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Nuclear factor of activated T cells regulates the expression of interleukin-4 in Th2 cells in an all-or-none fashion

Standard

Nuclear factor of activated T cells regulates the expression of interleukin-4 in Th2 cells in an all-or-none fashion. / Köck, Juliana; Kreher, Stephan; Lehmann, Katrin; Riedel, René; Bardua, Markus; Lischke, Timo; Jargosch, Manja; Haftmann, Claudia; Bendfeldt, Hanna; Hatam, Farahnaz; Mashreghi, Mir-Farzin; Baumgrass, Ria; Radbruch, Andreas; Chang, Hyun-Dong.

in: J BIOL CHEM, Jahrgang 289, Nr. 39, 26.09.2014, S. 26752-26761.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Köck, J, Kreher, S, Lehmann, K, Riedel, R, Bardua, M, Lischke, T, Jargosch, M, Haftmann, C, Bendfeldt, H, Hatam, F, Mashreghi, M-F, Baumgrass, R, Radbruch, A & Chang, H-D 2014, 'Nuclear factor of activated T cells regulates the expression of interleukin-4 in Th2 cells in an all-or-none fashion', J BIOL CHEM, Jg. 289, Nr. 39, S. 26752-26761. https://doi.org/10.1074/jbc.M114.587865

APA

Köck, J., Kreher, S., Lehmann, K., Riedel, R., Bardua, M., Lischke, T., Jargosch, M., Haftmann, C., Bendfeldt, H., Hatam, F., Mashreghi, M-F., Baumgrass, R., Radbruch, A., & Chang, H-D. (2014). Nuclear factor of activated T cells regulates the expression of interleukin-4 in Th2 cells in an all-or-none fashion. J BIOL CHEM, 289(39), 26752-26761. https://doi.org/10.1074/jbc.M114.587865

Vancouver

Köck J, Kreher S, Lehmann K, Riedel R, Bardua M, Lischke T et al. Nuclear factor of activated T cells regulates the expression of interleukin-4 in Th2 cells in an all-or-none fashion. J BIOL CHEM. 2014 Sep 26;289(39):26752-26761. https://doi.org/10.1074/jbc.M114.587865

Bibtex

@article{0c15342145a8491780f9c39ee99bda59,
title = "Nuclear factor of activated T cells regulates the expression of interleukin-4 in Th2 cells in an all-or-none fashion",
abstract = "Th2 memory lymphocytes have imprinted their Il4 genes epigenetically for expression in dependence of T cell receptor restimulation. However, in a given restimulation, not all Th cells with a memory for IL-4 expression express IL-4. Here, we show that in reactivated Th2 cells, the transcription factors NFATc2, NF-kB p65, c-Maf, p300, Brg1, STAT6, and GATA-3 assemble at the Il4 promoter in Th2 cells expressing IL-4 but not in Th2 cells not expressing it. NFATc2 is critical for assembly of this transcription factor complex. Because NFATc2 translocation into the nucleus occurs in an all-or-none fashion, dependent on complete dephosphorylation by calcineurin, NFATc2 controls the frequencies of cells reexpressing Il4, translates analog differences in T cell receptor stimulation into a digital decision for Il4 reexpression, and instructs all reexpressing cells to express the same amount of IL-4. This analog-to-digital conversion may be critical for the immune system to respond to low concentrations of antigens. ",
keywords = "Active Transport, Cell Nucleus/physiology, Adaptor Proteins, Signal Transducing/genetics, Animals, Cell Nucleus/genetics, DNA Helicases/genetics, E1A-Associated p300 Protein/genetics, GATA3 Transcription Factor/genetics, Gene Expression Regulation/physiology, Interleukin-4/biosynthesis, Mice, Mice, Inbred BALB C, Mice, Transgenic, NFATC Transcription Factors/genetics, Nuclear Proteins/genetics, Phosphorylation/physiology, Receptors, Antigen, T-Cell/genetics, Response Elements/physiology, STAT6 Transcription Factor/genetics, Th2 Cells/cytology, Transcription Factor RelA/genetics, Transcription Factors/genetics",
author = "Juliana K{\"o}ck and Stephan Kreher and Katrin Lehmann and Ren{\'e} Riedel and Markus Bardua and Timo Lischke and Manja Jargosch and Claudia Haftmann and Hanna Bendfeldt and Farahnaz Hatam and Mir-Farzin Mashreghi and Ria Baumgrass and Andreas Radbruch and Hyun-Dong Chang",
note = "{\textcopyright} 2014 by The American Society for Biochemistry and Molecular Biology, Inc.",
year = "2014",
month = sep,
day = "26",
doi = "10.1074/jbc.M114.587865",
language = "English",
volume = "289",
pages = "26752--26761",
journal = "J BIOL CHEM",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "39",

