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Open-source genomic analysis of Shiga-toxin-producing E. coli O104:H4.

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Open-source genomic analysis of Shiga-toxin-producing E. coli O104:H4. / Rohde, Holger; Qin, Junjie; Cui, Yujun; Li, Dongfang; Loman, Nicholas J; Hentschke, Moritz; Chen, Wentong; Pu, Fei; Peng, Yangqing; Li, Junhua; Xi, Feng; Li, Shenghui; Li, Yin; Zhang, Zhaoxi; Yang, Xianwei; Zhao, Meiru; Wang, Peng; Guan, Yuanlin; Cen, Zhong; Zhao, Xiangna; Christner, Martin; Kobbe, Robin; Loos, Sebastian; Oh, Jun; Yang, Liang; Danchin, Antoine; Gao, George F; Song, Yajun; Li, Yingrui; Yang, Huanming; Wang, Jian; Xu, Jianguo; Pallen, Mark J; Wang, Jun; Aepfelbacher, Martin; Yang, Ruifu; Consortium, E. Coli O104:H4 Genome Analysis Crowd-Sourcing.

in: NEW ENGL J MED, Jahrgang 365, Nr. 8, 8, 2011, S. 718-724.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Rohde, H, Qin, J, Cui, Y, Li, D, Loman, NJ, Hentschke, M, Chen, W, Pu, F, Peng, Y, Li, J, Xi, F, Li, S, Li, Y, Zhang, Z, Yang, X, Zhao, M, Wang, P, Guan, Y, Cen, Z, Zhao, X, Christner, M, Kobbe, R, Loos, S, Oh, J, Yang, L, Danchin, A, Gao, GF, Song, Y, Li, Y, Yang, H, Wang, J, Xu, J, Pallen, MJ, Wang, J, Aepfelbacher, M, Yang, R & Consortium, ECOHGAC-S 2011, 'Open-source genomic analysis of Shiga-toxin-producing E. coli O104:H4.', NEW ENGL J MED, Jg. 365, Nr. 8, 8, S. 718-724. <http://www.ncbi.nlm.nih.gov/pubmed/21793736?dopt=Citation>

APA

Rohde, H., Qin, J., Cui, Y., Li, D., Loman, N. J., Hentschke, M., Chen, W., Pu, F., Peng, Y., Li, J., Xi, F., Li, S., Li, Y., Zhang, Z., Yang, X., Zhao, M., Wang, P., Guan, Y., Cen, Z., ... Consortium, E. C. OH. G. A. C-S. (2011). Open-source genomic analysis of Shiga-toxin-producing E. coli O104:H4. NEW ENGL J MED, 365(8), 718-724. [8]. http://www.ncbi.nlm.nih.gov/pubmed/21793736?dopt=Citation

Vancouver

Rohde H, Qin J, Cui Y, Li D, Loman NJ, Hentschke M et al. Open-source genomic analysis of Shiga-toxin-producing E. coli O104:H4. NEW ENGL J MED. 2011;365(8):718-724. 8.

Bibtex

@article{e1d9dc41e81c459cb1ba2e21e2d466db,
title = "Open-source genomic analysis of Shiga-toxin-producing E. coli O104:H4.",
abstract = "An outbreak caused by Shiga-toxin–producing Escherichia coli O104:H4 occurred in Germany in May and June of 2011, with more than 3000 persons infected. Here, we report a cluster of cases associated with a single family and describe an open-source genomic analysis of an isolate from one member of the family. This analysis involved the use of rapid, bench-top DNA sequencing technology, open-source data release, and prompt crowd-sourced analyses. In less than a week, these studies revealed that the outbreak strain belonged to an enteroaggregative E. coli lineage that had acquired genes for Shiga toxin 2 and for antibiotic resistance.",
keywords = "Germany, Humans, Male, Female, Adolescent, Child, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Polymerase Chain Reaction, Feces/microbiology, Bacterial Typing Techniques, Diarrhea/epidemiology/microbiology, Escherichia coli Infections/*microbiology, *Genome, Bacterial, Hemolytic-Uremic Syndrome/epidemiology/*microbiology, Plasmids/genetics, Shiga-Toxigenic Escherichia coli/classification/*genetics/isolation & purification, Germany, Humans, Male, Female, Adolescent, Child, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Polymerase Chain Reaction, Feces/microbiology, Bacterial Typing Techniques, Diarrhea/epidemiology/microbiology, Escherichia coli Infections/*microbiology, *Genome, Bacterial, Hemolytic-Uremic Syndrome/epidemiology/*microbiology, Plasmids/genetics, Shiga-Toxigenic Escherichia coli/classification/*genetics/isolation & purification",
author = "Holger Rohde and Junjie Qin and Yujun Cui and Dongfang Li and Loman, {Nicholas J} and Moritz Hentschke and Wentong Chen and Fei Pu and Yangqing Peng and Junhua Li and Feng Xi and Shenghui Li and Yin Li and Zhaoxi Zhang and Xianwei Yang and Meiru Zhao and Peng Wang and Yuanlin Guan and Zhong Cen and Xiangna Zhao and Martin Christner and Robin Kobbe and Sebastian Loos and Jun Oh and Liang Yang and Antoine Danchin and Gao, {George F} and Yajun Song and Yingrui Li and Huanming Yang and Jian Wang and Jianguo Xu and Pallen, {Mark J} and Jun Wang and Martin Aepfelbacher and Ruifu Yang and Consortium, {E. Coli O104:H4 Genome Analysis Crowd-Sourcing}",
year = "2011",
language = "English",
volume = "365",
pages = "718--724",
journal = "NEW ENGL J MED",
issn = "0028-4793",
publisher = "Massachussetts Medical Society",
number = "8",

