Patterns of synaptic loss in human amyotrophic lateral sclerosis spinal cord: a clinicopathological study
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Patterns of synaptic loss in human amyotrophic lateral sclerosis spinal cord: a clinicopathological study. / Aousji, Oumayma; Feldengut, Simone; Antonucci, Stefano; Schön, Michael; Boeckers, Tobias M; Matschke, Jakob; Mawrin, Christian; Ludolph, Albert C; Del Tredici, Kelly; Roselli, Francesco; Braak, Heiko.
in: ACTA NEUROPATHOL COM, Jahrgang 11, Nr. 1, 25.07.2023, S. 120.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Patterns of synaptic loss in human amyotrophic lateral sclerosis spinal cord: a clinicopathological study
AU - Aousji, Oumayma
AU - Feldengut, Simone
AU - Antonucci, Stefano
AU - Schön, Michael
AU - Boeckers, Tobias M
AU - Matschke, Jakob
AU - Mawrin, Christian
AU - Ludolph, Albert C
AU - Del Tredici, Kelly
AU - Roselli, Francesco
AU - Braak, Heiko
N1 - © 2023. The Author(s).
PY - 2023/7/25
Y1 - 2023/7/25
N2 - Amyotrophic Lateral Sclerosis (ALS) is mainly characterized by the degeneration of corticospinal neurons and spinal α-motoneurons; vulnerable cells display prominent pTDP-43 inclusions. Evidence gathered from genetics, murine models, and iPSC-derived neurons point to the early involvement of synapses in the disease course and their crucial role in the pathogenic cascade. However, pathology studies, with specimens from large post-mortem cohorts, mapping the pattern of synaptic disturbances over clinical and neuropathological hallmarks of disease progression, are currently not available. Thus, the appearance and progression of synaptic degeneration in human ALS patients are currently not known, preventing a full validation of the murine and in vitro models. Here, we investigated the loss of synaptophysin-positive terminals in cervical, thoracic, and lumbar spinal cord samples from a retrospective cohort of n = 33 ALS patients and n = 8 healthy controls, and we correlated the loss of synapses against clinicodemographic features and neuropathological ALS stage. We found that, although dorsal and intermediate spinal cord laminae do not lose synapses, ALS patients displayed a substantial but variable loss of synapses in the ventral horn of lumbar and cervical spinal cord. The amount of synaptic loss was predicted by disease duration, by the clinical site of onset, and by the loss of α-motoneurons, although not by the fraction of pTDP-43-immunopositive α-motoneurons. Taken together, our findings validate the synaptic pathology observed in other models and suggest that pathogenic pathways unfolding in the spinal microenvironment are critical to the progressive disassembly of local synaptic connectivity.
AB - Amyotrophic Lateral Sclerosis (ALS) is mainly characterized by the degeneration of corticospinal neurons and spinal α-motoneurons; vulnerable cells display prominent pTDP-43 inclusions. Evidence gathered from genetics, murine models, and iPSC-derived neurons point to the early involvement of synapses in the disease course and their crucial role in the pathogenic cascade. However, pathology studies, with specimens from large post-mortem cohorts, mapping the pattern of synaptic disturbances over clinical and neuropathological hallmarks of disease progression, are currently not available. Thus, the appearance and progression of synaptic degeneration in human ALS patients are currently not known, preventing a full validation of the murine and in vitro models. Here, we investigated the loss of synaptophysin-positive terminals in cervical, thoracic, and lumbar spinal cord samples from a retrospective cohort of n = 33 ALS patients and n = 8 healthy controls, and we correlated the loss of synapses against clinicodemographic features and neuropathological ALS stage. We found that, although dorsal and intermediate spinal cord laminae do not lose synapses, ALS patients displayed a substantial but variable loss of synapses in the ventral horn of lumbar and cervical spinal cord. The amount of synaptic loss was predicted by disease duration, by the clinical site of onset, and by the loss of α-motoneurons, although not by the fraction of pTDP-43-immunopositive α-motoneurons. Taken together, our findings validate the synaptic pathology observed in other models and suggest that pathogenic pathways unfolding in the spinal microenvironment are critical to the progressive disassembly of local synaptic connectivity.
KW - Humans
KW - Mice
KW - Animals
KW - Amyotrophic Lateral Sclerosis/pathology
KW - Retrospective Studies
KW - Motor Neurons/metabolism
KW - Spinal Cord/pathology
U2 - 10.1186/s40478-023-01616-8
DO - 10.1186/s40478-023-01616-8
M3 - SCORING: Journal article
C2 - 37491361
VL - 11
SP - 120
JO - ACTA NEUROPATHOL COM
JF - ACTA NEUROPATHOL COM
SN - 2051-5960
IS - 1
ER -