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Nodular inflammatory foci are sites of T cell priming and control of murine cytomegalovirus infection in the neonatal lung

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Nodular inflammatory foci are sites of T cell priming and control of murine cytomegalovirus infection in the neonatal lung. / Stahl, Felix R; Heller, Katrin; Halle, Stephan; Keyser, Kirsten A; Busche, Andreas; Marquardt, Anja; Wagner, Karen; Boelter, Jasmin; Bischoff, Yvonne; Kremmer, Elisabeth; Arens, Ramon; Messerle, Martin; Förster, Reinhold.

in: PLOS PATHOG, Jahrgang 9, Nr. 12, 2013, S. e1003828.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Stahl, FR, Heller, K, Halle, S, Keyser, KA, Busche, A, Marquardt, A, Wagner, K, Boelter, J, Bischoff, Y, Kremmer, E, Arens, R, Messerle, M & Förster, R 2013, 'Nodular inflammatory foci are sites of T cell priming and control of murine cytomegalovirus infection in the neonatal lung', PLOS PATHOG, Jg. 9, Nr. 12, S. e1003828. https://doi.org/10.1371/journal.ppat.1003828

APA

Stahl, F. R., Heller, K., Halle, S., Keyser, K. A., Busche, A., Marquardt, A., Wagner, K., Boelter, J., Bischoff, Y., Kremmer, E., Arens, R., Messerle, M., & Förster, R. (2013). Nodular inflammatory foci are sites of T cell priming and control of murine cytomegalovirus infection in the neonatal lung. PLOS PATHOG, 9(12), e1003828. https://doi.org/10.1371/journal.ppat.1003828

Vancouver

Stahl FR, Heller K, Halle S, Keyser KA, Busche A, Marquardt A et al. Nodular inflammatory foci are sites of T cell priming and control of murine cytomegalovirus infection in the neonatal lung. PLOS PATHOG. 2013;9(12):e1003828. https://doi.org/10.1371/journal.ppat.1003828

Bibtex

@article{e7c08d51ae4d422b9ff408e406c670d4,
title = "Nodular inflammatory foci are sites of T cell priming and control of murine cytomegalovirus infection in the neonatal lung",
abstract = "Neonates, including mice and humans, are highly susceptible to cytomegalovirus (CMV) infection. However, many aspects of neonatal CMV infections such as viral cell tropism, spatio-temporal distribution of the pathogen as well as genesis of antiviral immunity are unknown. With the use of reporter mutants of the murine cytomegalovirus (MCMV) we identified the lung as a primary target of mucosal infection in neonatal mice. Comparative analysis of neonatal and adult mice revealed a delayed control of virus replication in the neonatal lung mucosa explaining the pronounced systemic infection and disease in neonates. This phenomenon was supplemented by a delayed expansion of CD8(+) T cell clones recognizing the viral protein M45 in neonates. We detected viral infection at the single-cell level and observed myeloid cells forming {"}nodular inflammatory foci{"} (NIF) in the neonatal lung. Co-localization of infected cells within NIFs was associated with their disruption and clearance of the infection. By 2-photon microscopy, we characterized how neonatal antigen-presenting cells (APC) interacted with T cells and induced mature adaptive immune responses within such NIFs. We thus define NIFs of the neonatal lung as niches for prolonged MCMV replication and T cell priming but also as sites of infection control.",
keywords = "Animals, Animals, Newborn, Antigen Presentation, Cells, Cultured, Cytomegalovirus Infections, Intestines, Lung, Lymphocyte Activation, Mice, Mice, Inbred C57BL, Mice, Transgenic, Muromegalovirus, Pneumonia, T-Lymphocytes",
author = "Stahl, {Felix R} and Katrin Heller and Stephan Halle and Keyser, {Kirsten A} and Andreas Busche and Anja Marquardt and Karen Wagner and Jasmin Boelter and Yvonne Bischoff and Elisabeth Kremmer and Ramon Arens and Martin Messerle and Reinhold F{\"o}rster",
year = "2013",
doi = "10.1371/journal.ppat.1003828",
language = "English",
volume = "9",
pages = "e1003828",
journal = "PLOS PATHOG",
issn = "1553-7366",
publisher = "Public Library of Science",
number = "12",

