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Patterns of cell death and cell cycle profiles of cultured WEHI 13 var fibroblasts exposed to eluates of composite resins used for direct and indirect restorations

Standard

Patterns of cell death and cell cycle profiles of cultured WEHI 13 var fibroblasts exposed to eluates of composite resins used for direct and indirect restorations. / Bakopoulou, Athina; Tsiftsoglou, Asterios; Galaktidou, Grammati; Markala, Dimitra; Triviai, Ioanna; Garefis, Pavlos.

in: EUR J ORAL SCI, Jahrgang 115, Nr. 5, 10.2007, S. 397-407.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Bakopoulou, A, Tsiftsoglou, A, Galaktidou, G, Markala, D, Triviai, I & Garefis, P 2007, 'Patterns of cell death and cell cycle profiles of cultured WEHI 13 var fibroblasts exposed to eluates of composite resins used for direct and indirect restorations', EUR J ORAL SCI, Jg. 115, Nr. 5, S. 397-407. https://doi.org/10.1111/j.1600-0722.2007.00478.x

APA

Bakopoulou, A., Tsiftsoglou, A., Galaktidou, G., Markala, D., Triviai, I., & Garefis, P. (2007). Patterns of cell death and cell cycle profiles of cultured WEHI 13 var fibroblasts exposed to eluates of composite resins used for direct and indirect restorations. EUR J ORAL SCI, 115(5), 397-407. https://doi.org/10.1111/j.1600-0722.2007.00478.x

Vancouver

Bakopoulou A, Tsiftsoglou A, Galaktidou G, Markala D, Triviai I, Garefis P. Patterns of cell death and cell cycle profiles of cultured WEHI 13 var fibroblasts exposed to eluates of composite resins used for direct and indirect restorations. EUR J ORAL SCI. 2007 Okt;115(5):397-407. https://doi.org/10.1111/j.1600-0722.2007.00478.x

Bibtex

@article{aee11c2158a9491b8823027c5270b356,
title = "Patterns of cell death and cell cycle profiles of cultured WEHI 13 var fibroblasts exposed to eluates of composite resins used for direct and indirect restorations",
abstract = "Previous studies have shown that in vitro exposure to single compounds released from composite resins may induce cell death. In the present study the effects of eluates from commercially available composite resins used for direct or indirect restorations were evaluated on the cell cycle progression and type of cell death of cultured WEHI 13 var fibroblasts. Cells exposed to eluates of the materials were assessed for cytotoxicity by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for cell death, for cell cycle profiles by flow cytometry, for caspase-3 biochemically and by immunocytochemistry, and for morphological changes by fluorescence microscopy with acridine orange. The direct composite resin eluates induced extensive apoptosis, followed by secondary necrosis. This was accompanied by cell enlargement, micromultinucleation, chromatin disintegration, cell cycle arrest at different phases, and caspase-3 activation. The composites for indirect restorations were much less cytotoxic at all biological end-points investigated. The findings suggest that composite resins used for direct and indirect dental restorations differ in their cytotoxic potential and their ability to affect basic cellular functions. This underlines the impact of improved polymerization with respect to their biologic behavior.",
keywords = "Analysis of Variance, Animals, Caspase 3, Cell Cycle, Cell Death, Cell Membrane, Cell Shape, Cells, Cultured, Composite Resins, Dental Restoration, Permanent, Enzyme Activation, Fibroblasts, Flow Cytometry, Mice, Mice, Inbred BALB C, Microscopy, Fluorescence, Journal Article, Research Support, Non-U.S. Gov't",
author = "Athina Bakopoulou and Asterios Tsiftsoglou and Grammati Galaktidou and Dimitra Markala and Ioanna Triviai and Pavlos Garefis",
year = "2007",
month = oct,
doi = "10.1111/j.1600-0722.2007.00478.x",
language = "English",
volume = "115",
pages = "397--407",
journal = "EUR J ORAL SCI",
issn = "0909-8836",
publisher = "Blackwell Munksgaard",
number = "5",

}

RIS

TY - JOUR

T1 - Patterns of cell death and cell cycle profiles of cultured WEHI 13 var fibroblasts exposed to eluates of composite resins used for direct and indirect restorations

AU - Bakopoulou, Athina

AU - Tsiftsoglou, Asterios

AU - Galaktidou, Grammati

AU - Markala, Dimitra

AU - Triviai, Ioanna

AU - Garefis, Pavlos

PY - 2007/10

Y1 - 2007/10

N2 - Previous studies have shown that in vitro exposure to single compounds released from composite resins may induce cell death. In the present study the effects of eluates from commercially available composite resins used for direct or indirect restorations were evaluated on the cell cycle progression and type of cell death of cultured WEHI 13 var fibroblasts. Cells exposed to eluates of the materials were assessed for cytotoxicity by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for cell death, for cell cycle profiles by flow cytometry, for caspase-3 biochemically and by immunocytochemistry, and for morphological changes by fluorescence microscopy with acridine orange. The direct composite resin eluates induced extensive apoptosis, followed by secondary necrosis. This was accompanied by cell enlargement, micromultinucleation, chromatin disintegration, cell cycle arrest at different phases, and caspase-3 activation. The composites for indirect restorations were much less cytotoxic at all biological end-points investigated. The findings suggest that composite resins used for direct and indirect dental restorations differ in their cytotoxic potential and their ability to affect basic cellular functions. This underlines the impact of improved polymerization with respect to their biologic behavior.

AB - Previous studies have shown that in vitro exposure to single compounds released from composite resins may induce cell death. In the present study the effects of eluates from commercially available composite resins used for direct or indirect restorations were evaluated on the cell cycle progression and type of cell death of cultured WEHI 13 var fibroblasts. Cells exposed to eluates of the materials were assessed for cytotoxicity by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for cell death, for cell cycle profiles by flow cytometry, for caspase-3 biochemically and by immunocytochemistry, and for morphological changes by fluorescence microscopy with acridine orange. The direct composite resin eluates induced extensive apoptosis, followed by secondary necrosis. This was accompanied by cell enlargement, micromultinucleation, chromatin disintegration, cell cycle arrest at different phases, and caspase-3 activation. The composites for indirect restorations were much less cytotoxic at all biological end-points investigated. The findings suggest that composite resins used for direct and indirect dental restorations differ in their cytotoxic potential and their ability to affect basic cellular functions. This underlines the impact of improved polymerization with respect to their biologic behavior.

KW - Analysis of Variance

KW - Animals

KW - Caspase 3

KW - Cell Cycle

KW - Cell Death

KW - Cell Membrane

KW - Cell Shape

KW - Cells, Cultured

KW - Composite Resins

KW - Dental Restoration, Permanent

KW - Enzyme Activation

KW - Fibroblasts

KW - Flow Cytometry

KW - Mice

KW - Mice, Inbred BALB C

KW - Microscopy, Fluorescence

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1111/j.1600-0722.2007.00478.x

DO - 10.1111/j.1600-0722.2007.00478.x

M3 - SCORING: Journal article

C2 - 17850429

VL - 115

SP - 397

EP - 407

JO - EUR J ORAL SCI

JF - EUR J ORAL SCI

SN - 0909-8836

IS - 5

ER -