PortalPublikationenOsteogenic differentiation of mesenchymal stem cells in fibr...

Osteogenic differentiation of mesenchymal stem cells in fibrin-hydroxyapatite matrix in a 3-dimensional mesh scaffold

Standard

Osteogenic differentiation of mesenchymal stem cells in fibrin-hydroxyapatite matrix in a 3-dimensional mesh scaffold. / Jung, Ole; Hanken, Henning; Smeets, Ralf; Hartjen, Philip; Friedrich, Reinhard E; Schwab, Bettina; Gröbe, Alexander; Heiland, Max; Al-Dam, Ahmed; Eichhorn, Wolfgang; Sehner, Susanne; Kolk, Andreas; Wöltje, Michael; Stein, Jamal M.

in: IN VIVO, Jahrgang 28, Nr. 4, 02.07.2014, S. 477-82.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Jung, O, Hanken, H, Smeets, R, Hartjen, P, Friedrich, RE, Schwab, B, Gröbe, A, Heiland, M, Al-Dam, A, Eichhorn, W, Sehner, S, Kolk, A, Wöltje, M & Stein, JM 2014, 'Osteogenic differentiation of mesenchymal stem cells in fibrin-hydroxyapatite matrix in a 3-dimensional mesh scaffold', IN VIVO, Jg. 28, Nr. 4, S. 477-82.

APA

Jung, O., Hanken, H., Smeets, R., Hartjen, P., Friedrich, R. E., Schwab, B., Gröbe, A., Heiland, M., Al-Dam, A., Eichhorn, W., Sehner, S., Kolk, A., Wöltje, M., & Stein, J. M. (2014). Osteogenic differentiation of mesenchymal stem cells in fibrin-hydroxyapatite matrix in a 3-dimensional mesh scaffold. IN VIVO, 28(4), 477-82.

Vancouver

Jung O, Hanken H, Smeets R, Hartjen P, Friedrich RE, Schwab B et al. Osteogenic differentiation of mesenchymal stem cells in fibrin-hydroxyapatite matrix in a 3-dimensional mesh scaffold. IN VIVO. 2014 Jul 2;28(4):477-82.

Bibtex

@article{4fdfc245011349f3b7c5c6be5a395839,
title = "Osteogenic differentiation of mesenchymal stem cells in fibrin-hydroxyapatite matrix in a 3-dimensional mesh scaffold",
abstract = "AIM: To explore the feasibility of culturing mesenchymal stem cells in an hydroxyapatite-fibrin matrix held by a mesh scaffold and inducing osteogenic differentiation of these cells. The aim was to obtain bone-material in vitro in a desired form.MATERIALS AND METHODS: Rat mesenchymal stem cells were mixed with fibrin and nanocrystalline hydroxyapatite in tubular scaffolds constructed from a poly(L-lactic acid) mesh, and cultured under standard and osteogenic differentiating conditions. Cell viability, cytotoxicity and alkaline phosphatase activity were followed for 3 weeks. Living cells and the expression of bone markers were visualized by fluorescence staining and immunofluorescence staining, respectively. Attachment of cells to the scaffold mesh surface was examined by scanning electron microscopy.RESULTS: Cell viability decreased and cytotoxicity increased rapidly during the first day of culture but stabilized gradually afterwards, indicating fast adaptation of the cells in the matrix-scaffold environment. From day 17, cytotoxicity started to decrease, paralleled by an increase in alkaline phosphatase activity, indicating osteogenic differentiation. A large number of living cells were visible in the matrix and on the mesh scaffold. Expression of collagen type I, osteoponin, osteocalcin and core binding factor 1 were evident under osteogenic differentiation conditions.CONCLUSION: The three-dimensional construction of a fibrin-hydroxyapatite matrix in a biocompatible poly(L-lactic acid) as mesh-scaffold provides a promising carrier for producing bone-material in vitro in a desired form for applications in regenerative medicine.",
keywords = "Alkaline Phosphatase, Animals, Cell Culture Techniques, Cell Differentiation, Cell Survival, Durapatite, Fibrin, Mesenchymal Stromal Cells, Osteogenesis, Rats, Tissue Engineering, Tissue Scaffolds",
author = "Ole Jung and Henning Hanken and Ralf Smeets and Philip Hartjen and Friedrich, {Reinhard E} and Bettina Schwab and Alexander Gr{\"o}be and Max Heiland and Ahmed Al-Dam and Wolfgang Eichhorn and Susanne Sehner and Andreas Kolk and Michael W{\"o}ltje and Stein, {Jamal M}",
note = "Copyright {\textcopyright} 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.",
year = "2014",
month = jul,
day = "2",
language = "English",
volume = "28",
pages = "477--82",
journal = "IN VIVO",
issn = "0258-851X",
publisher = "International Institute of Anticancer Research",
number = "4",

