Organelle segregation into Plasmodium liver stage merozoites.
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Organelle segregation into Plasmodium liver stage merozoites. / Stanway, Rebecca R; Mueller, Nancy; Zobiak, Bernd; Graewe, Stefanie; Froehlke, Ulrike; Zessin, Patrick J M; Aepfelbacher, Martin; Heussler, Volker T.
in: CELL MICROBIOL, Jahrgang 13, Nr. 11, 11, 2011, S. 1768-1782.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Organelle segregation into Plasmodium liver stage merozoites.
AU - Stanway, Rebecca R
AU - Mueller, Nancy
AU - Zobiak, Bernd
AU - Graewe, Stefanie
AU - Froehlke, Ulrike
AU - Zessin, Patrick J M
AU - Aepfelbacher, Martin
AU - Heussler, Volker T
PY - 2011
Y1 - 2011
N2 - The liver stage of the Plasmodium parasite remains one of the most promising targets for intervention against malaria as it is clinically silent, precedes the symptomatic blood stage and represents a bottleneck in the parasite life cycle. However, many aspects of the development of the parasite during this stage are far from understood. During the liver stage, the parasite undergoes extensive replication, forming tens of thousands of infectious merozoites from each invading sporozoite. This implies a very efficient and accurate process of cytokinesis and thus also of organelle development and segregation. We have generated for the first time Plasmodium berghei double-fluorescent parasite lines, allowing visualization of the apicoplast, mitochondria and nuclei in live liver stage parasites. Using these we have seen that in parallel with nuclear division, the apicoplast and mitochondrion become two extensively branched and intertwining structures. The organelles then undergo impressive morphological and positional changes prior to cell division. To form merozoites, the parasite undergoes cytokinesis and the complex process of organelle development and segregation into the forming daughter merozoites could be analysed in detail using the newly generated transgenic parasites.
AB - The liver stage of the Plasmodium parasite remains one of the most promising targets for intervention against malaria as it is clinically silent, precedes the symptomatic blood stage and represents a bottleneck in the parasite life cycle. However, many aspects of the development of the parasite during this stage are far from understood. During the liver stage, the parasite undergoes extensive replication, forming tens of thousands of infectious merozoites from each invading sporozoite. This implies a very efficient and accurate process of cytokinesis and thus also of organelle development and segregation. We have generated for the first time Plasmodium berghei double-fluorescent parasite lines, allowing visualization of the apicoplast, mitochondria and nuclei in live liver stage parasites. Using these we have seen that in parallel with nuclear division, the apicoplast and mitochondrion become two extensively branched and intertwining structures. The organelles then undergo impressive morphological and positional changes prior to cell division. To form merozoites, the parasite undergoes cytokinesis and the complex process of organelle development and segregation into the forming daughter merozoites could be analysed in detail using the newly generated transgenic parasites.
KW - Microscopy, Fluorescence
KW - Genes, Reporter
KW - Cytokinesis
KW - Liver/parasitology
KW - Luminescent Proteins/genetics/metabolism
KW - Merozoites/growth & development/physiology
KW - Organelles/physiology/ultrastructure
KW - Plasmodium berghei/growth & development/physiology
KW - Staining and Labeling/methods
KW - Microscopy, Fluorescence
KW - Genes, Reporter
KW - Cytokinesis
KW - Liver/parasitology
KW - Luminescent Proteins/genetics/metabolism
KW - Merozoites/growth & development/physiology
KW - Organelles/physiology/ultrastructure
KW - Plasmodium berghei/growth & development/physiology
KW - Staining and Labeling/methods
M3 - SCORING: Journal article
VL - 13
SP - 1768
EP - 1782
JO - CELL MICROBIOL
JF - CELL MICROBIOL
SN - 1462-5814
IS - 11
M1 - 11
ER -