}

RIS

TY - JOUR

T1 - Open-source genomic analysis of Shiga-toxin-producing E. coli O104:H4.

AU - Rohde, Holger

AU - Qin, Junjie

AU - Cui, Yujun

AU - Li, Dongfang

AU - Loman, Nicholas J

AU - Hentschke, Moritz

AU - Chen, Wentong

AU - Pu, Fei

AU - Peng, Yangqing

AU - Li, Junhua

AU - Xi, Feng

AU - Li, Shenghui

AU - Li, Yin

AU - Zhang, Zhaoxi

AU - Yang, Xianwei

AU - Zhao, Meiru

AU - Wang, Peng

AU - Guan, Yuanlin

AU - Cen, Zhong

AU - Zhao, Xiangna

AU - Christner, Martin

AU - Kobbe, Robin

AU - Loos, Sebastian

AU - Oh, Jun

AU - Yang, Liang

AU - Danchin, Antoine

AU - Gao, George F

AU - Song, Yajun

AU - Li, Yingrui

AU - Yang, Huanming

AU - Wang, Jian

AU - Xu, Jianguo

AU - Pallen, Mark J

AU - Wang, Jun

AU - Aepfelbacher, Martin

AU - Yang, Ruifu

AU - Consortium, E. Coli O104:H4 Genome Analysis Crowd-Sourcing

PY - 2011

Y1 - 2011

N2 - An outbreak caused by Shiga-toxin–producing Escherichia coli O104:H4 occurred in Germany in May and June of 2011, with more than 3000 persons infected. Here, we report a cluster of cases associated with a single family and describe an open-source genomic analysis of an isolate from one member of the family. This analysis involved the use of rapid, bench-top DNA sequencing technology, open-source data release, and prompt crowd-sourced analyses. In less than a week, these studies revealed that the outbreak strain belonged to an enteroaggregative E. coli lineage that had acquired genes for Shiga toxin 2 and for antibiotic resistance.

AB - An outbreak caused by Shiga-toxin–producing Escherichia coli O104:H4 occurred in Germany in May and June of 2011, with more than 3000 persons infected. Here, we report a cluster of cases associated with a single family and describe an open-source genomic analysis of an isolate from one member of the family. This analysis involved the use of rapid, bench-top DNA sequencing technology, open-source data release, and prompt crowd-sourced analyses. In less than a week, these studies revealed that the outbreak strain belonged to an enteroaggregative E. coli lineage that had acquired genes for Shiga toxin 2 and for antibiotic resistance.

KW - Germany

KW - Humans

KW - Male

KW - Female

KW - Adolescent

KW - Child

KW - Molecular Sequence Data

KW - Phylogeny

KW - Sequence Analysis, DNA

KW - Polymerase Chain Reaction

KW - Feces/microbiology

KW - Bacterial Typing Techniques

KW - Diarrhea/epidemiology/microbiology

KW - Escherichia coli Infections/microbiology

KW - Genome, Bacterial

KW - Hemolytic-Uremic Syndrome/epidemiology/microbiology

KW - Plasmids/genetics

KW - Shiga-Toxigenic Escherichia coli/classification/genetics/isolation & purification

KW - Germany

KW - Humans

KW - Male

KW - Female

KW - Adolescent

KW - Child

KW - Molecular Sequence Data

KW - Phylogeny

KW - Sequence Analysis, DNA

KW - Polymerase Chain Reaction

KW - Feces/microbiology

KW - Bacterial Typing Techniques

KW - Diarrhea/epidemiology/microbiology

KW - Escherichia coli Infections/microbiology

KW - Genome, Bacterial

KW - Hemolytic-Uremic Syndrome/epidemiology/microbiology

KW - Plasmids/genetics

KW - Shiga-Toxigenic Escherichia coli/classification/genetics/isolation & purification

M3 - SCORING: Journal article

VL - 365

SP - 718

EP - 724

JO - NEW ENGL J MED

JF - NEW ENGL J MED

SN - 0028-4793

IS - 8

M1 - 8

ER -