoding genes in Gram-negative bacteria. J MED MICROBIOL, 70(7). https://doi.org/10.1099/jmm.0.001379

Vancouver

Berneking L, Asar L, Both A, Berinson B, Aepfelbacher M, Lütgehetmann M et al. Performance of a loop-mediated isothermal amplification assay (Isoplex CRE-ART) to detect common carbapenemase-encoding genes in Gram-negative bacteria. J MED MICROBIOL. 2021 Jul;70(7). https://doi.org/10.1099/jmm.0.001379

Bibtex

@article{71934bdc606446d4bb46a9e2535cbe7d,
title = "Performance of a loop-mediated isothermal amplification assay (Isoplex CRE-ART) to detect common carbapenemase-encoding genes in Gram-negative bacteria",
abstract = "Carbapenem-resistant Gram-negative bacteria (CR-GNB) are a major source of nosocomial infections worldwide. In this study, the ability of a loop-mediated isothermal amplification (LAMP)-based method (Isoplex CRE-ART) to rapidly detect carbapenemase-encoding genes bla OXA-48-like, bla OXA-23-like, bla OXA-24-like, bla KPC, bla VIM, bla NDM and bla IMP in 231 carbapenem-resistant Enterobacterales, Pseudomonas aeruginosa and Acinetobacter baumannii isolates was investigated. The accuracy of the LAMP test was compared to results of molecular isolate characterization using a Laboratory Developed Test multiplex carbapenemase PCR assay. The LAMP test correctly identified the presence of on-panel carbapenemases with a sensitivity of 99.16 % [95 % confidence interval (CI): 95.39-99.96 %] and a specificity of 98.21 % (95 % CI: 93.72-99.68 %) in 60 min. Our findings suggest that the Isoplex CRE-ART assay is able to rapidly identify carbapenemase genes in CR-GNB and improves options for pathogen characterization in the context of clinical microbiological and infection control diagnostics.",
author = "Laura Berneking and Lucia Asar and Anna Both and Benjamin Berinson and Martin Aepfelbacher and Marc L{\"u}tgehetmann and Holger Rohde",
year = "2021",
month = jul,
doi = "10.1099/jmm.0.001379",
language = "English",
volume = "70",
journal = "J MED MICROBIOL",
issn = "0022-2615",
publisher = "Society for General Microbiology",
number = "7",

}

RIS

TY - JOUR

T1 - Performance of a loop-mediated isothermal amplification assay (Isoplex CRE-ART) to detect common carbapenemase-encoding genes in Gram-negative bacteria

AU - Berneking, Laura

AU - Asar, Lucia

AU - Both, Anna

AU - Berinson, Benjamin

AU - Aepfelbacher, Martin

AU - Lütgehetmann, Marc

AU - Rohde, Holger

PY - 2021/7

Y1 - 2021/7

N2 - Carbapenem-resistant Gram-negative bacteria (CR-GNB) are a major source of nosocomial infections worldwide. In this study, the ability of a loop-mediated isothermal amplification (LAMP)-based method (Isoplex CRE-ART) to rapidly detect carbapenemase-encoding genes bla OXA-48-like, bla OXA-23-like, bla OXA-24-like, bla KPC, bla VIM, bla NDM and bla IMP in 231 carbapenem-resistant Enterobacterales, Pseudomonas aeruginosa and Acinetobacter baumannii isolates was investigated. The accuracy of the LAMP test was compared to results of molecular isolate characterization using a Laboratory Developed Test multiplex carbapenemase PCR assay. The LAMP test correctly identified the presence of on-panel carbapenemases with a sensitivity of 99.16 % [95 % confidence interval (CI): 95.39-99.96 %] and a specificity of 98.21 % (95 % CI: 93.72-99.68 %) in 60 min. Our findings suggest that the Isoplex CRE-ART assay is able to rapidly identify carbapenemase genes in CR-GNB and improves options for pathogen characterization in the context of clinical microbiological and infection control diagnostics.

AB - Carbapenem-resistant Gram-negative bacteria (CR-GNB) are a major source of nosocomial infections worldwide. In this study, the ability of a loop-mediated isothermal amplification (LAMP)-based method (Isoplex CRE-ART) to rapidly detect carbapenemase-encoding genes bla OXA-48-like, bla OXA-23-like, bla OXA-24-like, bla KPC, bla VIM, bla NDM and bla IMP in 231 carbapenem-resistant Enterobacterales, Pseudomonas aeruginosa and Acinetobacter baumannii isolates was investigated. The accuracy of the LAMP test was compared to results of molecular isolate characterization using a Laboratory Developed Test multiplex carbapenemase PCR assay. The LAMP test correctly identified the presence of on-panel carbapenemases with a sensitivity of 99.16 % [95 % confidence interval (CI): 95.39-99.96 %] and a specificity of 98.21 % (95 % CI: 93.72-99.68 %) in 60 min. Our findings suggest that the Isoplex CRE-ART assay is able to rapidly identify carbapenemase genes in CR-GNB and improves options for pathogen characterization in the context of clinical microbiological and infection control diagnostics.

U2 - 10.1099/jmm.0.001379

DO - 10.1099/jmm.0.001379

M3 - SCORING: Journal article

C2 - 34251298

VL - 70

JO - J MED MICROBIOL

JF - J MED MICROBIOL

SN - 0022-2615

IS - 7

ER -