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Nanoencapsulated capsaicin changes migration behavior and morphology of madin darby canine kidney cell monolayers

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Nanoencapsulated capsaicin changes migration behavior and morphology of madin darby canine kidney cell monolayers. / Kaiser, Mathias; Pohl, Luisa; Ketelhut, Steffi; Kastl, Lena; Gorzelanny, Christian; Götte, Martin; Schnekenburger, Jürgen; Goycoolea, Francisco M; Kemper, Björn.

in: PLOS ONE, Jahrgang 12, Nr. 11, 2017, S. e0187497.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Kaiser, M, Pohl, L, Ketelhut, S, Kastl, L, Gorzelanny, C, Götte, M, Schnekenburger, J, Goycoolea, FM & Kemper, B 2017, 'Nanoencapsulated capsaicin changes migration behavior and morphology of madin darby canine kidney cell monolayers', PLOS ONE, Jg. 12, Nr. 11, S. e0187497. https://doi.org/10.1371/journal.pone.0187497

APA

Kaiser, M., Pohl, L., Ketelhut, S., Kastl, L., Gorzelanny, C., Götte, M., Schnekenburger, J., Goycoolea, F. M., & Kemper, B. (2017). Nanoencapsulated capsaicin changes migration behavior and morphology of madin darby canine kidney cell monolayers. PLOS ONE, 12(11), e0187497. https://doi.org/10.1371/journal.pone.0187497

Vancouver

Kaiser M, Pohl L, Ketelhut S, Kastl L, Gorzelanny C, Götte M et al. Nanoencapsulated capsaicin changes migration behavior and morphology of madin darby canine kidney cell monolayers. PLOS ONE. 2017;12(11):e0187497. https://doi.org/10.1371/journal.pone.0187497

Bibtex

@article{753ddf19ba904a8da5d22c0ee9b3af8b,
title = "Nanoencapsulated capsaicin changes migration behavior and morphology of madin darby canine kidney cell monolayers",
abstract = "We have developed a drug delivery nanosystem based on chitosan and capsaicin. Both substances have a wide range of biological activities. We investigated the nanosystem's influence on migration and morphology of Madin Darby canine kidney (MDCK-C7) epithelial cells in comparison to the capsaicin-free nanoformulation, free capsaicin, and control cells. For minimally-invasive quantification of cell migration, we applied label-free digital holographic microscopy (DHM) and single-cell tracking. Moreover, quantitative DHM phase images were used as novel stain-free assay to quantify the temporal course of global cellular morphology changes in confluent cell layers. Cytoskeleton alterations and tight junction protein redistributions were complementary analyzed by fluorescence microscopy. Calcium influx measurements were conducted to characterize the influence of the nanoformulations and capsaicin on ion channel activities. We found that both, capsaicin-loaded and unloaded chitosan nanocapsules, and also free capsaicin, have a significant impact on directed cell migration and cellular motility. Increase of velocity and directionality of cell migration correlates with changes in the cell layer surface roughness, tight junction integrity and cytoskeleton alterations. Calcium influx into cells occurred only after nanoformulation treatment but not upon addition of free capsaicin. Our results pave the way for further studies on the biological significance of these findings and potential biomedical applications, e.g. as drug and gene carriers.",
keywords = "Animals, Calcium, Capsaicin, Cell Movement, Dogs, Madin Darby Canine Kidney Cells, Microscopy, Fluorescence, Nanocapsules, Journal Article",
author = "Mathias Kaiser and Luisa Pohl and Steffi Ketelhut and Lena Kastl and Christian Gorzelanny and Martin G{\"o}tte and J{\"u}rgen Schnekenburger and Goycoolea, {Francisco M} and Bj{\"o}rn Kemper",
year = "2017",
doi = "10.1371/journal.pone.0187497",
language = "English",
volume = "12",
pages = "e0187497",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",

}

RIS

TY - JOUR

T1 - Nanoencapsulated capsaicin changes migration behavior and morphology of madin darby canine kidney cell monolayers

AU - Kaiser, Mathias

AU - Pohl, Luisa

AU - Ketelhut, Steffi

AU - Kastl, Lena

AU - Gorzelanny, Christian

AU - Götte, Martin

AU - Schnekenburger, Jürgen

AU - Goycoolea, Francisco M

AU - Kemper, Björn

PY - 2017

Y1 - 2017

N2 - We have developed a drug delivery nanosystem based on chitosan and capsaicin. Both substances have a wide range of biological activities. We investigated the nanosystem's influence on migration and morphology of Madin Darby canine kidney (MDCK-C7) epithelial cells in comparison to the capsaicin-free nanoformulation, free capsaicin, and control cells. For minimally-invasive quantification of cell migration, we applied label-free digital holographic microscopy (DHM) and single-cell tracking. Moreover, quantitative DHM phase images were used as novel stain-free assay to quantify the temporal course of global cellular morphology changes in confluent cell layers. Cytoskeleton alterations and tight junction protein redistributions were complementary analyzed by fluorescence microscopy. Calcium influx measurements were conducted to characterize the influence of the nanoformulations and capsaicin on ion channel activities. We found that both, capsaicin-loaded and unloaded chitosan nanocapsules, and also free capsaicin, have a significant impact on directed cell migration and cellular motility. Increase of velocity and directionality of cell migration correlates with changes in the cell layer surface roughness, tight junction integrity and cytoskeleton alterations. Calcium influx into cells occurred only after nanoformulation treatment but not upon addition of free capsaicin. Our results pave the way for further studies on the biological significance of these findings and potential biomedical applications, e.g. as drug and gene carriers.

AB - We have developed a drug delivery nanosystem based on chitosan and capsaicin. Both substances have a wide range of biological activities. We investigated the nanosystem's influence on migration and morphology of Madin Darby canine kidney (MDCK-C7) epithelial cells in comparison to the capsaicin-free nanoformulation, free capsaicin, and control cells. For minimally-invasive quantification of cell migration, we applied label-free digital holographic microscopy (DHM) and single-cell tracking. Moreover, quantitative DHM phase images were used as novel stain-free assay to quantify the temporal course of global cellular morphology changes in confluent cell layers. Cytoskeleton alterations and tight junction protein redistributions were complementary analyzed by fluorescence microscopy. Calcium influx measurements were conducted to characterize the influence of the nanoformulations and capsaicin on ion channel activities. We found that both, capsaicin-loaded and unloaded chitosan nanocapsules, and also free capsaicin, have a significant impact on directed cell migration and cellular motility. Increase of velocity and directionality of cell migration correlates with changes in the cell layer surface roughness, tight junction integrity and cytoskeleton alterations. Calcium influx into cells occurred only after nanoformulation treatment but not upon addition of free capsaicin. Our results pave the way for further studies on the biological significance of these findings and potential biomedical applications, e.g. as drug and gene carriers.

KW - Animals

KW - Calcium

KW - Capsaicin

KW - Cell Movement

KW - Dogs

KW - Madin Darby Canine Kidney Cells

KW - Microscopy, Fluorescence

KW - Nanocapsules

KW - Journal Article

U2 - 10.1371/journal.pone.0187497

DO - 10.1371/journal.pone.0187497

M3 - SCORING: Journal article

C2 - 29107993

VL - 12

SP - e0187497

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 11

ER -