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New Safety Aspects in Corneal Donation-Studies on SARS-CoV-2-Positive Corneal Donors

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New Safety Aspects in Corneal Donation-Studies on SARS-CoV-2-Positive Corneal Donors. / Wille, Diana; Heinzelmann, Joana; Kehlen, Astrid; Lütgehetmann, Marc; Nörz, Dominik S; Siebolts, Udo; Mueller, Anke; Karrasch, Matthias; Hofmann, Nicola; Viestenz, Anja; Börgel, Martin; Kuhn, Ferenc; Viestenz, Arne.

in: J CLIN MED, Jahrgang 11, Nr. 12, 3312, 09.06.2022.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Wille, D, Heinzelmann, J, Kehlen, A, Lütgehetmann, M, Nörz, DS, Siebolts, U, Mueller, A, Karrasch, M, Hofmann, N, Viestenz, A, Börgel, M, Kuhn, F & Viestenz, A 2022, 'New Safety Aspects in Corneal Donation-Studies on SARS-CoV-2-Positive Corneal Donors', J CLIN MED, Jg. 11, Nr. 12, 3312. https://doi.org/10.3390/jcm11123312

APA

Wille, D., Heinzelmann, J., Kehlen, A., Lütgehetmann, M., Nörz, D. S., Siebolts, U., Mueller, A., Karrasch, M., Hofmann, N., Viestenz, A., Börgel, M., Kuhn, F., & Viestenz, A. (2022). New Safety Aspects in Corneal Donation-Studies on SARS-CoV-2-Positive Corneal Donors. J CLIN MED, 11(12), [3312]. https://doi.org/10.3390/jcm11123312

Vancouver

Wille D, Heinzelmann J, Kehlen A, Lütgehetmann M, Nörz DS, Siebolts U et al. New Safety Aspects in Corneal Donation-Studies on SARS-CoV-2-Positive Corneal Donors. J CLIN MED. 2022 Jun 9;11(12). 3312. https://doi.org/10.3390/jcm11123312

Bibtex

@article{e3f74643c6704aacb4367f98355041e6,
title = "New Safety Aspects in Corneal Donation-Studies on SARS-CoV-2-Positive Corneal Donors",
abstract = "In the tissue donation field, to prevent pathogen transmission, all donors are screened by postmortem swabs for SARS-CoV-2 using qRT-PCR. Corneas from donors who tested positive for SARS-CoV-2 were subjected to further investigations. Corneal transplants and culture medium from positive donors were cultured under appropriate safety conditions for further analyses. Cornea tissue samples, including sclera/limbus/cornea, and culture media were taken at different time points for testing for SARS-CoV-2 using qRT-PCR, immunohistochemistry (IHC) and subgenomic RNA (sgRNA) analysis. Between January and May 2021, in four donors with initial negative premortem rapid tests, SARS-CoV-2 was detected post-mortem using qRT-PCR. In these cases, SARS-CoV-2 was observed at the beginning of cultivation in both tissue and culture medium using qRT-PCR and IHC. The virus was mainly localized in the limbus epithelial cells, with a stable detection level. Premortem rapid tests are potentially insufficient to exclude SARS-CoV-2 infection in corneal donors. While, for SARS-CoV-2, the risk of infection via transplants is considered low, a residual risk remains for presymptomatic new infections. However, our investigations provide the first indications that, with organ cultures, the risk of virus transmission is minimized due to the longer minimum culture period.",
author = "Diana Wille and Joana Heinzelmann and Astrid Kehlen and Marc L{\"u}tgehetmann and N{\"o}rz, {Dominik S} and Udo Siebolts and Anke Mueller and Matthias Karrasch and Nicola Hofmann and Anja Viestenz and Martin B{\"o}rgel and Ferenc Kuhn and Arne Viestenz",
year = "2022",
month = jun,
day = "9",
doi = "10.3390/jcm11123312",
language = "English",
volume = "11",
journal = "J CLIN MED",
issn = "2077-0383",
publisher = "MDPI AG",
number = "12",

}

RIS

TY - JOUR

T1 - New Safety Aspects in Corneal Donation-Studies on SARS-CoV-2-Positive Corneal Donors

AU - Wille, Diana

AU - Heinzelmann, Joana

AU - Kehlen, Astrid

AU - Lütgehetmann, Marc

AU - Nörz, Dominik S

AU - Siebolts, Udo

AU - Mueller, Anke

AU - Karrasch, Matthias

AU - Hofmann, Nicola

AU - Viestenz, Anja

AU - Börgel, Martin

AU - Kuhn, Ferenc

AU - Viestenz, Arne

PY - 2022/6/9

Y1 - 2022/6/9

N2 - In the tissue donation field, to prevent pathogen transmission, all donors are screened by postmortem swabs for SARS-CoV-2 using qRT-PCR. Corneas from donors who tested positive for SARS-CoV-2 were subjected to further investigations. Corneal transplants and culture medium from positive donors were cultured under appropriate safety conditions for further analyses. Cornea tissue samples, including sclera/limbus/cornea, and culture media were taken at different time points for testing for SARS-CoV-2 using qRT-PCR, immunohistochemistry (IHC) and subgenomic RNA (sgRNA) analysis. Between January and May 2021, in four donors with initial negative premortem rapid tests, SARS-CoV-2 was detected post-mortem using qRT-PCR. In these cases, SARS-CoV-2 was observed at the beginning of cultivation in both tissue and culture medium using qRT-PCR and IHC. The virus was mainly localized in the limbus epithelial cells, with a stable detection level. Premortem rapid tests are potentially insufficient to exclude SARS-CoV-2 infection in corneal donors. While, for SARS-CoV-2, the risk of infection via transplants is considered low, a residual risk remains for presymptomatic new infections. However, our investigations provide the first indications that, with organ cultures, the risk of virus transmission is minimized due to the longer minimum culture period.

AB - In the tissue donation field, to prevent pathogen transmission, all donors are screened by postmortem swabs for SARS-CoV-2 using qRT-PCR. Corneas from donors who tested positive for SARS-CoV-2 were subjected to further investigations. Corneal transplants and culture medium from positive donors were cultured under appropriate safety conditions for further analyses. Cornea tissue samples, including sclera/limbus/cornea, and culture media were taken at different time points for testing for SARS-CoV-2 using qRT-PCR, immunohistochemistry (IHC) and subgenomic RNA (sgRNA) analysis. Between January and May 2021, in four donors with initial negative premortem rapid tests, SARS-CoV-2 was detected post-mortem using qRT-PCR. In these cases, SARS-CoV-2 was observed at the beginning of cultivation in both tissue and culture medium using qRT-PCR and IHC. The virus was mainly localized in the limbus epithelial cells, with a stable detection level. Premortem rapid tests are potentially insufficient to exclude SARS-CoV-2 infection in corneal donors. While, for SARS-CoV-2, the risk of infection via transplants is considered low, a residual risk remains for presymptomatic new infections. However, our investigations provide the first indications that, with organ cultures, the risk of virus transmission is minimized due to the longer minimum culture period.

U2 - 10.3390/jcm11123312

DO - 10.3390/jcm11123312

M3 - SCORING: Journal article

C2 - 35743381

VL - 11

JO - J CLIN MED

JF - J CLIN MED

SN - 2077-0383

IS - 12

M1 - 3312

ER -