Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging
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Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging. / Uetrecht, Charlotte; Lorenzen, Kristina; Kitel, Matthäus; Heidemann, Johannes; Robinson Spencer, Jesse Huron; Schlüter, Hartmut; Schulz, Joachim.
in: J SYNCHROTRON RADIAT, Jahrgang 26, Nr. Pt 3, 01.05.2019, S. 653-659.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging
AU - Uetrecht, Charlotte
AU - Lorenzen, Kristina
AU - Kitel, Matthäus
AU - Heidemann, Johannes
AU - Robinson Spencer, Jesse Huron
AU - Schlüter, Hartmut
AU - Schulz, Joachim
N1 - open access.
PY - 2019/5/1
Y1 - 2019/5/1
N2 - The SPB/SFX instrument at the European XFEL provides unique conditions for single-particle imaging (SPI) experiments due to its high brilliance, nano-focus and unique pulse structure. Promising initial results provided by the international LCLS (Linac Coherent Light Source) SPI initiative highlight the potential of SPI. Current available injection methods generally have high sample consumption and do not provide any options for pulsing, selection or orientation of particles, which poses a problem for data evaluation. Aerosol-injector-based sample delivery is the current method of choice for SPI experiments, although, to a lesser extent, electrospray and electrospinning are used. Single particles scatter only a limited number of photons providing a single orientation for data evaluation, hence large datasets are required from particles in multiple orientations in order to reconstruct a structure. Here, a feasibility study demonstrates that nano-electrospray ionization, usually employed in biomolecular mass spectrometry, provides enough ion flux for SPI experiments. A novel instrument setup at the SPB/SFX instrument is proposed, which has the benefit of extremely low background while delivering mass over charge and conformation-selected ions for SPI.
AB - The SPB/SFX instrument at the European XFEL provides unique conditions for single-particle imaging (SPI) experiments due to its high brilliance, nano-focus and unique pulse structure. Promising initial results provided by the international LCLS (Linac Coherent Light Source) SPI initiative highlight the potential of SPI. Current available injection methods generally have high sample consumption and do not provide any options for pulsing, selection or orientation of particles, which poses a problem for data evaluation. Aerosol-injector-based sample delivery is the current method of choice for SPI experiments, although, to a lesser extent, electrospray and electrospinning are used. Single particles scatter only a limited number of photons providing a single orientation for data evaluation, hence large datasets are required from particles in multiple orientations in order to reconstruct a structure. Here, a feasibility study demonstrates that nano-electrospray ionization, usually employed in biomolecular mass spectrometry, provides enough ion flux for SPI experiments. A novel instrument setup at the SPB/SFX instrument is proposed, which has the benefit of extremely low background while delivering mass over charge and conformation-selected ions for SPI.
KW - Journal Article
U2 - 10.1107/S1600577519002686
DO - 10.1107/S1600577519002686
M3 - SCORING: Journal article
C2 - 31074428
VL - 26
SP - 653
EP - 659
JO - J SYNCHROTRON RADIAT
JF - J SYNCHROTRON RADIAT
SN - 0909-0495
IS - Pt 3
ER -
