Oriented, multimeric biointerfaces of the l1 cell adhesion molecule: an approach to enhance neuronal and neural stem cell functions on 2-d and 3-d polymer substrates.

Standard

Oriented, multimeric biointerfaces of the l1 cell adhesion molecule: an approach to enhance neuronal and neural stem cell functions on 2-d and 3-d polymer substrates. / Cherry, Jocie F; Carlson, Aaron L; Benarba, Farah L; Sommerfeld, Sven D; Verma, Devendra; Loers, Gabriele; Kohn, Joachim; Schachner, Melitta; Moghe, Prabhas V.

in: BIOINTERPHASES, Jahrgang 7, Nr. 1-4, 1-4, 2012, S. 22.

Publikationen: SCORING: Beitrag in Fachzeitschrift/ZeitungSCORING: ZeitschriftenaufsatzForschungBegutachtung

Harvard

APA

Vancouver

Bibtex

@article{b2f98dbc5a224102aa9ec47c116d2ab7,
title = "Oriented, multimeric biointerfaces of the l1 cell adhesion molecule: an approach to enhance neuronal and neural stem cell functions on 2-d and 3-d polymer substrates.",
abstract = "This article focuses on elucidating the key presentation features of neurotrophic ligands at polymer interfaces. Different biointerfacial configurations of the human neural cell adhesion molecule L1 were established on two-dimensional films and three-dimensional fibrous scaffolds of synthetic tyrosine-derived polycarbonate polymers and probed for surface concentrations, microscale organization, and effects on cultured primary neurons and neural stem cells. Underlying polymer substrates were modified with varying combinations of protein A and poly-D: -lysine to modulate the immobilization and presentation of the Fc fusion fragment of the extracellular domain of L1 (L1-Fc). When presented as an oriented and multimeric configuration from protein A-pretreated polymers, L1-Fc significantly increased neurite outgrowth of rodent spinal cord neurons and cerebellar neurons as early as 24 h compared to the traditional presentation via adsorption onto surfaces treated with poly-D: -lysine. Cultures of human neural progenitor cells screened on the L1-Fc/polymer biointerfaces showed significantly enhanced neuronal differentiation and neuritogenesis on all protein A oriented substrates. Notably, the highest degree of ?III-tubulin expression for cells in 3-D fibrous scaffolds were observed in protein A oriented substrates with PDL pretreatment, suggesting combined effects of cell attachment to polycationic charged substrates with subcellular topography along with L1-mediated adhesion mediating neuronal differentiation. Together, these findings highlight the promise of displays of multimeric neural adhesion ligands via biointerfacially engineered substrates to {"}cooperatively{"} enhance neuronal phenotypes on polymers of relevance to tissue engineering.",
author = "Cherry, {Jocie F} and Carlson, {Aaron L} and Benarba, {Farah L} and Sommerfeld, {Sven D} and Devendra Verma and Gabriele Loers and Joachim Kohn and Melitta Schachner and Moghe, {Prabhas V}",
year = "2012",
language = "English",
volume = "7",
pages = "22",
journal = "BIOINTERPHASES",
issn = "1934-8630",
publisher = "American Vacuum Society",
number = "1-4",

}

RIS

TY - JOUR

T1 - Oriented, multimeric biointerfaces of the l1 cell adhesion molecule: an approach to enhance neuronal and neural stem cell functions on 2-d and 3-d polymer substrates.

AU - Cherry, Jocie F

AU - Carlson, Aaron L

AU - Benarba, Farah L

AU - Sommerfeld, Sven D

AU - Verma, Devendra

AU - Loers, Gabriele

AU - Kohn, Joachim

AU - Schachner, Melitta

AU - Moghe, Prabhas V

PY - 2012

Y1 - 2012

N2 - This article focuses on elucidating the key presentation features of neurotrophic ligands at polymer interfaces. Different biointerfacial configurations of the human neural cell adhesion molecule L1 were established on two-dimensional films and three-dimensional fibrous scaffolds of synthetic tyrosine-derived polycarbonate polymers and probed for surface concentrations, microscale organization, and effects on cultured primary neurons and neural stem cells. Underlying polymer substrates were modified with varying combinations of protein A and poly-D: -lysine to modulate the immobilization and presentation of the Fc fusion fragment of the extracellular domain of L1 (L1-Fc). When presented as an oriented and multimeric configuration from protein A-pretreated polymers, L1-Fc significantly increased neurite outgrowth of rodent spinal cord neurons and cerebellar neurons as early as 24 h compared to the traditional presentation via adsorption onto surfaces treated with poly-D: -lysine. Cultures of human neural progenitor cells screened on the L1-Fc/polymer biointerfaces showed significantly enhanced neuronal differentiation and neuritogenesis on all protein A oriented substrates. Notably, the highest degree of ?III-tubulin expression for cells in 3-D fibrous scaffolds were observed in protein A oriented substrates with PDL pretreatment, suggesting combined effects of cell attachment to polycationic charged substrates with subcellular topography along with L1-mediated adhesion mediating neuronal differentiation. Together, these findings highlight the promise of displays of multimeric neural adhesion ligands via biointerfacially engineered substrates to "cooperatively" enhance neuronal phenotypes on polymers of relevance to tissue engineering.

AB - This article focuses on elucidating the key presentation features of neurotrophic ligands at polymer interfaces. Different biointerfacial configurations of the human neural cell adhesion molecule L1 were established on two-dimensional films and three-dimensional fibrous scaffolds of synthetic tyrosine-derived polycarbonate polymers and probed for surface concentrations, microscale organization, and effects on cultured primary neurons and neural stem cells. Underlying polymer substrates were modified with varying combinations of protein A and poly-D: -lysine to modulate the immobilization and presentation of the Fc fusion fragment of the extracellular domain of L1 (L1-Fc). When presented as an oriented and multimeric configuration from protein A-pretreated polymers, L1-Fc significantly increased neurite outgrowth of rodent spinal cord neurons and cerebellar neurons as early as 24 h compared to the traditional presentation via adsorption onto surfaces treated with poly-D: -lysine. Cultures of human neural progenitor cells screened on the L1-Fc/polymer biointerfaces showed significantly enhanced neuronal differentiation and neuritogenesis on all protein A oriented substrates. Notably, the highest degree of ?III-tubulin expression for cells in 3-D fibrous scaffolds were observed in protein A oriented substrates with PDL pretreatment, suggesting combined effects of cell attachment to polycationic charged substrates with subcellular topography along with L1-mediated adhesion mediating neuronal differentiation. Together, these findings highlight the promise of displays of multimeric neural adhesion ligands via biointerfacially engineered substrates to "cooperatively" enhance neuronal phenotypes on polymers of relevance to tissue engineering.

M3 - SCORING: Journal article

VL - 7

SP - 22

JO - BIOINTERPHASES

JF - BIOINTERPHASES

SN - 1934-8630

IS - 1-4

M1 - 1-4

ER -