Patient-based cross-platform comparison of oligonucleotide microarray expression profiles.
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Patient-based cross-platform comparison of oligonucleotide microarray expression profiles. / Schlingemann, Joerg; Habtemichael, Negusse; Ittrich, Carina; Toedt, Grischa; Kramer, Heidi; Hambek, Markus; Knecht, Rainald; Lichter, Peter; Stauber, Roland; Hahn, Meinhard.
in: LAB INVEST, Jahrgang 85, Nr. 8, 8, 2005, S. 1024-1039.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Patient-based cross-platform comparison of oligonucleotide microarray expression profiles.
AU - Schlingemann, Joerg
AU - Habtemichael, Negusse
AU - Ittrich, Carina
AU - Toedt, Grischa
AU - Kramer, Heidi
AU - Hambek, Markus
AU - Knecht, Rainald
AU - Lichter, Peter
AU - Stauber, Roland
AU - Hahn, Meinhard
PY - 2005
Y1 - 2005
N2 - The comparison of gene expression measurements obtained with different technical approaches is of substantial interest in order to clarify whether inter-platform differences may conceal biologically significant information. To address this concern, we analyzed gene expression in a set of head and neck squamous cell carcinoma patients, using both spotted oligonucleotide microarrays made from a large collection of 70-mer probes and commercial arrays produced by in situ synthesis of sets of multiple 25-mer oligonucleotides per gene. Expression measurements were compared for 4425 genes represented on both platforms, which revealed strong correlations between the corresponding data sets. Of note, a global tendency towards smaller absolute ratios was observed when using the 70-mer probes. Real-time quantitative reverse transcription PCR measurements were conducted to verify expression ratios for a subset of genes and achieved good agreement regarding both array platforms. In conclusion, similar profiles of relative gene expression were obtained using arrays of either single 70-mer or multiple short 25-mer oligonucleotide probes per gene. Although qualitative assessments of the expression of individual genes have to be made with caution, our results indicate that the comparison of gene expression profiles generated on these platforms will help to discover disease-related gene signatures in general.
AB - The comparison of gene expression measurements obtained with different technical approaches is of substantial interest in order to clarify whether inter-platform differences may conceal biologically significant information. To address this concern, we analyzed gene expression in a set of head and neck squamous cell carcinoma patients, using both spotted oligonucleotide microarrays made from a large collection of 70-mer probes and commercial arrays produced by in situ synthesis of sets of multiple 25-mer oligonucleotides per gene. Expression measurements were compared for 4425 genes represented on both platforms, which revealed strong correlations between the corresponding data sets. Of note, a global tendency towards smaller absolute ratios was observed when using the 70-mer probes. Real-time quantitative reverse transcription PCR measurements were conducted to verify expression ratios for a subset of genes and achieved good agreement regarding both array platforms. In conclusion, similar profiles of relative gene expression were obtained using arrays of either single 70-mer or multiple short 25-mer oligonucleotide probes per gene. Although qualitative assessments of the expression of individual genes have to be made with caution, our results indicate that the comparison of gene expression profiles generated on these platforms will help to discover disease-related gene signatures in general.
M3 - SCORING: Zeitschriftenaufsatz
VL - 85
SP - 1024
EP - 1039
JO - LAB INVEST
JF - LAB INVEST
SN - 0023-6837
IS - 8
M1 - 8
ER -
