Origin, maturation, and astroglial transformation of secondary radial glial cells in the developing dentate gyrus.
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Origin, maturation, and astroglial transformation of secondary radial glial cells in the developing dentate gyrus. / Brunne, Bianka; Zhao, Shanting; Derouiche, Amin; Herz, Joachim; May, Petra; Frotscher, Michael; Bock, Hans H.
in: GLIA, Jahrgang 58, Nr. 13, 13, 2010, S. 1553-1569.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Origin, maturation, and astroglial transformation of secondary radial glial cells in the developing dentate gyrus.
AU - Brunne, Bianka
AU - Zhao, Shanting
AU - Derouiche, Amin
AU - Herz, Joachim
AU - May, Petra
AU - Frotscher, Michael
AU - Bock, Hans H
PY - 2010
Y1 - 2010
N2 - The dentate gyrus is a brain region where neurons are continuously born throughout life. In the adult, the role of its radial glia in neurogenesis has attracted much attention over the past years; however, little is known about the generation and differentiation of glial cells and their relationship to radial glia during the ontogenetic development of this brain structure. Here, we combine immunohistochemical phenotyping using antibodies against glial marker proteins with BrdU birthdating to characterize the development of the secondary radial glial scaffold in the dentate gyrus and its potential to differentiate into astrocytes. We demonstrate that the expression of brain lipid-binding protein, GLAST, and glial fibrillary acidic protein (GFAP) characterizes immature differentiating cells confined to an astrocytic fate in the early postnatal dentate gyrus. On the basis of our studies, we propose a model where immature astrocytes migrate radially through the granule cell layer to adopt their final positions in the molecular layer of the dentate gyrus. Time-lapse imaging of acute hippocampal slices from hGFAP-eGFP transgenic mice provides direct evidence for such a migration mode of differentiating astroglial cells in the developing dentate gyrus.
AB - The dentate gyrus is a brain region where neurons are continuously born throughout life. In the adult, the role of its radial glia in neurogenesis has attracted much attention over the past years; however, little is known about the generation and differentiation of glial cells and their relationship to radial glia during the ontogenetic development of this brain structure. Here, we combine immunohistochemical phenotyping using antibodies against glial marker proteins with BrdU birthdating to characterize the development of the secondary radial glial scaffold in the dentate gyrus and its potential to differentiate into astrocytes. We demonstrate that the expression of brain lipid-binding protein, GLAST, and glial fibrillary acidic protein (GFAP) characterizes immature differentiating cells confined to an astrocytic fate in the early postnatal dentate gyrus. On the basis of our studies, we propose a model where immature astrocytes migrate radially through the granule cell layer to adopt their final positions in the molecular layer of the dentate gyrus. Time-lapse imaging of acute hippocampal slices from hGFAP-eGFP transgenic mice provides direct evidence for such a migration mode of differentiating astroglial cells in the developing dentate gyrus.
KW - Animals
KW - Age Factors
KW - Time Factors
KW - Mice
KW - Models, Biological
KW - Green Fluorescent Proteins genetics
KW - Mice, Transgenic
KW - Cell Differentiation physiology
KW - Cell Proliferation
KW - Neuroglia physiology
KW - Animals, Newborn
KW - Analysis of Variance
KW - Embryo, Mammalian
KW - Nerve Tissue Proteins genetics
KW - Bromodeoxyuridine metabolism
KW - Dentate Gyrus cytology
KW - Excitatory Amino Acid Transporter 1 metabolism
KW - Gene Expression Regulation, Developmental physiology
KW - Glial Fibrillary Acidic Protein genetics
KW - Animals
KW - Age Factors
KW - Time Factors
KW - Mice
KW - Models, Biological
KW - Green Fluorescent Proteins genetics
KW - Mice, Transgenic
KW - Cell Differentiation physiology
KW - Cell Proliferation
KW - Neuroglia physiology
KW - Animals, Newborn
KW - Analysis of Variance
KW - Embryo, Mammalian
KW - Nerve Tissue Proteins genetics
KW - Bromodeoxyuridine metabolism
KW - Dentate Gyrus cytology
KW - Excitatory Amino Acid Transporter 1 metabolism
KW - Gene Expression Regulation, Developmental physiology
KW - Glial Fibrillary Acidic Protein genetics
M3 - SCORING: Zeitschriftenaufsatz
VL - 58
SP - 1553
EP - 1569
JO - GLIA
JF - GLIA
SN - 0894-1491
IS - 13
M1 - 13
ER -
