Obesity and NK cells affect the expression of the long form of the leptin receptor Ob-Rb in liver of F344 rats.
Standard
Obesity and NK cells affect the expression of the long form of the leptin receptor Ob-Rb in liver of F344 rats. / Wrann, Christiane D; Ehmer, Ursula; Lautenbach, Anne; Kuhlmann, Susanne; Nave, Heike.
in: EXP TOXICOL PATHOL, Jahrgang 62, Nr. 1, 1, 2010, S. 1-8.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Obesity and NK cells affect the expression of the long form of the leptin receptor Ob-Rb in liver of F344 rats.
AU - Wrann, Christiane D
AU - Ehmer, Ursula
AU - Lautenbach, Anne
AU - Kuhlmann, Susanne
AU - Nave, Heike
PY - 2010
Y1 - 2010
N2 - In obesity, the regulatory effects of leptin, a primarily adipocyte-derived hormone, are severely disturbed affecting the control of energy homeostasis and immune functions. In addition, recent studies indicate that specific immune cells can affect glucose and lipid metabolism of liver. However, the contribution of body weight and immune cells, such as Natural Killer (NK) cells, to the regulation of the leptin-receptor expression remains elusive. Therefore, we investigated the expression of the signal-transducing long form of the leptin receptor (Ob-Rb) in diet-induced obesity and after adoptive cross-over NK cell transfer between normal weight and obese male F344 rats. Expression of Ob-Rb was significantly increased in liver in diet-induced obese rats as compared to normal weight littermates. Similarly, the expression of Ob-Rb was higher in liver of obese animals that received NK cells from either obese or normal weight donors as compared to normal weight animals that received NK cells from normal weight donors. Interestingly, normal weight animals that were transferred with NK cells from obese donors also showed a tendency towards a higher Ob-Rb expression. In contrast to the findings in liver, the expression of Ob-Rb in spleen or lung remained unaffected by changes in body weight or cross-over NK cell transfer. Our results suggest that the expression of Ob-Rb mRNA in liver, but not in spleen or lung, is dependent on the body weight but can also be influenced by NK cells, thereby indicating a bidirectional cross-talk between the metabolic and the immune system.
AB - In obesity, the regulatory effects of leptin, a primarily adipocyte-derived hormone, are severely disturbed affecting the control of energy homeostasis and immune functions. In addition, recent studies indicate that specific immune cells can affect glucose and lipid metabolism of liver. However, the contribution of body weight and immune cells, such as Natural Killer (NK) cells, to the regulation of the leptin-receptor expression remains elusive. Therefore, we investigated the expression of the signal-transducing long form of the leptin receptor (Ob-Rb) in diet-induced obesity and after adoptive cross-over NK cell transfer between normal weight and obese male F344 rats. Expression of Ob-Rb was significantly increased in liver in diet-induced obese rats as compared to normal weight littermates. Similarly, the expression of Ob-Rb was higher in liver of obese animals that received NK cells from either obese or normal weight donors as compared to normal weight animals that received NK cells from normal weight donors. Interestingly, normal weight animals that were transferred with NK cells from obese donors also showed a tendency towards a higher Ob-Rb expression. In contrast to the findings in liver, the expression of Ob-Rb in spleen or lung remained unaffected by changes in body weight or cross-over NK cell transfer. Our results suggest that the expression of Ob-Rb mRNA in liver, but not in spleen or lung, is dependent on the body weight but can also be influenced by NK cells, thereby indicating a bidirectional cross-talk between the metabolic and the immune system.
KW - Animals
KW - Male
KW - Rats
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Flow Cytometry
KW - Gene Expression Regulation/physiology
KW - Signal Transduction/physiology
KW - RNA, Messenger/genetics
KW - Adoptive Transfer
KW - Rats, Inbred F344
KW - Body Weight/physiology
KW - Killer Cells, Natural/physiology
KW - Liver/physiology
KW - Lung/physiology
KW - Obesity/physiopathology
KW - Receptors, Leptin/biosynthesis/genetics/physiology
KW - Spleen/physiology
KW - Animals
KW - Male
KW - Rats
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Flow Cytometry
KW - Gene Expression Regulation/physiology
KW - Signal Transduction/physiology
KW - RNA, Messenger/genetics
KW - Adoptive Transfer
KW - Rats, Inbred F344
KW - Body Weight/physiology
KW - Killer Cells, Natural/physiology
KW - Liver/physiology
KW - Lung/physiology
KW - Obesity/physiopathology
KW - Receptors, Leptin/biosynthesis/genetics/physiology
KW - Spleen/physiology
M3 - SCORING: Journal article
VL - 62
SP - 1
EP - 8
IS - 1
M1 - 1
ER -