Optimizing E. coli-based membrane protein production using Lemo21(DE3) and GFP-fusions
Standard
Optimizing E. coli-based membrane protein production using Lemo21(DE3) and GFP-fusions. / Hjelm, Anna; Schlegel, Susan; Baumgarten, Thomas; Klepsch, Mirjam; Wickström, David; Drew, David; de Gier, Jan-Willem.
in: Methods Mol Biol, Jahrgang 1033, 2013, S. 381-400.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Optimizing E. coli-based membrane protein production using Lemo21(DE3) and GFP-fusions
AU - Hjelm, Anna
AU - Schlegel, Susan
AU - Baumgarten, Thomas
AU - Klepsch, Mirjam
AU - Wickström, David
AU - Drew, David
AU - de Gier, Jan-Willem
PY - 2013
Y1 - 2013
N2 - Optimizing the conditions for the overexpression of membrane proteins in E. coli and their subsequent purification is usually a laborious and time-consuming process. Combining the Lemo21(DE3) strain, which conveniently allows to identify the optimal expression intensity of a membrane protein using only one strain, and membrane proteins C-terminally fused to Green Fluorescent Protein (GFP) greatly facilitates the production of high-quality membrane protein material for functional and structural studies.
AB - Optimizing the conditions for the overexpression of membrane proteins in E. coli and their subsequent purification is usually a laborious and time-consuming process. Combining the Lemo21(DE3) strain, which conveniently allows to identify the optimal expression intensity of a membrane protein using only one strain, and membrane proteins C-terminally fused to Green Fluorescent Protein (GFP) greatly facilitates the production of high-quality membrane protein material for functional and structural studies.
KW - Bioreactors
KW - Escherichia coli
KW - Fermentation
KW - Gene Expression Regulation, Bacterial
KW - Genetic Vectors
KW - Green Fluorescent Proteins
KW - Membrane Proteins
KW - Recombinant Fusion Proteins
KW - Transformation, Bacterial
KW - Journal Article
KW - Research Support, N.I.H., Extramural
KW - Research Support, Non-U.S. Gov't
U2 - 10.1007/978-1-62703-487-6_24
DO - 10.1007/978-1-62703-487-6_24
M3 - SCORING: Journal article
C2 - 23996190
VL - 1033
SP - 381
EP - 400
JO - Methods Mol Biol
JF - Methods Mol Biol
SN - 1064-3745
ER -