Osteoblast response to bioactive glasses in vitro correlates with inorganic phosphate content.
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Osteoblast response to bioactive glasses in vitro correlates with inorganic phosphate content. / Lossdörfer, S; Schwartz, Z; Lohmann, Christoph; Greenspan, D C; Ranly, D M; Boyan, B D.
in: BIOMATERIALS, Jahrgang 25, Nr. 13, 13, 2004, S. 2547-2555.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Osteoblast response to bioactive glasses in vitro correlates with inorganic phosphate content.
AU - Lossdörfer, S
AU - Schwartz, Z
AU - Lohmann, Christoph
AU - Greenspan, D C
AU - Ranly, D M
AU - Boyan, B D
PY - 2004
Y1 - 2004
N2 - Inorganic phosphate (Pi) is a physiological regulator of osteoblasts and chondrocytes, suggesting that phosphate may contribute to the biological response of these cells to bioactive glasses like Bioglass 45S5, which is composed of 45% SiO2, 24.5% CaO, 24.5% Na2O, and 6% P2O5. We investigated the effect of varying the Pi content of bioactive glass disks (0%, 3%, 6% and 12% P2O5) using human osteoblast-like MG63 cells as the model. Cell number on 6% Pi disks was comparable to cultures on tissue culture plastic, but was reduced at higher and lower Pi concentrations. Alkaline phosphatase specific activity of isolated cells and cell layer lysates, as well as PGE2, TGF-beta1 and NO levels in conditioned media, were elevated in cultures grown on bioactive glass and varied with the Pi content. The greatest effects were observed in cultures grown on disks with the lowest Pi concentrations. Thus, growth on the bioactive glasses enhances cell function in comparison with tissue culture plastic and lower Pi content favors osteoblast differentiation.
AB - Inorganic phosphate (Pi) is a physiological regulator of osteoblasts and chondrocytes, suggesting that phosphate may contribute to the biological response of these cells to bioactive glasses like Bioglass 45S5, which is composed of 45% SiO2, 24.5% CaO, 24.5% Na2O, and 6% P2O5. We investigated the effect of varying the Pi content of bioactive glass disks (0%, 3%, 6% and 12% P2O5) using human osteoblast-like MG63 cells as the model. Cell number on 6% Pi disks was comparable to cultures on tissue culture plastic, but was reduced at higher and lower Pi concentrations. Alkaline phosphatase specific activity of isolated cells and cell layer lysates, as well as PGE2, TGF-beta1 and NO levels in conditioned media, were elevated in cultures grown on bioactive glass and varied with the Pi content. The greatest effects were observed in cultures grown on disks with the lowest Pi concentrations. Thus, growth on the bioactive glasses enhances cell function in comparison with tissue culture plastic and lower Pi content favors osteoblast differentiation.
M3 - SCORING: Zeitschriftenaufsatz
VL - 25
SP - 2547
EP - 2555
JO - BIOMATERIALS
JF - BIOMATERIALS
SN - 0142-9612
IS - 13
M1 - 13
ER -