}

RIS

TY - JOUR

T1 - Osteogenic differentiation of mesenchymal stem cells in fibrin-hydroxyapatite matrix in a 3-dimensional mesh scaffold

AU - Jung, Ole

AU - Hanken, Henning

AU - Smeets, Ralf

AU - Hartjen, Philip

AU - Friedrich, Reinhard E

AU - Schwab, Bettina

AU - Gröbe, Alexander

AU - Heiland, Max

AU - Al-Dam, Ahmed

AU - Eichhorn, Wolfgang

AU - Sehner, Susanne

AU - Kolk, Andreas

AU - Wöltje, Michael

AU - Stein, Jamal M

N1 - Copyright © 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

PY - 2014/7/2

Y1 - 2014/7/2

N2 - AIM: To explore the feasibility of culturing mesenchymal stem cells in an hydroxyapatite-fibrin matrix held by a mesh scaffold and inducing osteogenic differentiation of these cells. The aim was to obtain bone-material in vitro in a desired form.MATERIALS AND METHODS: Rat mesenchymal stem cells were mixed with fibrin and nanocrystalline hydroxyapatite in tubular scaffolds constructed from a poly(L-lactic acid) mesh, and cultured under standard and osteogenic differentiating conditions. Cell viability, cytotoxicity and alkaline phosphatase activity were followed for 3 weeks. Living cells and the expression of bone markers were visualized by fluorescence staining and immunofluorescence staining, respectively. Attachment of cells to the scaffold mesh surface was examined by scanning electron microscopy.RESULTS: Cell viability decreased and cytotoxicity increased rapidly during the first day of culture but stabilized gradually afterwards, indicating fast adaptation of the cells in the matrix-scaffold environment. From day 17, cytotoxicity started to decrease, paralleled by an increase in alkaline phosphatase activity, indicating osteogenic differentiation. A large number of living cells were visible in the matrix and on the mesh scaffold. Expression of collagen type I, osteoponin, osteocalcin and core binding factor 1 were evident under osteogenic differentiation conditions.CONCLUSION: The three-dimensional construction of a fibrin-hydroxyapatite matrix in a biocompatible poly(L-lactic acid) as mesh-scaffold provides a promising carrier for producing bone-material in vitro in a desired form for applications in regenerative medicine.

AB - AIM: To explore the feasibility of culturing mesenchymal stem cells in an hydroxyapatite-fibrin matrix held by a mesh scaffold and inducing osteogenic differentiation of these cells. The aim was to obtain bone-material in vitro in a desired form.MATERIALS AND METHODS: Rat mesenchymal stem cells were mixed with fibrin and nanocrystalline hydroxyapatite in tubular scaffolds constructed from a poly(L-lactic acid) mesh, and cultured under standard and osteogenic differentiating conditions. Cell viability, cytotoxicity and alkaline phosphatase activity were followed for 3 weeks. Living cells and the expression of bone markers were visualized by fluorescence staining and immunofluorescence staining, respectively. Attachment of cells to the scaffold mesh surface was examined by scanning electron microscopy.RESULTS: Cell viability decreased and cytotoxicity increased rapidly during the first day of culture but stabilized gradually afterwards, indicating fast adaptation of the cells in the matrix-scaffold environment. From day 17, cytotoxicity started to decrease, paralleled by an increase in alkaline phosphatase activity, indicating osteogenic differentiation. A large number of living cells were visible in the matrix and on the mesh scaffold. Expression of collagen type I, osteoponin, osteocalcin and core binding factor 1 were evident under osteogenic differentiation conditions.CONCLUSION: The three-dimensional construction of a fibrin-hydroxyapatite matrix in a biocompatible poly(L-lactic acid) as mesh-scaffold provides a promising carrier for producing bone-material in vitro in a desired form for applications in regenerative medicine.

KW - Alkaline Phosphatase

KW - Animals

KW - Cell Culture Techniques

KW - Cell Differentiation

KW - Cell Survival

KW - Durapatite

KW - Fibrin

KW - Mesenchymal Stromal Cells

KW - Osteogenesis

KW - Rats

KW - Tissue Engineering

KW - Tissue Scaffolds

M3 - SCORING: Journal article

C2 - 24982212

VL - 28

SP - 477

EP - 482

JO - IN VIVO

JF - IN VIVO

SN - 0258-851X

IS - 4

ER -