Passive immunization with a monoclonal IgM antibody specific for Strongyloides ratti HSP60 protects mice against challenge infection.
Standard
Passive immunization with a monoclonal IgM antibody specific for Strongyloides ratti HSP60 protects mice against challenge infection. / Nadia, Ben Nouir; Piedavent, Melanie; Osterloh, Anke; Breloer, Minka.
in: VACCINE, Jahrgang 30, Nr. 33, 33, 2012, S. 4971-4976.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Passive immunization with a monoclonal IgM antibody specific for Strongyloides ratti HSP60 protects mice against challenge infection.
AU - Nadia, Ben Nouir
AU - Piedavent, Melanie
AU - Osterloh, Anke
AU - Breloer, Minka
PY - 2012
Y1 - 2012
N2 - It is estimated that 30-100 million people are infected with the pathogenic nematode Strongyloides stercoralis worldwide but parasite control is still based on anti-helminthic treatment. To develop protective vaccination strategies, we use the murine model of Strongyloides ratti infection. We have shown recently that vaccination with alum-precipitated, but not with native or CFA-emulsified S. ratti heat shock protein 60 (srHSP60) conferred protection to challenge infection. Here we describe the generation of a monoclonal IgM specific for srHSP60. Anti-srHSP60 detected human and srHSP60 and stained S. ratti infective larvae in vitro. Passive immunization of mice with monoclonal anti-srHSP60 IgM led to reduced numbers of migrating larvae in lung and head, reduced numbers of parasitic adults in the small intestine and reduced larval output upon S. ratti challenge infection. Taken together, our findings highlight the relevance of srHSP60 as vaccine candidate for the induction of antibody-mediated protection against Strongyloides infection.
AB - It is estimated that 30-100 million people are infected with the pathogenic nematode Strongyloides stercoralis worldwide but parasite control is still based on anti-helminthic treatment. To develop protective vaccination strategies, we use the murine model of Strongyloides ratti infection. We have shown recently that vaccination with alum-precipitated, but not with native or CFA-emulsified S. ratti heat shock protein 60 (srHSP60) conferred protection to challenge infection. Here we describe the generation of a monoclonal IgM specific for srHSP60. Anti-srHSP60 detected human and srHSP60 and stained S. ratti infective larvae in vitro. Passive immunization of mice with monoclonal anti-srHSP60 IgM led to reduced numbers of migrating larvae in lung and head, reduced numbers of parasitic adults in the small intestine and reduced larval output upon S. ratti challenge infection. Taken together, our findings highlight the relevance of srHSP60 as vaccine candidate for the induction of antibody-mediated protection against Strongyloides infection.
KW - Animals
KW - Female
KW - Treatment Outcome
KW - Disease Models, Animal
KW - Mice
KW - Mice, Inbred C57BL
KW - Antibodies, Helminth/administration & dosage/immunology
KW - Antibodies, Monoclonal/administration & dosage/immunology
KW - Antigens, Helminth/immunology
KW - Chaperonin 60/immunology
KW - Feces/parasitology
KW - Immunization, Passive/methods
KW - Immunoglobulin M/administration & dosage/immunology
KW - Intestine, Small/parasitology
KW - Parasite Load
KW - Strongyloides ratti/immunology
KW - Strongyloidiasis/immunology/prevention & control
KW - Animals
KW - Female
KW - Treatment Outcome
KW - Disease Models, Animal
KW - Mice
KW - Mice, Inbred C57BL
KW - Antibodies, Helminth/administration & dosage/immunology
KW - Antibodies, Monoclonal/administration & dosage/immunology
KW - Antigens, Helminth/immunology
KW - Chaperonin 60/immunology
KW - Feces/parasitology
KW - Immunization, Passive/methods
KW - Immunoglobulin M/administration & dosage/immunology
KW - Intestine, Small/parasitology
KW - Parasite Load
KW - Strongyloides ratti/immunology
KW - Strongyloidiasis/immunology/prevention & control
M3 - SCORING: Journal article
VL - 30
SP - 4971
EP - 4976
JO - VACCINE
JF - VACCINE
SN - 0264-410X
IS - 33
M1 - 33
ER -