}

RIS

TY - JOUR

T1 - Nuclear factor of activated T cells regulates the expression of interleukin-4 in Th2 cells in an all-or-none fashion

AU - Köck, Juliana

AU - Kreher, Stephan

AU - Lehmann, Katrin

AU - Riedel, René

AU - Bardua, Markus

AU - Lischke, Timo

AU - Jargosch, Manja

AU - Haftmann, Claudia

AU - Bendfeldt, Hanna

AU - Hatam, Farahnaz

AU - Mashreghi, Mir-Farzin

AU - Baumgrass, Ria

AU - Radbruch, Andreas

AU - Chang, Hyun-Dong

N1 - © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

PY - 2014/9/26

Y1 - 2014/9/26

N2 - Th2 memory lymphocytes have imprinted their Il4 genes epigenetically for expression in dependence of T cell receptor restimulation. However, in a given restimulation, not all Th cells with a memory for IL-4 expression express IL-4. Here, we show that in reactivated Th2 cells, the transcription factors NFATc2, NF-kB p65, c-Maf, p300, Brg1, STAT6, and GATA-3 assemble at the Il4 promoter in Th2 cells expressing IL-4 but not in Th2 cells not expressing it. NFATc2 is critical for assembly of this transcription factor complex. Because NFATc2 translocation into the nucleus occurs in an all-or-none fashion, dependent on complete dephosphorylation by calcineurin, NFATc2 controls the frequencies of cells reexpressing Il4, translates analog differences in T cell receptor stimulation into a digital decision for Il4 reexpression, and instructs all reexpressing cells to express the same amount of IL-4. This analog-to-digital conversion may be critical for the immune system to respond to low concentrations of antigens.

AB - Th2 memory lymphocytes have imprinted their Il4 genes epigenetically for expression in dependence of T cell receptor restimulation. However, in a given restimulation, not all Th cells with a memory for IL-4 expression express IL-4. Here, we show that in reactivated Th2 cells, the transcription factors NFATc2, NF-kB p65, c-Maf, p300, Brg1, STAT6, and GATA-3 assemble at the Il4 promoter in Th2 cells expressing IL-4 but not in Th2 cells not expressing it. NFATc2 is critical for assembly of this transcription factor complex. Because NFATc2 translocation into the nucleus occurs in an all-or-none fashion, dependent on complete dephosphorylation by calcineurin, NFATc2 controls the frequencies of cells reexpressing Il4, translates analog differences in T cell receptor stimulation into a digital decision for Il4 reexpression, and instructs all reexpressing cells to express the same amount of IL-4. This analog-to-digital conversion may be critical for the immune system to respond to low concentrations of antigens.

KW - Active Transport, Cell Nucleus/physiology

KW - Adaptor Proteins, Signal Transducing/genetics

KW - Animals

KW - Cell Nucleus/genetics

KW - DNA Helicases/genetics

KW - E1A-Associated p300 Protein/genetics

KW - GATA3 Transcription Factor/genetics

KW - Gene Expression Regulation/physiology

KW - Interleukin-4/biosynthesis

KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, Transgenic

KW - NFATC Transcription Factors/genetics

KW - Nuclear Proteins/genetics

KW - Phosphorylation/physiology

KW - Receptors, Antigen, T-Cell/genetics

KW - Response Elements/physiology

KW - STAT6 Transcription Factor/genetics

KW - Th2 Cells/cytology

KW - Transcription Factor RelA/genetics

KW - Transcription Factors/genetics

U2 - 10.1074/jbc.M114.587865

DO - 10.1074/jbc.M114.587865

M3 - SCORING: Journal article

C2 - 25037220

VL - 289

SP - 26752

EP - 26761

JO - J BIOL CHEM

JF - J BIOL CHEM

SN - 0021-9258

IS - 39

ER -