Neural cell type-specific responses to glycomimetic functionalized collagen.
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Neural cell type-specific responses to glycomimetic functionalized collagen. / Masand, Shirley N; Perron, Isaac J; Schachner, Melitta; Shreiber, David I.
in: BIOMATERIALS, Jahrgang 33, Nr. 3, 3, 2012, S. 790-797.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Neural cell type-specific responses to glycomimetic functionalized collagen.
AU - Masand, Shirley N
AU - Perron, Isaac J
AU - Schachner, Melitta
AU - Shreiber, David I
PY - 2012
Y1 - 2012
N2 - Despite their noted functional role, glycans have had limited therapeutic use due to difficulties in synthesis and quick degradation in vivo. The recent discovery of glycomimetics has provided new opportunities for their application. In this study, we have functionalized type I collagen with peptide mimics of two glycans: (1) polysialic acid (PSA) and (2) an epitope first discovered on human natural killer cells (HNK-1). These glycans and their glycomimetic counterparts have been shown to be important regulators of repair following injury through their unique and phenotypically specific effects on neural behavior. We show that these molecules retain their bioactivity following functionalization to the collagen backbone. Grafted HNK-1 encouraged motor neuron outgrowth, while grafted PSA encouraged sensory and motor neuron outgrowth and enhanced Schwann cell proliferation and process extension. These data support the potential of glycomimetic-functionalized collagen as a biomaterial strategy to increase the efficiency of synaptic reconnection following nervous system injury.
AB - Despite their noted functional role, glycans have had limited therapeutic use due to difficulties in synthesis and quick degradation in vivo. The recent discovery of glycomimetics has provided new opportunities for their application. In this study, we have functionalized type I collagen with peptide mimics of two glycans: (1) polysialic acid (PSA) and (2) an epitope first discovered on human natural killer cells (HNK-1). These glycans and their glycomimetic counterparts have been shown to be important regulators of repair following injury through their unique and phenotypically specific effects on neural behavior. We show that these molecules retain their bioactivity following functionalization to the collagen backbone. Grafted HNK-1 encouraged motor neuron outgrowth, while grafted PSA encouraged sensory and motor neuron outgrowth and enhanced Schwann cell proliferation and process extension. These data support the potential of glycomimetic-functionalized collagen as a biomaterial strategy to increase the efficiency of synaptic reconnection following nervous system injury.
M3 - SCORING: Journal article
VL - 33
SP - 790
EP - 797
JO - BIOMATERIALS
JF - BIOMATERIALS
SN - 0142-9612
IS - 3
M1 - 3
ER -