}

RIS

TY - JOUR

T1 - Nodular inflammatory foci are sites of T cell priming and control of murine cytomegalovirus infection in the neonatal lung

AU - Stahl, Felix R

AU - Heller, Katrin

AU - Halle, Stephan

AU - Keyser, Kirsten A

AU - Busche, Andreas

AU - Marquardt, Anja

AU - Wagner, Karen

AU - Boelter, Jasmin

AU - Bischoff, Yvonne

AU - Kremmer, Elisabeth

AU - Arens, Ramon

AU - Messerle, Martin

AU - Förster, Reinhold

PY - 2013

Y1 - 2013

N2 - Neonates, including mice and humans, are highly susceptible to cytomegalovirus (CMV) infection. However, many aspects of neonatal CMV infections such as viral cell tropism, spatio-temporal distribution of the pathogen as well as genesis of antiviral immunity are unknown. With the use of reporter mutants of the murine cytomegalovirus (MCMV) we identified the lung as a primary target of mucosal infection in neonatal mice. Comparative analysis of neonatal and adult mice revealed a delayed control of virus replication in the neonatal lung mucosa explaining the pronounced systemic infection and disease in neonates. This phenomenon was supplemented by a delayed expansion of CD8(+) T cell clones recognizing the viral protein M45 in neonates. We detected viral infection at the single-cell level and observed myeloid cells forming "nodular inflammatory foci" (NIF) in the neonatal lung. Co-localization of infected cells within NIFs was associated with their disruption and clearance of the infection. By 2-photon microscopy, we characterized how neonatal antigen-presenting cells (APC) interacted with T cells and induced mature adaptive immune responses within such NIFs. We thus define NIFs of the neonatal lung as niches for prolonged MCMV replication and T cell priming but also as sites of infection control.

AB - Neonates, including mice and humans, are highly susceptible to cytomegalovirus (CMV) infection. However, many aspects of neonatal CMV infections such as viral cell tropism, spatio-temporal distribution of the pathogen as well as genesis of antiviral immunity are unknown. With the use of reporter mutants of the murine cytomegalovirus (MCMV) we identified the lung as a primary target of mucosal infection in neonatal mice. Comparative analysis of neonatal and adult mice revealed a delayed control of virus replication in the neonatal lung mucosa explaining the pronounced systemic infection and disease in neonates. This phenomenon was supplemented by a delayed expansion of CD8(+) T cell clones recognizing the viral protein M45 in neonates. We detected viral infection at the single-cell level and observed myeloid cells forming "nodular inflammatory foci" (NIF) in the neonatal lung. Co-localization of infected cells within NIFs was associated with their disruption and clearance of the infection. By 2-photon microscopy, we characterized how neonatal antigen-presenting cells (APC) interacted with T cells and induced mature adaptive immune responses within such NIFs. We thus define NIFs of the neonatal lung as niches for prolonged MCMV replication and T cell priming but also as sites of infection control.

KW - Animals

KW - Animals, Newborn

KW - Antigen Presentation

KW - Cells, Cultured

KW - Cytomegalovirus Infections

KW - Intestines

KW - Lung

KW - Lymphocyte Activation

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Transgenic

KW - Muromegalovirus

KW - Pneumonia

KW - T-Lymphocytes

U2 - 10.1371/journal.ppat.1003828

DO - 10.1371/journal.ppat.1003828

M3 - SCORING: Journal article

C2 - 24348257

VL - 9

SP - e1003828

JO - PLOS PATHOG

JF - PLOS PATHOG

SN - 1553-7366

IS - 12

ER -